Nano-Glo Endurazine Live Cell Substrate from Promega

Product Specs
ItemNano-Glo Endurazine Live Cell Substrate
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberN2570
Quantity0.1ml

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Bioluminescence Assay of Lysine Deacylase Sirtuin ActivitybioRxivAugust 18, 2023Alexandria N. Van Scoyk, Orlando Antelope, Anca Franzini, Donald E. Ayer, Randall T. Peterson, Anthony D. Pomicter, Shawn C. Owen, Michael W. Deiningerzebrafish (Danio rerio) and injected at the 1-cell stage. For preliminary testing of endurazine (Promega, N2570) and furimazine (Promega, N1120), 5 μL reactions were made by adding 4 μL LgBiT (Promega, N1120), 0.5

(2) Probing expression of E-selectin using CRISPR-Cas9-mediated tagging with HiBiT in human endothelial cellsiScienceJuly 21, 2023Lydia Ogrodzinski, Simon Platt, Joelle Goulding, Cameron Alexander, Tracy D. Farr, Jeanette Woolard, Stephen J. Hill, Laura E. Kilpatrickhours, experiments were conducted in the presence of a caged nanoluciferase substrate (Endurazine™; N2570, Promega USA) in Opti-MEM medium. Clonal HiBiT-TERT2-HUVECs (clone C8) were seeded onto 0.1% gelatin

(3) A stem cell zoo uncovers intracellular scaling of developmental tempo across mammalsCell stem cellJuly 6, 2023Lázaro J, Costanzo M, Sanaki-Matsumiya M, Girardot C, Hayashi M, Hayashi K, Diecke S, Hildebrandt TB, Lazzari G, Wu J, et al.M6145 Insulin,Sigma-Aldrich,Cat# 91077C-1G CD concentrated lipid,Gibco,Cat# 11905-031 Furimazine,Promega,Cat# N2570 Sodium Azide,Sigma-Aldrich,Cat# 71290-10G Laminin,Amsbio,Cat# AMS.892 021 Knockout serum replacement,Gibco,Cat#

(4) STING signalling is terminated through ESCRT-dependent microautophagy of vesicles originating from recycling endosomesNature Cell BiologyMarch 13, 2023Yoshihiko Kuchitsu, Kojiro Mukai, Rei Uematsu, Yuki Takaada, Ayumi Shinojima, Ruri Shindo, Tsumugi Shoji, Shiori Hamano, Emari Ogawa, Ryota Sato, et al.expressing LgBiT-STING and SmBiT-Tsg101 (WT or ΔUEV) were treated with Nano-Glo Endurazine substrate (N2570, Promega) for 2 h at 37 °C. Cells were then stimulated with vehicle (DMSO) or DMXAA for 4 h. The luciferase

(5) Targeted Degradation of Androgen Receptor by VNPP433-3β in Castration-Resistant Prostate Cancer Cells Implicates Interaction with E3 Ligase MDM2 Resulting in Ubiquitin-Proteasomal DegradationCancers (Basel)February 14, 2023Elizabeth Thomas, Retheesh S. Thankan, Puranik Purushottamachar, David J. Weber, Vincent C. O. NjarThe second day, the media was replaced by 1× solution of Endurazine (Nano-Glo® Endurazine™ Promega # N2570) prepared in CO2-independent medium (Gibco # 18045088). The plates were incubated at 37 °C and 5% CO2

(6) Discovery of a potent BTK and IKZF1/3 triple degrader through reversible covalent BTK PROTAC developmentCurrent Research in Chemical BiologyJanuary 30, 2023Xin Yu, Wen-Hao Guo, Hanfeng Lin, Ran Cheng, Erika Y. Monroy, Feng Jin, Lang Ding, Dong Lu, Xiaoli Qi, Meng C. Wang, et al.and treated with 40 nM or 200 nM of indicated compounds plus endurazine (1:2000, Promega, Cat. No. N2570) and extracellular nanoluc inhibitor (1:2000, Promega, Cat. No. N2162), after which the real-time luminescence

(7) Multimodal surveillance of SARS-CoV-2 at a university enables development of a robust outbreak response frameworkMedDecember 9, 2022Petros BA, Paull JS, Tomkins-Tinch CH, Loftness BC, DeRuff KC, Nair P, Gionet GL, Benz A, Brock-Fisher T, Hughes M, et al."TrypLE Express Enzyme (1X), no phenol red",ThermoFisher Scientific,#12604013 Nano-Glo Endurazine Live Cell Substrates,ProMega,#N2570 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, MycoAlert Mycoplasma Detection kit

(8) Optogenetic induction of hibernation-like state with modified human Opsin4 in miceCell Reports MethodsNovember 21, 2022Tohru M. Takahashi, Arisa Hirano, Takeshi Kanda, Viviane M. Saito, Hiroto Ashitomi, Kazumasa Z. Tanaka, Yasufumi Yokoshiki, Kosaku Masuda, Masashi Yanagisawa, Kaspar E. Vogt, et al.recording medium. Twenty-four hours after the medium change, the substrate of luciferase (Endurazine, N2570, Promega) was added to the recording medium following the manufacturer’s protocol. Bioluminescence was

(9) A kinetic intra-cellular assay (KICA) to measure quantitative compound binding kinetics within living cellsSTAR ProtocolsMarch 18, 2022Charles S. Lay, Daniel A. Thomas, John P. Evans, Emma J. Jones, Kelly M. Gatfield, Peter D. Craggsavailable from Promega and can be used to extend luminescence signal stability (Cat. numbers N2580 and N2570 respectively). Problem 2 Regarding Quantification and statistical analysis: If the measurable affinity

(10) Establishment of a developmental toxicity assay based on human iPSC reporter to detect FGF signal disruptioniScienceFebruary 18, 2022Kanno S, Okubo Y, Kageyama T, Yan L, Kitajima S, Fukuda J.experiments were performed with the APEL2 medium supplemented with 1% NanoLuc substrate Nano-Glo® Endurazine (N2570, Promega). After the application of the substrate-containing medium, the cells were equilibrated for

(11) Targeted Protein Degradation Phenotypic Studies Using HaloTag CRISPR/Cas9 Endogenous Tagging Coupled with HaloPROTAC3Current Protocols in PharmacologyDecember 17, 2020Elizabeth A. Caine, Sarah D. Mahan, Rebecca L. Johnson, Amanda N. Nieman, Ngan Lam, Curtis R. Warren, Kristin M. Riching, Marjeta Urh, Danette L. DanielsLgBiT Expression Vector (Promega, cat. no. N2681)Nano‐Glo Endurazine Live Cell Substrate (Promega, cat. no. N2570/1/2)CO2‐independent medium (Gibco, cat. no. 18045‐088)FBS (VWR, cat. no. 89510‐194) Six‐well platesAppropriate

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