iQ™ SYBR® Green Supermix, 1,000 x 50 µl rxns, 25 ml (5 x 5 ml) from Bio-Rad

Product Specs
ItemiQ™ SYBR^® Green Supermix, 1,000 x 50 µl rxns, 25 ml (5 x 5 ml)
CompanyBio-Rad
Price Pricing Info Supplier Page View Company Product Page
Catalog Number1708886
TypeSupermix
Brand/TrademarkLSG GXD

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) Antiviral functionalization of cellulose using tannic acid and tannin-rich extractsFront MicrobiolDecember 6, 2023Marjo Haapakoski, Aleksei Emelianov, Dhanik Reshamwala, Mira Laajala, Jenni Tienaho, Petri Kilpeläinen, Jaana Liimatainen, Tuula Jyske, Mika Pettersson, Varpu Marjomäkiexecuted at 42°C for 60 min with final 10 min at 70°C. A master mix containing SYBR Green Supermix (#1708886, BioRad), 600 nM HCoV-OC43 forward (5′-TGTTAGGCCRATAATTGAGGAC), and reverse primer (5′-AATGTAAAGATGRCCGCGTATT)

(2) Absence of gut microbiota reduces neonatal survival and exacerbates liver disease in Cyp2c70-deficient mice with a human-like bile acid compositionClinical ScienceJuly 14, 2023Wilhelm Sjöland, Annika Wahlström, Kassem Makki, Marc Schöler, Antonio Molinaro, Lisa Olsson, Thomas Uwe Greiner, Robert Caesar, Jan Freark de Boer, Folkert Kuipers, et al.products were diluted 7x before use in subsequent reactions. 1× iQ™ SYBR® Green Supermix (Bio-Rad, 1708886) was used for qRT-PCR at final reaction volumes of 10 µl. 900 nM gene-specific primers were used in

(3) Therapeutic potential of a novel peripherally restricted CB1R inverse agonist on the progression of diabetic nephropathyFrontiers in NephrologyMarch 28, 2023Laetitia Jacquot, Océane Pointeau, Célia Roger-Villeboeuf, Patricia Passilly-Degrace, Rim Belkaid, Isaline Regazzoni, Julia Leemput, Chloé Buch, Laurent Demizieux, Bruno Vergès, et al.polymerase chain reaction (RT-qPCR; Bio-Rad Laboratories, #1708841). A Sybr Green Supermix kit (Bio-Rad Laboratories, #1708886) with QuantStudio 3 Real Time PCR System (ThermoFisher, Illkirch-Graffenstaden, France) were used for

(4) Multiphase coalescence mediates Hippo pathway activationCellNovember 1, 2022Li Wang, Kyungsuk Choi, Ting Su, Bing Li, Xiaofeng Wu, Ruihui Zhang, Jordan H. Driskill, Hongde Li, Huiyan Lei, Pengfei Guo, et al.Reverse Transcription Kit,Thermo Fisher Scientific,Cat# 14368814 iQ SYBR Green Supermix,Bio-Rad,Cat# 1708886 ER-Tracker Red,Thermo Fisher Scientific,Cat# E34250 ER-Tracker Green,Thermo Fisher Scientific,Cat#

(5) Identification of stable housekeeping genes for induced pluripotent stem cells and -derived endothelial cells for drug testingScientific ReportsSeptember 28, 2022Sheena L. M. Ong, Hans J. Baelde, David G. P. van IJzendoorn, Judith V. M. G. Bovée, Karoly SzuhaiiScript™ cDNA Synthesis Kit (Bio-rad, 1708891). qRT-PCR reactions were carried out with iQ™ SYBR® Green Supermix (Bio-rad, 1708886). The samples that were used to determine the stability of reference genes are iPSCWT, iPSCSERPINE1-FOSB,

(6) CRISPR-mediated activation of autism gene Itgb3 restores cortical network excitability via mGluR5 signalingMolecular Therapy - Nucleic AcidsSeptember 13, 2022Fanny Jaudon, Agnes Thalhammer, Lorena Zentilin, Lorenzo A. CingolaniqRT-PCR was performed in triplicate with 10 ng of template cDNA using iQ SYBR Green Supermix (cat. no. 1708886, Biorad) on a CFX96 Real-Time PCR Detection System (Biorad) with the following universal conditions:

(7) Myeloid cell-specific topoisomerase 1 inhibition using DNA origami mitigates neuroinflammationEMBO reportsJuly 5, 2022Zhu K, Wang Y, Sarlus H, Geng K, Nutma E, Sun J, Kung SY, Bay C, Han J, Min JH, et al.the standard protocol provided. Amplifications were conducted using SYBR green (BioRad Laboratories, 1708886) according to the manufacturer’s instructions and the 384‐well plates were run in a BioRad CFX384 Touch Real‐Time PCR Detection System

(8) STING is an intrinsic checkpoint inhibitor that restrains the T(H)17 cell pathogenic programCell reportsMay 24, 2022Damasceno LEA, Cebinelli GCM, Fernandes MF, Nascimento DC, Públio GA, Vinolo MAR, Oliveira SC, Sparwasser T, Cunha TM, Cunha FQ, et al.65-0865-14 Power SYBR Green Master Mix,Applied Biosystems,Cat# 4368708 iQ™ SYBR® Green Supermix,Bio-Rad,Cat# 1708886 PMA,Sigma-Aldrich,Cat# P1585 Ionomycin,Sigma-Aldrich,Cat# I0634 BD GolgiStop™ (containing Monensin),BD Biosciences

(9) CRISPR-Mediated Activation of αV Integrin Subtypes Promotes Neuronal Differentiation of Neuroblastoma Neuro2a CellsFrontiers in Genome EditingApril 12, 2022Sara Riccardi, Lorenzo A. Cingolani, Fanny JaudonRT-qPCR was performed in triplicate with 10 ng of template cDNA using iQTM SYBR® Green Supermix (Cat. No. 1708886, Biorad) on a CFX96 Real-Time PCR Detection System (Biorad) with the following universal conditions:

(10) MiR-194-5p enhances the sensitivity of nonsmall-cell lung cancer to doxorubicin through targeted inhibition of hypoxia-inducible factor-1World Journal of Surgical OncologyDecember 1, 2021Mengning Xia, Lili Sheng, Wei Qu, Xue Xue, Hucheng Chen, Guoyan Zheng, Weigang Chenreverse transcription kit (Takara Bio, Korea). Real-time PCR was performed by IQ SYBR Green Supermix (1708886, Bio-Rad, USA) in line with the supplier’s guidance. The primers below were applied: miR-194-5p forward

(11) Expanding the Spectrum of EWSR1-NFATC2-rearranged Benign Tumors: A Common Genomic Abnormality in Vascular Malformation/Hemangioma and Simple Bone CystAmerican Journal of Surgical PathologyDecember 1, 2021Sheena L.M. Ong et al.(Roche, 109118) was used to synthesize 1 µg of total RNA into cDNA. Before real-time PCR (Bio-Rad, 1708886), cDNA samples were diluted 5× with milliQ water. Samples were run in technical duplicates. Primers

(12) Protein arginine methyltransferase 8 modulates mitochondrial bioenergetics and neuroinflammation after hypoxic stressJournal of neurochemistryNovember 1, 2021Couto E Silva A, Wu CY, Clemons GA, Acosta CH, Chen CT, Possoit HE, Citadin CT, Lee RH, Brown JI, Frankel A, et al.qPCR was performed in CFX96 Real-Time PCR Detection System using iQ™ SYBR® Green Supermix (Cat. No. 1708886, Bio-Rad, Hercules, CA). The PCR mix consisted in 0.4 μL of cDNA, 10 μL of iQ™ SYBR® Green Supermix

(13) Serum deprivation limits loss and promotes recovery of tenogenic phenotype in tendon cell culture systemsJournal of Orthopaedic ResearchJuly 1, 2021Marc van Vijven, Stefania L. Wunderli, Keita Ito, Jess G. Snedeker, Jasper Foolenthe CFX 384 Thermal Cycler (BioRad) for 40 cycles, using corresponding iQ SYBR Green Supermix (BioRad 1708886). C t‐values were normalized to the mean C t‐values of housekeeping genes Rpl4 and Rps29 and native

(14) The chaperone-binding activity of the mitochondrial surface receptor Tom70 protects the cytosol against mitoprotein-induced stressCell ReportsApril 6, 2021Sandra Backes, Yury S. Bykov, Tamara Flohr, Markus Räschle, Jialin Zhou, Svenja Lenhard, Lena Krämer, Timo Mühlhaus, Chen Bibi, Cosimo Jann, et al.kit,Ambion,AM1907 qScript cDNA Synthesis Kit,Quanta Biosciences,95047 iQ SYBR Green Supermix,BioRad,1708886 Pierce BCA Protein Assay Kit,Thermo Scientific,23225 TMT10plex isobaric label reagent set,Thermo Scientific,90111 Deposited

(15) A New Horizon of Liquid Biopsy in Thymic Epithelial Tumors: The Potential Utility of Circulating Cell-Free DNAFrontiers in OncologyFebruary 4, 2021Margaret Ottaviano et al.detail, for each sample, reactions were performed in duplicates using iQ SYBR Green Supermix (ref. 1708886 Bio-rad laboratories) on a QuantStudio 7 Flex Real-time PCR detection system (ref. 4485701 ThermoFischer)

(16) Low-Expressing Synucleinopathy Mouse Models Based on Oligomer-Forming Mutations and C-Terminal Truncation of α-SynucleinFrontiers in neuroscienceJanuary 1, 2021Martinez Hernandez A, Silbern I, Geffers I, Tatenhorst L, Becker S, Urlaub H, Zweckstetter M, Griesinger C, Eichele G.per mouse. qPCR was performed using iQTM SYBR Green Supermix (BioRad, Hercules, United States, Cat. # 1708886) on an iCycler CFX96 Real-Time PCR Detection System thermocycler (BioRad). Primers used were 50 – AAG

(17) TEX10 Promotes the Tumorigenesis and Radiotherapy Resistance of Urinary Bladder Carcinoma by Stabilizing XRCC6Journal of immunology researchJanuary 1, 2021Luo S, Wang W, Feng J, Li R.accordance with procedure's guideline. The researchers carried out qPCR under SYBR-Green (cat. no. 1708886; Bio-Rad Laboratories, Inc.) onto one iQ5 Multicolor Real-Time PCR Detection mechanism (Bio-Rad Laboratories,

(18) Endothelial specific YY1 deletion restricts tumor angiogenesis and tumor growthScientific reportsNovember 24, 2020Liu H, Qiu Y, Pei X, Chitteti R, Steiner R, Zhang S, Jin ZG.performed with a Bio-Rad iQ5 real-time PCR thermal cycler, using iQ SYBR Green Supermix (Bio-Rad, Cat No. 1708886) for relative mRNA quantification. All primer sequences were listed in Table S. The comparative cycle

(19) TENET 2.0: Identification of key transcriptional regulators and enhancers in lung adenocarcinomaPLOS GeneticsSeptember 14, 2020Daniel J. Mullen, Chunli Yan, Diane S. Kang, Beiyun Zhou, Zea Borok, Crystal N. Marconett, Peggy J. Farnham, Ite A. Offringa, Suhn Kyong Rhieand expression levels of FOXM1 and MYBL2 were checked with qRT-PCR using SYBR Green Supermix (Cat # 1708886, Bio-Rad, CA, USA) with the listed primers (S11 Table). RNA-seq was performed using 150 bp paired-end

(20) An Optimized Chromatin Immunoprecipitation Protocol for Quantification of Protein-DNA InteractionsSTAR protocolsJune 19, 2020de Jonge WJ, Brok M, Kemmeren P, Holstege FCP.,Perkin Elmer,Cat#NEL105001EA High Sensitivity DNA Kit,Agilent,Cat#5067-4626 IQ SYBR Green super mix,Bio-Rad,Cat#1708886 Precision Blue™ Real-Time PCR Dye,Bio-Rad,Cat#1725555 ,, Software and Algorithms,Software and Algorithms,Software

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Cellular and molecular gastroenterology and hepatology

iQ™ SYBR^® Green Supermix, 1,000 x 50 µl rxns, 25 ml (5 x 5 ml) from Bio-Rad

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iQ™ SYBR^® Green Supermix, 1,000 x 50 µl rxns, 25 ml (5 x 5 ml) from Bio-Rad

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