dam-/dcm- Competent E. coli from New England Biolabs

Product Specs
Itemdam-/dcm- Competent E. coli
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberC2925H
Quantity20 x 0.05 ml
Genotypeara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10) TetS endA1 rspL136 (StrR) dam13::Tn9 (CamR) xylA-5 mtl-1 thi-1 mcrB1 hsdR2
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
Cell Type (standardized)Competent Cells
Validated Species ReactivityEscherichia
SpeciesE. coli
Temperature-80 C
Cell TypeCompetent Cells

Specifications/Features

Transformation Efficiency:
> 2 x 10⁶ cfu/µg

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Genetic evidence for the interaction between Bacillus anthracis-encoded phage receptors and their cognate phage-encoded receptor binding proteinsFrontiers in MicrobiologyOctober 31, 2023Samantha Forrest, Sarah Ton, Samantha L. Sholes, Sarah Harrison, Roger D. Plaut, Kathleen Verratti, Michael Wittekind, Elham Ettehadieh, Bryan Necciai, Shanmuga Sozhamannan, et al.Sciences (Burlington, MA). Constructs were then transformed into competent dam−/dcm− E. coli cells (NEB C2925H) and purified to yield unmethylated plasmid stocks. 2.10. Electroporation of plasmids into Bacillus anthracis

(2) Tailoring H2O2 generation kinetics with magnesium alloys for efficient disinfection on titanium surfaceScientific ReportsDecember 1, 2020Jimin Park et al.bacteria and test of antibacterial activity Bacterial cultures of dam−/dcm− Escherichia coli (NEB, C2925H) were chosen as test samples, respectively, for the antibacterial activity experiments. The bacteria

(3) Cell cycle dynamics of lamina‐associated DNA EMBO reportsNovember 5, 2020Tom van Schaik, Mabel Vos, Daan Peric‐Hupkes, Patrick HN Celie, Bas van Steenselassayed as follows. Unmethylated control plasmid was produced in dam − bacteria (New England BioLabs #C2925H), and methylation status was confirmed by DpnI and DpnII digestion. A concentration range (0.125, 0.5,

(4) Target-dependent nickase activities of the CRISPR–Cas nucleases Cpf1 and Cas9Nature MicrobiologyMay 1, 2019Becky Xu Hua Hua Fu, Justin D. Smith, Ryan T. Fuchs, Megumu Mabuchi, Jennifer Curcuru, G. Brett Robb, Andrew Z. FireC-terminal SV40 NLS. Recombinant proteins were expressed in modified E. coli NiCo21 (DE3) cells (NEB C2925H) harboring the Cpf1 expression plasmid by growing in LB at 23°C for 16 hr in presence of IPTG at 0.4mM.

(5) The ATRX cDNA is prone to bacterial IS10 element insertions that alter its structureSpringerPlusDecember 1, 2014David Valle-García, Lyra M Griffiths, Michael A Dyer, Emily Bernstein, Félix Recillas-Targaa second ligation reaction. Ligation product was transformed into dam-/dcm- competent E. coli (NEB C2925H). Colonies were mini-prepped and sequenced. ATRX PCR analysis and sequencing Transfections PCR analyses

(6) DNA delivery in adult mouse eyes: An update with corneal endothelium outcomesMethods in Molecular BiologyJune 3, 2014John M. Nickerson, Shannon E. Getz, Jana T. Sellers, Micah A. Chrenek, Penny Goodman, Christiana J. Bernal, Jeffrey H. Boatrightresistance gene for selection and growth (18). Plasmid was isolated from transformed DAM−/DCM− cells (C2925H; New England Biolabs) after overnight growth in Luria broth using a GIGAprep kit (Qiagen) following

(7) Rapid targeted gene disruption in Bacillus anthracisBMC BiotechnologyDecember 1, 2013Roland J Saldanha, Adin Pemberton, Patrick Shiflett, Jiri Perutka, Jacob T Whitt, Andrew Ellington, Alan M Lambowitz, Ryan Kramer, Deborah Taylor, Thomas J Lamkininto Bacillus anthracis was prepared from a dam methylase deficient host E. coli (New England Biolabs C2925H) with genotype: ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10)

(8) Dihydroxyacetone metabolism in Haloferax volcaniiFrontiers in MicrobiologyNovember 1, 2013Matthew Ouellette, Andrea M. Makkay, R. Thane Papkeplasmids (listed in Table 1) were subcloned in dam−/dcm− Competent E. coli (New England BioLabs, Cat. # C2925H) to produce demethylated plasmids for transformation of Hfx. volcanii. Hfx. volcanii H26 colonies were

(9) Subretinal Delivery and Electroporation in Pigmented and Nonpigmented Adult Mouse EyesRetinal DevelopmentJuly 9, 2013John M. Nickerson, Penny Goodman, Micah A. Chrenek, Christiana J. Johnson, Lennart Berglin, T. Michael. Redmond, Jeffrey H. BoatrightCancer Institute, Amsterdam, The Netherlands. Plasmid was isolated from transformed DAM−/DCM− cells (catalog number C2925H) from New England Biolabs. Escherichia coli are grown overnight in Luria broth using a Qiagen Endotoxin

(10) Design of a single plasmid-based modified yeast one-hybrid system for investigation of in vivo protein-protein and protein-DNA interactionsBioTechniquesFebruary 9, 2009Gang Chen, Lisa M. DenBoer, Jumi A. ShinUSA). Bacterial and Yeast Strains Escherichia coli DH5α (Stratagene, La Jolla, CA, USA) or dam−/dcm− C2925H (New England Biolabs) was used for standard cloning and for rescue of plasmids from yeast cells. Saccharomyces cerevisiae

(11) Development of a broad-host-range sacB-based vector for unmarked allelic exchangeBMC Research NotesFebruary 26, 2008Christopher J MarxNdeI and SacII. The resulting plasmid, pCM432, was then transformed into the dam dcm E. coli strain, C2925H (ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10) TcSendA1

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(1)

7/16/2015Competent Cells Without Plasmid Methylation Problems J GI use these competent cells when I need to produce DNA plasmid that is free of dam or dcm methylation. This is important when I need to perform restriction digests on plasmid sequences that would have... Read Review