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Protein Biochemistry
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Incucyte® Caspase-3/7 Green Reagent
Incucyte® Caspase-3/7 Green Reagent from Sartorius
Be the first to write a review!
Citations:
(36)
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Supplier Page from
Sartorius for
Incucyte® Caspase-3/7 Green Reagent
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Company Info
Citations
Reviews
Product Specs
Item
Incucyte® Caspase-3/7 Green Reagent
Company
Sartorius
Price
Supplier Page
View Company Product Page
Catalog Number
4440
Quantity
1 vial
Applications
Apoptosis, Cell Imaging
Type
Reagent
Add to Compare List
Local Office
Sartorius
5 Orville Drive
Bohemia
,
NY
11716
United States
Company Profile
International Office
Sartorius
Otto-Brenner-Straße 20
37079 Göttingen
Germany
Phone
: +49 551 308 0
Company Profile
Website:
www.sartorius.com
Citations (36)
(1)
PARP Inhibitors Effectively Reduce MAPK Inhibitor Resistant Melanoma Cell Growth and Synergize with MAPK Inhibitors through a Synthetic Lethal Interaction In Vitro and In Vivo
Cancer Research Communications
September 5, 2023
Lisa Marie Fröhlich, Heike Niessner, Birgit Sauer, Sofie Kämereit, Eftychia Chatziioannou, Simon Riel, Tobias Sinnberg, Birgit Schittek
data.
Apoptosis Assay
Apoptosis assay
was performed with the
Incucyte
Caspase-3/7 Dye for apoptosis (
Sartorius
4440
) according to the manufacturer's protocol. Briefly, 5 to 10 × 103 cells were seeded into 96-well plate
(2)
A target discovery pipeline identified ILT3 as a target for immunotherapy of multiple myeloma
Cell reports. Medicine
July 18, 2023
Di Meo F, Iyer A, Akama K, Cheng R, Yu C, Cesarano A, Kurihara N, Tenshin H, Aljoufi A, Marino S,
et al
.
IS,
Miltenyi
,
130-097-743
CellTrace™ CFSE Cell Proliferation Kit,
Thermo Fisher
,
C34554
Caspase 3
/7 Green Reagent,
Sartorius
,
4440
magnetic beads,
Miltenyi
,
130-051-301
,, Recombinant DNA,Recombinant DNA,Recombinant DNA ,, pCW-Cas9,Addgene,50661 pLX-sgRNA,Addgene,50662 Nuclight
(3)
Antibody-dependent cellular cytotoxicity-inducing antibodies enhance the natural killer cell anti-cancer response against patient-derived pancreatic cancer organoids
Frontiers in Immunology
July 14, 2023
Nicky A. Beelen, Merel R. Aberle, Virginia Bruno, Steven W. M. Olde Damink, Gerard M. J. Bos, Sander S. Rensen, Lotte Wieten
to reach a final concentration of 10%. To quantify apoptosis, Caspase-3/7 dye (
Sartorius
, Cat. No.
4440
) was added to each well at a final concentration of 2.5 µM. The
NK cells
and Caspase-3/7 dye were added
(4)
Alanine supplementation exploits glutamine dependency induced by SMARCA4/2-loss
Nature Communications
May 20, 2023
Xianbing Zhu, Zheng Fu, Shary Y. Chen, Dionzie Ong, Giulio Aceto, Rebecca Ho, Jutta Steinberger, Anie Monast, Virginie Pilon, Eunice Li,
et al.
Dimethyl-α-ketoglutarate (DMKG, #349631) was from Sigma (Oakville, ON, Canada). Caspase3/7 green dye (
4440
) was from
Sartorius
(Goettingen, Germany). Antibodies against HSP90 (H-114,
1:1
,0000) and β-Actin (Cat#
(5)
Large libraries of single-chain trimer peptide-MHCs enable antigen-specific CD8+ T cell discovery and analysis
Communications Biology
May 16, 2023
William Chour, Jongchan Choi, Jingyi Xie, Mary E. Chaffee, Thomas M. Schmitt, Kathryn Finton, Diana C. DeLucia, Alexander M. Xu, Yapeng Su, Daniel G. Chen,
et al.
microlitres of CTL media containing CD28 antibody (100 ng/mL) and Caspase-3/7 dye (5 µM,
Sartorius
,
4440
) was added into the well of a 96-well plate. 50 k of peptide-pulsed T2 target cells and unlabeled 100
(6)
TSPO acts as an immune resistance gene involved in the T cell mediated immune control of glioblastoma
Acta neuropathologica communications
May 8, 2023
Menevse AN, Ammer LM, Vollmann-Zwerenz A, Kupczyk M, Lorenz J, Weidner L, Hussein A, Sax J, Mühlbauer J, Heuschneider N,
et al
.
(PeproTech, #
310–04
). Afterwards,
Incucyte
®
Caspase-3
/7 Green Dye was added to cells (
1:1000
,
Sartorius
, #
4440
). Cells were imaged for the indicated time points at a 10x magnification.
Tumor
cell apoptosis was quantified
(7)
Inhibition of PLK1 Destabilizes EGFR and Sensitizes EGFR-Mutated Lung Cancer Cells to Small Molecule Inhibitor Osimertinib
Cancers (Basel)
May 2, 2023
Carolien Eggermont, Gustavo J. Gutierrez, Jacques De Grève, Philippe Giron
with the compounds, the
Incucyte
® Annexin V (
4641
,
Sartorius
, Göttingen, Germany) and Caspase-3/7 (
4440
,
Sartorius
) dyes were added to the cells according to the manufacturer’s protocol. To monitor Caspase-3/7
(8)
CDK4/6 inhibition enhances SHP2 inhibitor efficacy and is dependent upon restoration of RB function in malignant peripheral nerve sheath tumors
bioRxiv : the preprint server for biology
February 3, 2023
Wang J, Calizo A, Zhang L, Pino JC, Lyu Y, Pollard K, Zhang X, Larsson AT, Conniff E, Llosa N,
et al
.
96-well plates and treated with the indicated dose of drugs or caspase-3/7 dye for apoptosis (
Sartorius
, #
4440
). Cell proliferation and apoptosis were monitored by a microscope gantry that was connected to a network
(9)
INBRX-120, a CD8α-targeted detuned IL-2 that selectively expands and activates tumoricidal effector cells for safe and durable in vivo responses
Journal for ImmunoTherapy of Cancer
January 18, 2023
Florian J Sulzmaier, Nadja Kern, Sae Jeong Ahn, Anya Polovina, Jason Ho, Abrahim Hussain, Garrett Cyprus, Chelsie Macedo, Rajay Pandit, William Crago,
et al.
T cells to a defined effector-to-target ratio (optimal 10:1).
Incucyte
Caspase-3/7 dye (
Sartorius
, #
4440
) was added to the
co-cultures
to determine target cell apoptosis induced by
NK cells
or CD8 T cells.
(10)
BCL2L11 Induction Mediates Sensitivity to Src and MEK1/2 Inhibition in Thyroid Cancer
Cancers
January 6, 2023
Rose MM, Espinoza VL, Hoff KJ, Pike LA, Sharma V, Hofmann MC, Tan AC, Pozdeyev N, Schweppe RE.
with 50 nM dasatinib, 100 nM trametinib, alone or in combination, and Caspase-3/7 Dye (
Sartorius
Cat.
4440
, Bohemia, NY, USA) for apoptosis was added for a final concentration of 5 μM. Apoptosis was measured
(11)
Ribonuclease 1 Enhances Antitumor Immunity against Breast Cancer by Boosting T cell Activation
International journal of biological sciences
January 1, 2023
Wang YN, Lee HH, Jiang Z, Chan LC, Hortobagyi GN, Yu D, Hung MC.
or primary
T cells
at a
1:10
ratio containing caspase 3/7 substrate green dye (
1:1000
;
Sartorius
, #
4440
), in the presence or absence of
RNase1
treatment. Green fluorescent signals and phase object confluence
(12)
Effects of osteoblast-derived extracellular vesicles on aggressiveness, redox status and mitochondrial bioenergetics of MNNG/HOS osteosarcoma cells
Frontiers in Oncology
December 5, 2022
Marco Ponzetti, Argia Ucci, Chiara Puri, Luca Giacchi, Irene Flati, Daria Capece, Francesca Zazzeroni, Alfredo Cappariello, Nadia Rucci, Stefano Falone
plates and starved O/N. Then, cells were stained with a caspase 3/7-sensitive probe (
Sartorius
, cat. no.
4440
) that crosses
cell membranes
but is only fluorescent in cells with active caspases 3/7, along with OB-EVs
(13)
Large libraries of single-chain trimer peptide-MHCs enable rapid antigen-specific CD8+ T cell discovery and analysis
Research square
November 18, 2022
Heath J, Chour W, Choi J, Xie J, Chaffee M, Schmitt T, Finton K, Delucia D, Xu A, Su Y,
et al
.
peptide. 100 μL of CTL media containing CD28 antibody (100 ng/mL) and Caspase-3/7 dye (5 μM,
Sartorius
,
4440
) was added into the well of a 96-well plate. 50 k of peptide pulsed T2 target cells and unlabeled 100
(14)
Extensive preclinical validation of combined RMC-4550 and LY3214996 supports clinical investigation for KRAS mutant pancreatic cancer
Cell Reports Medicine
November 15, 2022
Katrin J. Frank, Antonio Mulero-Sánchez, Alexandra Berninger, Laura Ruiz-Cañas, Astrid Bosma, Kıvanç Görgülü, Nan Wu, Kalliope N. Diakopoulos, Ezgi Kaya-Aksoy, Dietrich A. Ruess,
et al.
commercial
assays
,, CellTiter-Glo 3D,Promega,REF G9682LOT 0000496328 Caspase 3/7 reagent,
Sartorius
,#
4440
,, Deposited data,Deposited data,Deposited data ,, Uncropped
Western Blots
,Mendeley,Mendeley Data:
(15)
NDUFS3 knockout cancer cells and molecular docking reveal specificity and mode of action of anti-cancer respiratory complex I inhibitors
Open Biology
November 9, 2022
Ivana Kurelac, Beatrice Cavina, Manuela Sollazzo, Stefano Miglietta, Agnese Fornasa, Monica De Luise, Maria Iorio, Eleonora Lama, Daniele Traversa, Hamid Razi Nasiri,
et al.
evaluation Apoptosis upon drug treatments was determined using the
Incucyte
Caspase-3/7 green dye (
Sartorius
#
4440
). Cells (SKOV3, HCT116 and B16-F10) were seeded in a 96-wells plate in high glucose DMEM, as described
(16)
Computational pharmacogenomic screen identifies drugs that potentiate the anti-breast cancer activity of statins
Nature communications
October 24, 2022
van Leeuwen JE, Ba-Alawi W, Branchard E, Cruickshank J, Schormann W, Longo J, Silvester J, Gross PL, Andrews DW, Cescon DW,
et al
.
DR51000
), 10 µM TMRE (
Life Technologies
,
T669
) and 5 µM
IncuCyte
®
Caspase-3
/7 Dye for Apoptosis (
Sartorius
,
4440
). DRAQ5
staining
was used to identify and segment individual cells as well as to
measure
nuclear condensation,
(17)
VRK1 as a synthetic lethal target in VRK2 promoter–methylated cancers of the nervous system
JCI Insight
October 10, 2022
Jonathan So, Nathaniel W. Mabe, Bernhard Englinger, Kin-Hoe Chow, Sydney M. Moyer, Smitha Yerrum, Maria C. Trissal, Joana G. Graca Marques, Jason J. Kwon, Brian H Shim,
et al.
catalog 353047). Then 5 mM of
IncuCyte
Caspase-3
/7 Green
Apoptosis Assay
Reagent (
Sartorius
, catalog
4440
) as well as
1:500
of Nuclight Rapid Red Dye (Sartorius, catalog 4717) were added to each well. The plate
(18)
SMAD2/3 mediate oncogenic effects of TGF-β in the absence of SMAD4
Communications biology
October 7, 2022
Bertrand-Chapel A, Caligaris C, Fenouil T, Savary C, Aires S, Martel S, Huchedé P, Chassot C, Chauvet V, Cardot-Ruffino V,
et al
.
serum starved (1% FBS medium), treated or not with TGF-β and cleaved-Caspase 3/7 Green dye (
Sartorius
#
4440
) was added in the culture medium to follow apoptosis. Images were acquired and analyzed for 72 h with
(19)
Multifaceted role of RNA editing in promoting loss-of-function of PODXL in cancer
iScience
August 19, 2022
Ting Fu, Tracey W. Chan, Jae Hoon Bahn, Tae-Hyung Kim, Amy C. Rowat, Xinshu Xiao
,
Thermo Fisher Scientific
,Cat#
V35004
"
Incucyte
®
Caspase 3
/7 Green Dye for apoptosis, Green",
Sartorius
,Cat#
4440
AgeI-HF
,
NEB
,Cat#
R3552S
EcoRI-HF
,
NEB
,Cat#
R3101S
NotI-HF
,
NEB
,Cat#
R3189S
BstBI
,
NEB
,Cat#
R0519S
XbaI
,
NEB
,Cat#
(20)
Targeting MDM4 as a Novel Therapeutic Approach in Prostate Cancer Independent of p53 Status
Cancers (Basel)
August 16, 2022
Javier Octavio Mejía-Hernández et al.
iodide [PI] (5 μg/mL/well; Cat. P4864, Sigma-Aldrich),
Incucyte
®
Caspase-3
/7 Red Dye (
1:1000
/well, Cat.
4440
,
Sartorius
, New York, NY, USA) and combinations of these. The concentration of DMSO (vehicle) (Cat.
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Incucyte® Caspase-3/7 Green Reagent from Sartorius
Supplier Page
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Product Specs
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