Fig 1: Involvement of Nkx2-5 in the targeting of ErbB2 by lncENST. (a) Relative protein expression of Nkx2-5 in SMMC-7721 cells subjected to lncENST interference or overexpression. Relative (b) mRNA and (c) protein expression of ErbB2 in SMMC-7721 cells transfected with lnc-INT/OV, with additional Nkx2-5 stimulus in lnc-INT-treated cells. For all, the data are expressed as the mean ± SD, n = 3, *p < 0.05.
Fig 2: Effect of Nkx2-5 on ErbB2 expression in HCC-xenografted tumor tissues. (a) Immunofluorescence staining of ErbB2 and Nkx2-5 (punctate green dots in white rectangles) in tumor tissues of HCC-xenografted mice subjected to RFA and lnc-INT/OV injection. Scale bar = 50 µm, ×100. Relative (b) mRNA and (c) protein expression of ErbB2 in tumor tissues of HCC-xenografted mice subjected to RFA and lnc-INT/OV injection. (d) Bioinformatic analysis based on the Champion Chip Transcription Factor Search Portal from Qiagen revealed the binding site between the transcription factor (TF in the image) Nkx2-5 and the ErbB2 gene. (e) Chromatin immunoprecipitation of the Nkx2-5 protein and the ErbB2 gene. Pull down was performed using an antibody against Nkx2-5. The expression of ErbB2 was detected in SMMC-7721 cells treated with lnc/Nkx-OV/INT. For all, the data are expressed as the mean ± SD, n = 3, *p < 0.05.
Supplier Page from Abcam for Recombinant Human Nkx2.5 protein