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Bst2.0 DNA Polymerase
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Bst2.0 DNA Polymerase
Bst2.0 DNA Polymerase from New England Biolabs
(1)
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Citations:
(12)
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Supplier Page from
New England Biolabs for
Bst2.0 DNA Polymerase
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Bst2.0 DNA Polymerase
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0537S
Quantity
1600 units
Applications
DNA Analysis
Brand/Trademark
New England Biolabs Inc (NEB)
Type
DNA Polymerase
Temperature
-20 C
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (12)
(1)
Fluoropolymer Coated DNA Nanoclews for Volumetric Visualization of Oligonucleotides Delivery and Near Infrared Light Activated Anti‐Angiogenic Oncotherapy
Advanced Science
November 1, 2023
Peng Zhang, Ranran Guo, Haiting Zhang, Wuli Yang, Ye Tian
heating, the resultant cyclized ssDNA template was hybridized with primer and then Bst 2.0 DNA polymerase (
M0537S
,
New England Biolabs
) was added. RCA was performed at 60 °C for 17 h and then the polymerase was deactivated
(2)
Development of a loop-mediated isothermal amplification (LAMP)-based electrochemical test for rapid detection of SARS-CoV-2
iScience
September 15, 2023
Khushboo Borah Slater, Muhammad Ahmad, Aurore Poirier, Ash Stott, Bianca Sica Siedler, Matthew Brownsword, Jai Mehat, Joanna Urbaniec, Nicolas Locker, Yunlong Zhao,
et al.
dioxyribonucleotides dNTPs,
New England Biolabs
,
N0447S
320U/mL Bst 2.0 DNA polymerase,
New England Biolabs
,
M0537S
"7500U/mL
warmStart® RTx reverse transcriptase
(
NEB
),",
New England Biolabs
,
M0380S
2000U/mL thermostable
(3)
Specific Regulation of m(6)A by SRSF7 Promotes the Progression of Glioblastoma
Genomics, proteomics & bioinformatics
August 1, 2023
Cun Y, An S, Zheng H, Lan J, Chen W, Luo W, Yao C, Li X, Huang X, Sun X,
et al
.
min. Then 0.5 U
SplintR ligase
(Catalog No.
M0375S
,
NEB
), 0.01 U Bst 2.0 DNA polymerase (Catalog No.
M0537S
,
NEB
), and 10 nM ATP (Catalog No.
P0756L
,
NEB
) was added to a final volume of 20 μl and incubated at
(4)
Targeted Demethylation of the TGFβ1 mRNA Promotes Myoblast Proliferation via Activating the SMAD2 Signaling Pathway
Cells
March 24, 2023
Kaiping Deng, Zhipeng Liu, Xiaodan Li, Zhen Zhang, Yixuan Fan, Qunhao Huang, Yanli Zhang, Feng Wang
product was further mixed with 3 µL of enzyme mixture containing 0.01 U Bst 2.0 DNA polymerase (
NEB
, #
M0537S
), 0.5 U
SplintR ligase
(
NEB
, #
M0375S
), and 10 nmol ATP (
NEB
, #
P0756S
), and then were incubated at 40
(5)
N6-methyladenosine RNA modification promotes viral genomic RNA stability and infection
Nature communications
November 2, 2022
Zhang T, Shi C, Hu H, Zhang Z, Wang Z, Chen Z, Feng H, Liu P, Guo J, Lu Q,
et al
.
°C for 1 min, 50 °C for 1 min, and then 40 °C for 6 min. Then, 0.01 U Bst 2.0 DNA polymerase (
NEB
,
M0537S
), 0.5 U
SplintR ligase
(
NEB
,
M0375S
), and 10 nmol ATP were added to the mixture to a final volume of
(6)
Revisiting proboscidean phylogeny and evolution through total evidence and palaeogenetic analyses including Notiomastodon ancient DNA
iScience
January 21, 2022
Sina Baleka, Luciano Varela, P. Sebastián Tambusso, Johanna L.A. Paijmans, Dimila Mothé, Thomas W. Stafford, Richard A. Fariña, Michael Hofreiter
(
afu UDG
),
NEB
,Cat#
M0279S
FastAP,
Thermo Fisher
,Cat#
EF0651
Dynabeads MyOne C1,
Thermo Fisher
,Cat#
65001
Bst 2.0 polymerase,
NEB
,Cat#
M0537S
T4 DNA Polymerase
,
Thermo Fisher
,Cat#
EP0061
Buffer tango (10×),
Thermo Fisher
,Cat#
BY5
T4 DNA ligase,
Thermo Fisher
(7)
N6-methyladenosine RNA modification regulates strawberry fruit ripening in an ABA-dependent manner
Genome Biology
December 1, 2021
Leilei Zhou, Renkun Tang, Xiaojing Li, Shiping Tian, Bingbing Li, Guozheng Qin
min, 60 °C for 1 min, 50 °C for 1 min, and 40 °C for 6 min. Then, 0.01 U Bst 2.0 DNA polymerase (
NEB
,
M0537S
), 0.5 U
SplintR ligase
(
NEB
,
M0375S
), and 10 nmol ATP were added to the mixture to a final volume of
(8)
Epitranscriptomic editing of the RNA N6-methyladenosine modification by dCasRx conjugated methyltransferase and demethylase
Nucleic acids research
July 21, 2021
Xia Z, Tang M, Ma J, Zhang H, Gimple RC, Prager BC, Tang H, Sun C, Liu F, Lin P,
et al
.
Subsequently, 2.5 μl of enzyme mixture containing 0.01 U Bst 2.0 DNA polymerase (
New England Biolabs
, #
M0537S
), 0.5 U
SplintR ligase
(
New England Biolabs
, #
M0375S
) and 10 nmol ATP (
New England Biolabs
, #
P0756S
)
(9)
TRADES: Targeted RNA Demethylation by SunTag System
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
October 1, 2020
Mo J, Chen Z, Qin S, Li S, Liu C, Zhang L, Ran R, Kong Y, Wang F, Liu S,
et al
.
then 40 °C for 6 min. Subsequently, a 3 µL of mixture containing 0.01 U Bst 2.0 DNA polymerase (
NEB
,
M0537s
), 0.5 U
SplintR ligase
(
NEB
,
M0375s
) and 10 nmol ATP (Solarbio, IA0590) were added in the former mixture
(10)
Real-time kinetics and high-resolution melt curves in single-molecule digital LAMP to differentiate and study specific and non-specific amplification
Nucleic Acids Research
April 17, 2020
Justin C Rolando, Erik Jue, Jacob T Barlow, Rustem F Ismagilov
(#
B1003S
), deoxynucleotide solution (#
N0447S
), Bovine Serum Albumen (
BSA
, #B9000S0), Bst 2.0 (8,000 U/ml, #
M0537S
) and Bst 3.0 (8000 U/ml, #
M0374S
) were purchased from
New England Biolabs
(Ipswich, MA, USA). Ambion
(11)
Real-Time, Digital LAMP with Commercial Microfluidic Chips Reveals the Interplay of Efficiency, Speed, and Background Amplification as a Function of Reaction Temperature and Time
Analytical Chemistry
January 2, 2019
Justin C. Rolando, Erik Jue, Nathan G. Schoepp, Rustem F. Ismagilov
LoopF/LoopB, 1 mg/mL
BSA
(New England BioLabs; ref B90005), 320 U/mL Bst 2.0 (
New England BioLabs
; ref
M0537S
), Ambion
RNase cocktail
(ThermoFisher, ref
AM2286
, 5 U/mL
RNase A
, 400 U/mL TNase T1), 2 μM SYTO 9 (ThermoFisher,
(12)
Preliminary Study on The RT-LAMP Assay for Rabbit Hemorrhagic Disease Virus Type 2 Detection
Proceedings of the 2016 International Conference on Biological Engineering and Pharmacy (BEP 2016)
January 1, 2017
Zexiao YANG, Yilin LUO, Zhengqun MENG, Xueping YAO, Han ZHANG, Yin WANG, Xiaoxue XIANG, Lijun ZHOU, Yadong LIU, Yan LI
and RNAiso Plus were provided by the TaKaRa Biotechnology(Dalian) Co., Ltd. Bst 2.0 DNA polymerase (
M0537S
) was provided by Beijing
New England Biolabs
Co., Ltd., RHDV-Sch01, VP60 gene fragments of European
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Reviews
Write a Review
(1)
10/29/2014
Good enzyme, some optimization likely required
Colin Cooper
A loop-mediated isothermal amplification (LAMP) study was conducted to detect the presence of pathogen DNA in food samples. Bst 2.0 from NEB was selected because it is advertised as having ...
Read Review
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Bst2.0 DNA Polymerase from New England Biolabs
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