BlpI from New England Biolabs

Product Specs
ItemBlpI
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR0585L
Quantity2500 units
Concentration10 u/µL
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-20 C

Sequence

GC/TNAGC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) T-REX17 is a transiently expressed non-coding RNA essential for human endoderm formationeLifeJanuary 31, 2023Landshammer A, Bolondi A, Kretzmer H, Much C, Buschow R, Rose A, Wu HJ, Mackowiak SD, Braendl B, Giesselmann P, et al.et al., 2014) was digested with BstXI (New England Biolabs, R0113S) and BlpI (New England Biolabs, R0585S) and the linearized plasmid was gel extracted with the QIAquick Gel Extraction Kit (Quiagen, 28704).

(2) A dual MTOR/NAD+ acting gerotherapybioRxivJanuary 22, 2023Jinmei Li, Sandeep Kumar, Kirill Miachin, Nicholas L. Bean, Ornella Halawi, Scott Lee, JiWoong Park, Tanya H. Pierre, Jin-Hui Hor, Shi-Yan Ng, et al.from Promega; NEBNext Ultra II Q5 Master Mix (cat. # M0544L), BstXI (cat. # R0113L), Blp I (cat. # R0585L) from New England Biolabs; NucleoSpin Blood DNA isolation kit (cat. # 740950.50) from Macherey Nagel;

(3) Myristoylated Neuronal Calcium Sensor-1 captures the ciliary vesicle at distal appendagesbioRxiv : the preprint server for biologyJanuary 10, 2023Kanie T, Ng R, Abbott KL, Pongs O, Jackson PK.oligonucleotides encoding sgRNAs into 25 ng of the pMCB306 vector digested with BstXI (R0113S, NEB) and BlpI (R0585S, NEB) restriction enzymes along with 0.25 μl of T4 ligase (M0202S, NEB) in 2.5 μl total reaction volume.

(4) A hierarchical pathway for assembly of the distal appendages that organize primary ciliabioRxiv : the preprint server for biologyJanuary 10, 2023Kanie T, Love JF, Fisher SD, Gustavsson AK, Jackson PK.oligonucleotides encoding sgRNAs into the pMCB306 vector digested with BstXI (R0113S, NEB) and BlpI (R0585S, NEB) restriction enzymes. Before ligation, 4 µM of forward and reverse oligonucleotides listed in “Source

(5) Generation of dual-gRNA library for combinatorial CRISPR screening of synthetic lethal gene pairsSTAR ProtocolsSeptember 16, 2022Shan Tang, Xue Wu, Jinghui Liu, Qiongsi Zhang, Xinyi Wang, Shuai Shao, Birkan Gokbag, Kunjie Fan, Xiaoqi Liu, Fuhai Li, et al.(FBS),VWR/Avantor,97068-091 EZ-VISION Blue-Light DNA Dye,VWR,10791-798 XbaI,New England Biolabs,R0145S BlpI,New England Biolabs,R0585S BsmBI-v2,New England Biolabs,R0739S X-tremeGENE™ 9 DNA Transfection Reagent,Roche/Sigma,6365787001 ,, Critical

(6) Cannabinoid receptor 1 antagonist genistein attenuates marijuana-induced vascular inflammationCellMay 12, 2022Tzu-Tang Wei et al.,Cayman Chemical Company,13884 Oil Red O,Sigma-Aldrich,O0625 BstXI,Thermo Fisher Scientific,FD1024 BlpI,NEB,R0585S DNA ligation kit,Takara Bio,6023 Lipofectamine 2000,Thermo Fisher Scientific,11668019 CellTiter-Glo 2.0 Assay

(7) Epigenetic inheritance is gated by naïve pluripotency and Dppa2The EMBO journalApril 4, 2022Carlini V, Policarpi C, Hackett JA.T4‐DNA ligase (NEB #M0202S) for 1 h at 37°C into pPB_U6::gRNA_EF1a::BFP‐Puro digested with BlpI (NEB #R0585S) and BstXI (NEB #R0113S). All ligated assembled plasmids were amplified by bacteria transformation and

(8) A CRISPR/Cas9-Engineered ARID1A-Deficient Human Gastric Cancer Organoid Model Reveals Essential and Nonessential Modes of Oncogenic TransformationCancer DiscoveryJune 1, 2021Yuan-Hung Lo et al.The sgRNA vector was digested by BstXI (New England BioLabs, R0113) and BlpI (New England BioLabs, R0585) at 37°C for 6 hours. The linearized vectors were separated on a 1% agarose gel. Linearized vectors

(9) Nutrient status shapes selfish mitochondrial genome dynamics across different levels of selectioneLifeSeptember 22, 2020Bryan L Gitschlag, Ann T Tate, Maulik R Patel∆mtDNA frequency "Peptide, recombinant protein",BlpI restriction endonuclease,New England Biolabs,Cat#R0585L,See Materials and methods: Genetic crosses and genotyping "Chemical compound, drug",Isopropyl-β-D-thiogalactopyranoside,Research

(10) Thrombospondin-1 Mediates Axon Regeneration in Retinal Ganglion CellsNeuronAugust 21, 2019Bray ER, Yungher BJ, Levay K, Ribeiro M, Dvoryanchikov G, Ayupe AC, Thakor K, Marks V, Randolph M, Danzi MC, et al.(Bio-Rad). Cloning: Enzymes used: AgeI (NEB, R3552), BamHI (NEB, R3136), BglII (NEB, R0144), BlpI (NEB, R0585), BspEI (NEB, R0540) EcoRI (NEB, R3101), HindIII (NEB, R3104), KpnI (NEB, R3142), MfeI (NEB, R3589),

(11) Bacterial expression, correct membrane targeting and functional folding of the HIV-1 membrane protein Vpu using a periplasmic signal peptidePLOS ONEFebruary 22, 2017Arpan Deb et al.intermediate vector by digestion with NcoI (NEB; cat# R3193S) and BlpI restriction enzymes (NEB; cat# R0585S). The same pair of restriction enzymes were also used to linearize the pET26(b) vector by Novagen. The

(12) Tighter αC-helix–αL16-helix interactions seem to make p38α less prone to activation by autophosphorylation than Hog1Bioscience ReportsApril 1, 2016Masha Tesker, Sadiduddin Edbe Selamat, Jonah Beenstock, Ruchama Hayouka, Oded Livnah, David EngelbergThen, the DNA was cloned to the vector of interest, using the BstEII (NEB # R3162S) and BlpI (NEB # R0585S) restriction enzymes for HOG1 and the EcoRI (NEB #R3101S) restriction enzyme for p38α. All HOG1 molecules

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BlpI

BlpI from New England Biolabs

Sequence

New England Biolabs

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BlpI from New England Biolabs

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