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Products
BlpI (Bpu1102I)
BlpI
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BlpI
BlpI from New England Biolabs
Be the first to write a review!
Citations:
(12)
Supplier Page
Supplier Page from
New England Biolabs for
BlpI
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
BlpI
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0585S
Concentration
10 u/µL
Quantity
500 units
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
GC/TNAGC
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (12)
(1)
T-REX17 is a transiently expressed non-coding RNA essential for human endoderm formation
eLife
January 31, 2023
Landshammer A, Bolondi A, Kretzmer H, Much C, Buschow R, Rose A, Wu HJ, Mackowiak SD, Braendl B, Giesselmann P,
et al
.
et al., 2014) was digested with
BstXI
(
New England Biolabs
,
R0113S
) and
BlpI
(
New England Biolabs
,
R0585S
) and the linearized plasmid was gel extracted with the QIAquick Gel Extraction Kit (Quiagen, 28704).
(2)
A dual MTOR/NAD+ acting gerotherapy
bioRxiv
January 22, 2023
Jinmei Li, Sandeep Kumar, Kirill Miachin, Nicholas L. Bean, Ornella Halawi, Scott Lee, JiWoong Park, Tanya H. Pierre, Jin-Hui Hor, Shi-Yan Ng,
et al.
from
Promega
;
NEBNext Ultra II Q5 Master Mix
(cat. #
M0544L
),
BstXI
(cat. #
R0113L
), Blp I (cat. #
R0585L
) from
New England Biolabs
;
NucleoSpin Blood
DNA isolation kit (cat. # 740950.50) from
Macherey Nagel
;
(3)
Myristoylated Neuronal Calcium Sensor-1 captures the ciliary vesicle at distal appendages
bioRxiv : the preprint server for biology
January 10, 2023
Kanie T, Ng R, Abbott KL, Pongs O, Jackson PK.
oligonucleotides encoding sgRNAs into 25 ng of the pMCB306 vector digested with
BstXI
(
R0113S
,
NEB
) and
BlpI
(
R0585S
,
NEB
) restriction enzymes along with 0.25 μl of T4 ligase (
M0202S
,
NEB
) in 2.5 μl total reaction volume.
(4)
A hierarchical pathway for assembly of the distal appendages that organize primary cilia
bioRxiv : the preprint server for biology
January 10, 2023
Kanie T, Love JF, Fisher SD, Gustavsson AK, Jackson PK.
oligonucleotides encoding sgRNAs into the pMCB306 vector digested with
BstXI
(
R0113S
,
NEB
) and
BlpI
(
R0585S
,
NEB
) restriction enzymes. Before
ligation
, 4 µM of forward and reverse oligonucleotides listed in “Source
(5)
Generation of dual-gRNA library for combinatorial CRISPR screening of synthetic lethal gene pairs
STAR Protocols
September 16, 2022
Shan Tang, Xue Wu, Jinghui Liu, Qiongsi Zhang, Xinyi Wang, Shuai Shao, Birkan Gokbag, Kunjie Fan, Xiaoqi Liu, Fuhai Li,
et al.
(FBS),VWR/Avantor,97068-091 EZ-VISION Blue-Light DNA Dye,VWR,10791-798
XbaI
,
New England Biolabs
,
R0145S
BlpI
,
New England Biolabs
,
R0585S
BsmBI-v2
,
New England Biolabs
,
R0739S
X-tremeGENE™ 9 DNA Transfection Reagent
,
Roche
/Sigma,6365787001 ,, Critical
(6)
Cannabinoid receptor 1 antagonist genistein attenuates marijuana-induced vascular inflammation
Cell
May 12, 2022
Tzu-Tang Wei et al.
,
Cayman Chemical Company
,
13884
Oil Red O
,
Sigma-Aldrich
,O0625 BstXI,
Thermo Fisher Scientific
,
FD1024
BlpI
,
NEB
,
R0585S
DNA ligation kit,Takara Bio,6023 Lipofectamine 2000,
Thermo Fisher Scientific
,
11668019
CellTiter-Glo 2.0 Assay
(7)
Epigenetic inheritance is gated by naïve pluripotency and Dppa2
The EMBO journal
April 4, 2022
Carlini V, Policarpi C, Hackett JA.
T4‐DNA ligase
(
NEB
#
M0202S
) for 1 h at 37°C into pPB_U6::gRNA_EF1a::BFP‐Puro digested with
BlpI
(
NEB
#
R0585S
) and
BstXI
(
NEB
#
R0113S
). All ligated assembled plasmids were amplified by
bacteria
transformation and
(8)
A CRISPR/Cas9-Engineered
ARID1A
-Deficient Human Gastric Cancer Organoid Model Reveals Essential and Nonessential Modes of Oncogenic Transformation
Cancer Discovery
June 1, 2021
Yuan-Hung Lo et al.
The sgRNA vector was digested by
BstXI
(
New England BioLabs
,
R0113
) and
BlpI
(
New England BioLabs
,
R0585
) at 37°C for 6 hours. The linearized vectors were separated on a 1% agarose gel. Linearized vectors
(9)
Nutrient status shapes selfish mitochondrial genome dynamics across different levels of selection
eLife
September 22, 2020
Bryan L Gitschlag, Ann T Tate, Maulik R Patel
∆mtDNA frequency "Peptide, recombinant protein",
BlpI
restriction endonuclease,
New England Biolabs
,Cat#
R0585L
,See Materials and methods:
Genetic crosses
and
genotyping
"Chemical compound, drug",Isopropyl-β-D-thiogalactopyranoside,Research
(10)
Thrombospondin-1 Mediates Axon Regeneration in Retinal Ganglion Cells
Neuron
August 21, 2019
Bray ER, Yungher BJ, Levay K, Ribeiro M, Dvoryanchikov G, Ayupe AC, Thakor K, Marks V, Randolph M, Danzi MC,
et al
.
(
Bio-Rad
).
Cloning
: Enzymes used:
AgeI
(
NEB
, R3552),
BamHI
(
NEB
,
R3136
),
BglII
(
NEB
, R0144),
BlpI
(
NEB
,
R0585
),
BspEI
(
NEB
,
R0540
)
EcoRI
(
NEB
,
R3101
),
HindIII
(
NEB
, R3104),
KpnI
(
NEB
, R3142),
MfeI
(
NEB
, R3589),
(11)
Bacterial expression, correct membrane targeting and functional folding of the HIV-1 membrane protein Vpu using a periplasmic signal peptide
PLOS ONE
February 22, 2017
Arpan Deb et al.
intermediate vector by digestion with
NcoI
(
NEB
; cat#
R3193S
) and
BlpI
restriction enzymes (
NEB
; cat#
R0585S
). The same pair of restriction enzymes were also used to linearize the pET26(b) vector by Novagen. The
(12)
Tighter αC-helix–αL16-helix interactions seem to make p38α less prone to activation by autophosphorylation than Hog1
Bioscience Reports
April 1, 2016
Masha Tesker, Sadiduddin Edbe Selamat, Jonah Beenstock, Ruchama Hayouka, Oded Livnah, David Engelberg
Then, the DNA was cloned to the vector of interest, using the
BstEII
(
NEB
#
R3162S
) and
BlpI
(
NEB
#
R0585S
) restriction enzymes for HOG1 and the
EcoRI
(
NEB
#
R3101S
) restriction enzyme for p38α. All HOG1 molecules
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BlpI from New England Biolabs
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