Protein A Agarose (High Affinity) ab193255 from Abcam

Product Specs
ItemProtein A Agarose (High Affinity)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab193255
Quantity1 ML, 5 ML, 25 ML, 100 ML
Product TypeResins
ModeAntibody Purification
Sample TypeCell culture supernatant, Serum, Cell culture media, Ascites Fluid
ApplicationsImmunoprecipitation, Purification, Immunoprecipitation, Purification, Immunoprecipitation, Purification, Immunoprecipitation, Purification

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Protocol to establish an oviduct epithelial cell line derived from Gallus gallus using Percoll for in vitro validation of recombinant proteinsSTAR ProtocAugust 4, 2023Chiron Loubser, Natalya V. NikitinaMax,Polysciences,Cat#24765 OptiPRO SFM,Thermo Fisher Scientific,Cat#12309019 Protein A Agarose,Abcam,Cat#ab193255 β-estradiol,Merck,Cat#E2758 ,, Experimental models: Organisms/strains,Experimental models: Organisms/strains,Experimental models

(2) A mass spectrometry-based approach for the identification of Kpnβ1 binding partners in cancer cellsScientific reportsNovember 23, 2022Okpara MO, Hermann C, van der Watt PJ, Garnett S, Blackburn JM, Leaner VD.against any known antigen was included, together with protein A agarose beads (Abcam, high affinity beads ab193255), to account for proteins non-specifically binding the antibody or beads. For the co-immunoprecipitation

(3) The CtIP-CtBP1/2-HDAC1-AP1 transcriptional complex is required for the transrepression of DNA damage modulators in the pathogenesis of osteosarcomaTranslational OncologyJuly 1, 2022Xun Chen, Qian Zhang, Xiaoqian Dang, Jinzhu Fan, Tao Song, Zhong Li, Ning Duan, Wentao Zhangmin at 4 °C, followed by IP procedures using anti-CtIP- and IgG-associated protein A agarose (Abcam; #ab193255). The immunoprecipitated proteins were resolved by sodium dodecyl-sulfate polyacrylamide gel electrophoresis

(4) Both a hypoxia-inducible EYA3 and a histone acetyltransferase p300 function as coactivators of SIX5 to mediate tumorigenesis and cancer progressionAnnals of translational medicineJuly 1, 2022Yang C, Liu H.(Proteintech, Wuhan, Hubei, China; #21196-1-AP) associated protein A agarose (Abcam, Shanghai, China; #ab193255). The immunoprecipitates were rinsed six times with RIPA buffer, followed by denaturation in the sodium

(5) miR‑4735‑3p inhibits cell migration and invasion of gastric cancer by downregulating NEDD9Oncology LettersJune 10, 2022Yanchun Maimmunoprecipitated with RNA-binding proteins was isolated after addition of protein A agarose (catalog no. ab193255; 25 µl) and protein G agarose (catalog no. ab193258; 25 µl) (both from Abcam). Following washes, RNA

(6) S100A11 Promotes Liver Steatosis via FOXO1-Mediated Autophagy and LipogenesisCellular and molecular gastroenterology and hepatologyJanuary 1, 2021Zhang L, Zhang Z, Li C, Zhu T, Gao J, Zhou H, Zheng Y, Chang Q, Wang M, Wu J, et al.then were incubated with protein G agarose (20398; Thermo Fisher Scientific) or protein A agarose (ab193255; Abcam) according to the binding capacity of the primary antibody to protein G or A agarose and rocked

(7) CDE-1 suppresses the production of risiRNA by coupling polyuridylation and degradation of rRNABMC BiologyDecember 1, 2020Yun Wang, Chenchun Weng, Xiangyang Chen, Xufei Zhou, Xinya Huang, Yonghong Yan, Chengming Zhuprotease inhibitors (Thermo)). Immunoprecipitations with anti-FLAG® M2 affinity gel (a2220, Sigma) or agarose beads (ab193255, Abcam) with anti-GFP antibody (ab290, Abcam) and anti-SUSI-1 antibody (lot number 20121105, Abmart)

(8) LncRNA DLX6-AS1 Contributes to Epithelial–Mesenchymal Transition and Cisplatin Resistance in Triple-negative Breast Cancer via Modulating Mir-199b-5p/Paxillin AxisCell TransplantationJuly 21, 2020Chuang Du, Yan Wang, Yingying Zhang, Jianhua Zhang, Linfeng Zhang, Jingruo Lilysed and primary antibodies were added and incubated at 4°C overnight on a rotator. Protein A agarose (ab193255, Abcam) and protein G agarose (ab193258, Abcam) were added in order to isolate the RNA immunoprecipitated

(9) PHA-4/FoxA senses nucleolar stress to regulate lipid accumulation in Caenorhabditis elegansNature CommunicationsDecember 1, 2018Jieyu Wu et al.anti-GFP antibody (ab290, Abcam) and IgG (ab172730, Abcam). Subsequently, 40 μl of protein A beads (ab193255, Abcam) was added to each ChIP sample, followed by washing with FA buffer, FA-500 mM buffer, LiCl buffer,

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Protein A Agarose (High Affinity) from Abcam

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Protein A Agarose (High Affinity) from Abcam

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