Fig 1: Heterogeneous Fibroblast Populations in Injured and Uninjured Neonatal Hearts(A) UMAP representation of different FB sub-populations analyzed.(B) Heatmap showing expression of top enriched genes for each FB sub-population.(C) Percentage of each FB sub-population over total nonmyocytes within each sample.(D–H) UMAP plots showing expression of Pro.FB-enriched gene Hmgb2 (D), FBI-enriched gene Cxcl1 (E), FB2-enriched gene Dlk1 (F), FB3-enriched gene Nov (G), and FB4-enriched gene Fbln5 (H).(I) Heatmap showing relative fold induction (Z score) of Serpinb2, Wnt5a, and Ltbp3 expression at various time points after P1 or P8 MI detected by bulk RNA-seq.(J) EdU incorporation (magenta) and vimentin immunofluorescent staining (green) of NRCF cells treated with 200 ng/mL BSA (negative control), 20 ng/mL recombinant SERPINB2, 100 ng/mL recombinant LTBP3, or 100 ng/mL recombinant WNT5A (positive control), with quantification showing the proportion of EdU-positive cells among vimentin-positive cells (fibroblasts) (n = 4 per each group; ****p < 0.0001). Scale bar, 100 µm.See also Figure S6.