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Blunt/TA Ligase Master Mix
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Blunt/TA Ligase Master Mix
Blunt/TA Ligase Master Mix from New England Biolabs
Be the first to write a review!
Citations:
(41)
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New England Biolabs for
Blunt/TA Ligase Master Mix
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Citations
Reviews
Product Specs
Item
Blunt/TA Ligase Master Mix
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0367S
Quantity
50 reactions
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Product Type
NGS Kits
Workflow Step
Adapter Ligation
Type
Enzymes
Temperature
-20 C
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (41)
(1)
Investigations into SARS-CoV-2 and other coronaviruses on mink farms in France late in the first year of the COVID-19 pandemic
PLoS One
August 25, 2023
Marine Wasniewski, Franck Boué, Céline Richomme, Etienne Simon-Lorière, Sylvie Van der Werf, Flora Donati, Vincent Enouf, Yannick Blanchard, Véronique Beven, Estelle Leperchois,
et al.
ng DNA from each sample. Native barcode adapters NBD04 were ligated in
Blunt/TA Ligase Master Mix
(
M0367S
,
NEB
, USA), and the resulting product was purified using AMPure XP beads before being pooled to produce
(2)
Investigations on SARS-CoV-2 and other coronaviruses in mink farms in France at the end of the first year of COVID-19 pandemic
bioRxiv
July 7, 2023
Marine Wasniewski, Franck Boué, Céline Richomme, Etienne Simon-Lorière, Sylvie Van der Werf, Flora Donati, Vincent Enouf, Yannick Blanchard, Véronique Beven, Estelle Leperchois,
et al.
ng DNA from each sample. Native barcode adapters NBD04 were ligated in
Blunt/TA Ligase Master Mix
(
M0367S
,
NEB
, USA), and resulting product was AMPure XP beads purified before pooling to produce a 54 μl equimass
(3)
Rapid whole genome sequencing methods for RNA viruses
Frontiers in Microbiology
February 23, 2023
Masayasu Misu, Tomoki Yoshikawa, Satoko Sugimoto, Yuki Takamatsu, Takeshi Kurosu, Yukiteru Ouji, Masahide Yoshikawa, Masayuki Shimojima, Hideki Ebihara, Masayuki Saijo
reaction volume of 25 μL containing 400 ng of cDNA, 12.5 μL of
Blunt/TA ligase master mix
(catalog number
M0367S
,
New England BioLabs
), 1.5 μL of Native Barcoding Expansion and H2O. The reaction mixture was incubated
(4)
Nano3P-seq: transcriptome-wide analysis of gene expression and tail dynamics using end-capture nanopore cDNA sequencing
Nature Methods
January 1, 2023
Oguzhan Begik, Gregor Diensthuber, Huanle Liu, Anna Delgado-Tejedor, Cassandra Kontur, Adnan Muhammad Niazi, Eivind Valen, Antonio J. Giraldez, Jean-Denis Beaudoin, John S. Mattick,
et al.
was mixed with 5 µl Adapter Mix (AMX), 22.5 µl Rnase-free water and 50 µl Blunt/TA Ligase Mix (
NEB
,
M0367S
) and incubated in room temperature for 10 min. The reaction was cleaned up using 0.8× AMPure XP beads,
(5)
Metagenomic sequencing for detection and identification of the boxwood blight pathogen Calonectria pseudonaviculata
Scientific Reports
December 1, 2022
Shu Yang, Marcela A. Johnson, Mary Ann Hansen, Elizabeth Bush, Song Li, Boris A. Vinatzer
mixing 22.5 μL of the elute with the
Blunt/TA Ligase Master Mix
(
New England Biolabs
, Inc.; Catalog #
M0367S
) and Native Barcode (ONT; Native Barcoding Expansion Kit EXP-NBD104). Barcoded DNA was cleaned up by
(6)
Systematic generation and imaging of tandem repeats reveal base-pairing properties that promote RNA aggregation
Cell Reports Methods
November 21, 2022
Atagun U. Isiktas, Aziz Eshov, Suzhou Yang, Junjie U. Guo
Monarch DNA Gel Extraction Kit
,
New England Biolabs
,Cat#
T1020S
Blunt/TA Ligase Master Mix
,
New England Biolabs
,
M0367S
Instant Sticky-end Ligase Master Mix
,
New England Biolabs
,Cat#
M0370S
QIAprep Spin Miniprep Kit,
Qiagen
,Cat#
(7)
Characterization of full-length CNBP expanded alleles in myotonic dystrophy type 2 patients by Cas9-mediated enrichment and nanopore sequencing
eLife
August 26, 2022
Massimiliano Alfano, Luca De Antoni, Federica Centofanti, Virginia Veronica Visconti, Simone Maestri, Chiara Degli Esposti, Roberto Massa, Maria Rosaria D'Apice, Giuseppe Novelli, Massimo Delledonne,
et al.
excision (see M&M)" Commercial
assay
or kit,
Blunt/TA Ligase Master Mix
,
New England BioLabs
,Cat. No.:
M0367S
,
T4 DNA ligase
for native barcode
ligation
to dA-tailed ends (see M&M) Commercial
assay
or kit,FLO-MIN106D
(8)
The Chromosome-Scale Reference Genome of Macadamia tetraphylla Provides Insights Into Fatty Acid Biosynthesis
Frontiers in Genetics
February 23, 2022
Yingfeng Niu, Guohua Li, Shubang Ni, Xiyong He, Cheng Zheng, Ziyan Liu, Lidan Gong, Guanghong Kong, Wei Li, Jin Liu
United Kingdom) and a
PCR
-free “native barcoding” kit provided by ONT.
Blunt/TA ligase Master Mix
(
M0367S
,
NEB
) was used to ligate native barcode adapters for 10 min at room temperature. A
1:1
volume of AMPure
(9)
Detection of SARS‐CoV‐2 in two cats during the second wave of the COVID‐19 pandemic in France
Veterinary Medicine and Science
January 1, 2022
Matthieu Fritz, Nicolas Nesi, Solène Denolly, Bertrand Boson, Vincent Legros, Serge G. Rosolen, Alexandra Briend‐Marchal, Meriadeg Ar Gouilh, Eric M. Leroy
samples. End‐prepared DNA was ligated with native barcode adapters NBD04 using
Blunt/TA Ligase Master Mix
(
M0367S
;
NEB
). Following the barcode
ligation
reaction, the DNA was cleaned with AMPure XP beads. The two samples
(10)
Genomic skimming and nanopore sequencing uncover cryptic hybridization in one of world’s most threatened primates
Scientific Reports
December 1, 2021
Joanna Malukiewicz et al.
30 ul of end-prepped DNA with 20 ul SQK-LSK108 adapter Mix, and 50 ul NEB Blunt/TA Master Mix (
NEB
M0367S
). The mix was flicked and then incubated at room temperature for 10 min. The adapter-ligated DNA was
(11)
Infection with SARS‐CoV‐2 variant B.1.1.7 detected in a group of dogs and cats with suspected myocarditis
Veterinary Record
November 4, 2021
Luca Ferasin, Matthieu Fritz, Heidi Ferasin, Pierre Becquart, Sandrine Corbet, Meriadeg Ar Gouilh, Vincent Legros, Eric M. Leroy
End‐prepared amplicons were ligated with native barcode adapters NBD04 using
Blunt/TA Ligase Master Mix
(
M0367S
,
NEB
, USA) and then purified with AMPure XP beads. The two samples were then pooled to produce a 60
(12)
The Genome of the CTG(Ser1) Yeast
Scheffersomyces stipitis
Is Plastic
mBio
October 26, 2021
Samuel Vega-Estévez, Andrew Armitage, Helen J. Bates, Richard J. Harrison, Alessia Buscaino
a
PCR
-free kit with NEBNext End Repair (
E6050S
), NEBNext dA-tailing (
E6053S
), and Blunt T/A ligase (
M0367S
)
New England Biolabs
modules.
Sequencing
was performed on a MiSeq benchtop analyzer (Illumina) using
(13)
Report of One-Year Prospective Surveillance of SARS-CoV-2 in Dogs and Cats in France with Various Exposure Risks: Confirmation of a Low Prevalence of Shedding, Detection and Complete Sequencing of an Alpha Variant in a Cat
Viruses
September 3, 2021
Emilie Krafft et al.
End-prepared amplicons were ligated with native barcode adapters NBD04 using
Blunt/TA Ligase Master Mix
(
M0367S
,
New England Biolabs
, Ipswich, MA, USA) and then purified with AMPure XP beads (1× ratio). The two samples
(14)
Tissue-specific DamID protocol using nanopore sequencing
J Biol Methods
August 27, 2021
Georgina Gómez-Saldivar, Dominique A. Glauser, Peter Meister
samples in one
sequencing
experiment.✓ Flow Cell Priming kit (EXP-FLP001, ONT)✓ NEB Blunt/TA Master Mix (
M0367S
,
NEB
)✓
NEBNext Quick Ligation Module
(
E6056S
,
NEB
)✓ AMPure XP 1.8 beads (A63880, Agencourt)✓ Magnetic
(15)
Light-Inducible Spatio-Temporal Control of TLR4 and NF-κB-Gluc Reporter in Human Pancreatic Cell Line
International Journal of Molecular Sciences
August 26, 2021
Anna Stierschneider, Petra Grünstäudl, Katrin Colleselli, Josef Atzler, Christian T. Klein, Harald Hundsberger, Christoph Wiesner
plasmid) by blunt end
ligation
using
Blunt/TA Ligase Master Mix
(
New England BioLabs
, Frankfurt, Germany;
M0367S
) of the
PCR
products and
transfected
in
Escherichia coli
strain
GCI-5α (GeneCopoeiaTM, 1 Rockville,
(16)
Morphological and transcriptomic alterations in neonatal lamb testes following developmental exposure to low-level environmental chemical mixture.
Environmental Toxicology and Pharmacology
August 1, 2021
Chris S. Elcombe, Ana Monteiro, Mohammad Ghasemzadeh-Hasankolaei, Neil P. Evans, Michelle Bellingham
using a barcode expansion kit (Oxford Nanopore – EXP-NBD104) and Blunt/TA Ligase (
New England Biolabs
–
M0367S
). As the barcoding kit only contains twelve individual barcodes, samples were split into two groupings
(17)
Comparative Analysis of Host-Associated Variation in Phytophthora cactorum
Frontiers in Microbiology
July 2, 2021
Charlotte F. Nellist, Andrew D. Armitage, Helen J. Bates, Maria K. Sobczyk, Matteo Luberti, Laura A. Lewis, Richard J. Harrison
PCR
-free method using NEBNext End Repair (
E6050S
), NEBNext dA-tailing (
E6053S
) and Blunt T/A ligase (
M0367S
)
New England Biolabs
modules. Library insert sizes were 400–600 bp and were sequenced using Illumina
(18)
Direct oligonucleotide sequencing with nanopores
Open Research Europe
May 12, 2021
Sachin Chalapati, Conor A Crosbie, Dixita Limbachiya, Nimesh Pinnamaneni
100 uM concentration. Enzymes and buffers T4 PNK -
NEB
-
M0201S
Blunt/TA Ligase Master Mix
-
NEB
-
M0367S
IDTE - IDT - 11-01-02-02 Page 3/8
Qubit ssDNA Assay Kit
-
ThermoFisher
-
Q10212
Nuclease Free Water
(19)
Efficient Expression of Soluble Recombinant Protein Fused with Core-Streptavidin in Bacterial Strain with T7 Expression System
Methods and protocols
December 1, 2020
Tarar A, Alyami EM, Peng CA.
USA) 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. • High fidelity Phusion polymerase (Cat No.:
M0491S
)
Blunt/TA Ligase Master Mix
(Cat No.:
M0367S
)
T4 DNA ligase
(Cat No.:
M0202S
) Monarch
PCR
& DNA cleanup kit (Cat No.: T1030S) BL21(DE3) competent
(20)
Accurate Detection of HPV Integration Sites in Cervical Cancer Samples Using the Nanopore MinION Sequencer Without Error Correction
Frontiers in Genetics
June 26, 2020
Wenjuan Yang, Ying Liu, Ruyi Dong, Jia Liu, Jidong Lang, Jialiang Yang, Weiwei Wang, Jingjing Li, Bo Meng, Geng Tian
end-prepared DNA was ligated with native barcode NB04 from ONT using the
NEB
Blunt/TA Ligase Master Mix
(
M0367S
;
NEB
), and then incubated at 25°C for 15 min. Following the barcode
ligation
reaction, the DNA was cleaned
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Blunt/TA Ligase Master Mix from New England Biolabs
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