SARS-CoV-2 (2019-nCoV) Spike S1(D614G)-His Recombinant Protein, (HPLC-verified) 40591-V08H3 from Sino Biological, Inc.

Product Specs
ItemSARS-CoV-2 (2019-nCoV) Spike S1(D614G)-His Recombinant Protein, (HPLC-verified)
CompanySino Biological, Inc.
Price Supplier Page View Company Product Page
Catalog Number40591-V08H3
TypeRecombinant protein
Molecule NameSpike
SpeciesSARS-CoV-2
FormatLyophilized from sterile PBS,pH 7.4
Conjugate/TagC-His
Source/Expression SystemHEK293 Cells
Molecular WeightThe recombinant SARS-CoV-2 (2019-nCoV) Spike S1(D614G)-His Recombinant Protein consists of 681 amino acids and predicts a molecular mass of 76.41 kDa
Purity> 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
Activity1. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(10108-H05H) at 2 µg/mL (100 µL/well) can bind 2019-nCoV S1(D614G)h(40591-V08H3), the EC50 of 2019-nCoV S1(D614G)h(40591-V08H3) is 250-650 ng/mL. 2. Loaded ACE2 Protein, Human, Recombinant (mFc Tag) (Cat. No. 10108-H05H) on AMC Biosensor, can bind SARS-CoV-2 (2019-nCoV) Spike S1(D614G)-His Recombinant Protein (Cat. No. 40591-V08H3) with an affinity constant of 57.7 nM as determined by Octet RED System

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Sino Biological, Inc.

1400 Liberty Ridge Drive

Suite 101

Wayne, PA 19087

United States

Phone: 215-583-7898
Fax: 267-657-0217

Email: marketing@sinobiological.com

Company Profile

Website: http://www.sinobiological.com/

(1) ACE2-independent SARS-CoV-2 virus entry through cell surface GRP78 on monocytes - evidence from a translational clinical and experimental approachEBioMedicineDecember 1, 2023Han B, Lv Y, Moser D, Zhou X, Woehrle T, Han L, Osterman A, Rudelius M, Choukér A, Lei P.

(2) Exploring the binding effect and mechanism of glycyrrhizin to ovomucin by combining spectroscopic analysis and molecular dockingInternational journal of biological macromoleculesAugust 1, 2023Shengnan Z, Yingjie Z, Junyue C, Shuangshuang S, Xin L, Yuanyuan S.

(3) Multiplexed evaluation of immunity against SARS-CoV-2 variants using surface enhanced fluorescence from a nanostructured plasmonic chipJournal of nanobiotechnologyDecember 15, 2022Hu R, Yang Y, Liu Y, Liao T, Liu Y, Tang J, Wang G, Wang G, Liang Y, Yuan J, et al.

(4) Respiratory mucosal immunity against SARS-CoV-2 after mRNA vaccinationScience immunologyOctober 28, 2022Tang J, Zeng C, Cox TM, Li C, Son YM, Cheon IS, Wu Y, Behl S, Taylor JJ, Chakaraborty R, et al.SARS-CoV-2 proteins including RBD (Sino Biological, 40592-V08H), spike S1 D614G (S1) (Sino Biological, 40591-V08H3), spike S1+S2 ECD (S) (Sino Biological, 40589-V08H4), or nucleocapsid protein (N) (GenScript, Z03488)

(5) Asymptomatic or symptomatic SARS-CoV-2 infection plus vaccination confers increased adaptive immunity to variants of concerniScienceOctober 21, 2022Peifang Sun, Irene Ramos, Camila H. Coelho, Alba Grifoni, Corey A. Balinsky, Sindhu Vangeti, Alison Tarke, Nathaniel I. Bloom, Vihasi Jani, Silvia J. Jakubski, et al.Protein,AcroBiosystems,Cat# SPN-C82Ee Ancestral (WT) S1 D614G SARS-CoV-2 Protein,Sino Biological,Cat# 40591-V08H3 Delta (B.1.617.2) S1 SARS-CoV-2 Protein,Sino Biological,Cat# 40591-V08H23 Omicron (B.1.1.529) S1 SARS-CoV-2

(6) Irradiation of UVC LED at 277 nm inactivates coronaviruses in association to photodegradation of spike proteinHeliyonOctober 17, 2022Qunxiang Ong, J.W. Ronnie Teo, Joshua Dela Cruz, Elijah Wee, Winson Wee, Weiping Hanproteins" EMEM,ATCC,30–2003 RPMI-1640,ATCC,30–2011 Purified recombinant spike proteins,Sinobiological,"40592-V08H80, 40591-V08H3, 40592-V08H9, 40592-V08H27, 40591-V08H, 40607-V08H1" Carboxymethylcellulose,Sigma Aldrich,C4888 Critical

(7) SARS-CoV-2 spike D614G variant confers enhanced replication and transmissibilitybioRxivSeptember 29, 2022Bin Zhou et al.ABclonal. Recombinant SARS-CoV-2 proteins S1–614D and S1–614G with polyhistidine-tag (Cat: 40591-V08H, 40591-V08H3) were purchased from Sino Biological. All proteins were quantitated with Qubit Protein Assay (Thermo

(8) Potent universal beta-coronavirus therapeutic activity mediated by direct respiratory administration of a Spike S2 domain-specific human neutralizing monoclonal antibodyPLoS pathogensJuly 1, 2022Piepenbrink MS, Park JG, Deshpande A, Loos A, Ye C, Basu M, Sarkar S, Khalil AM, Chauvin D, Woo J, et al.CoV proteins at 1 μg/ml. Recombinant proteins used include SARS-CoV-2 S2 (40590-V08B), SARS-CoV-2 S1 (40591-V08H3), SARS-CoV-2 S1+S2 (40589-V08B1), MERS-CoV S2 (40070-V08), OC43 S2 (40607-V08B1), HKU1 S2 (40021-V08B)

(9) Methylene Blue Is a Nonspecific Protein–Protein Interaction Inhibitor with Potential for Repurposing as an Antiviral for COVID-19Pharmaceuticals (Basel)May 18, 2022Sung-Ting Chuang, Henrietta Papp, Anett Kuczmog, Rebecca Eells, Jose M. Condor Capcha, Lina A. Shehadeh, Ferenc Jakab, Peter BuchwaldACE2-Fc (10108-H05H), SARS-CoV-2 S1 (His tag, 40591-V08H), RBD (His tag, 40592-V08H), D614G S1 (His tag, 40591-V08H3), delta S1 (His tag, 40591-V08H23), delta RBD (His tag, 40592-V08H90), and HCoV-NL63 S1 (His tag, 40600-V08H).

(10) A cell-based ELISA as surrogate of virus neutralization assay for RBD SARS-CoV-2 specific antibodiesVaccineMarch 18, 2022Pi-Estopiñan F, Pérez MT, Fraga A, Bergado G, Díaz GD, Orosa I, Díaz M, Solozábal JA, Rodríguez LM, Garcia-Rivera D, et al.Fc-RBD (RBD-hFc) or mouse Fc-RBD (RBD-mFc) fusion proteins, or SARS-CoV-2 Spike S1 (Sino-Biological-Inc #40591-V08H3) at different concentrations were added and incubated for 2 h at RT. RBD-mFc binding was detected with

(11) ACE2 and TMPRSS2 in human saliva can adsorb to the oral mucosal epitheliumJournal of anatomyFebruary 1, 2022Zhu F, Zhong Y, Ji H, Ge R, Guo L, Song H, Wu H, Jiao P, Li S, Wang C, et al.(P0012, Beyotime, China). The SARS‐CoV‐2 spike protein (SARS‐CoV‐2 Spike S1‐His Recombinant Protein, 40591‐V08H3, Sino Biological, China) was diluted in PBS, and 1 ng/well was added into 96‐well plates (Corning, USA)

(12) Common cardiac medications potently inhibit ACE2 binding to the SARS-CoV-2 Spike, and block virus penetration and infectivity in human lung cellsScientific reportsNovember 12, 2021Caohuy H, Eidelman O, Chen T, Liu S, Yang Q, Bera A, Walton NI, Wang TT, Pollard HB.40592-VO8H), (Original) Spike [D614] S1 protein (Cat # 40591-V02H), (Original) [D614G] S1 protein (Cat # 40591-V08H3), Mink [Y453F] RBD protein (Cat # 40592-V08H80), (α/β/γ) [N501Y] RBD (Cat #40592-V08H82) and (β) (E484K)

(13) A Strategy to Detect Emerging Non-Delta SARS-CoV-2 Variants with a Monoclonal Antibody Specific for the N501 Spike ResidueDiagnostics (Basel, Switzerland)November 12, 2021Puligedda RD, Al-Saleem FH, Wirblich C, Kattala CD, Jović M, Geiszler L, Devabhaktuni H, Feuerstein GZ, Schnell MJ, Sack M, et al.

(14) An antigen microarray protocol for COVID-19 serological analysisSTAR protocolsSeptember 17, 2021Longworth J, Dittmar G."SARS S protein RBD, His Tag (MALS verified)",ACROBiosystems,Cat#SPD-S52H6 "SARS-CoV-2 Spike S1(D614G)-His Recombinant Protein, HPLC-verified",Sino Biological,Cat#40591-V08H3 "Human coronavirus HKU1 (isolate N1) (HCoV-HKU1) Spike/S1 Protein (S1 Subunit, His Tag)",Sino Biological,Cat#40021-V08H "Human coronavirus (HCoV-229E) Spike/S1 Protein (S1 Subunit, His Tag

(15) Molecular Masks for ACE2 to Effectively and Safely Block SARS-CoV-2 Virus EntryInternational journal of molecular sciencesAugust 20, 2021Shukla SP, Cho KB, Rustagi V, Gao X, Fu X, Zhang SX, Guo B, Udugamasooriya DG.amount of 0.1 µg of His-tagged recombinant SARS-CoV-2 S1 (D614G) protein (Sino Biological US Inc., Cat# 40591-V08H3, Wayne, PA, USA) was added to the column and incubated at 4 °C for 1 h. While incubating the spike protein

(16) Full-Length Recombinant hSP-D Binds and Inhibits SARS-CoV-2BiomoleculesJuly 28, 2021Arroyo R, Grant SN, Colombo M, Salvioni L, Corsi F, Truffi M, Ottolina D, Hurst B, Salzberg M, Prosperi D, et al.expressed in HEK293 cells and purchased from SinoBiologicals (#40591-V08H, #40591-V05H1, #10108-H05H, #40591-V08H3, #40591-V08H10), AcroBiosystems (#S1N-C52H3, #S1N-C52Hk, #S1N-C52Hg, #S1N-C52Hn, #S1N-C52Hp, #S1N-C52Hm),

(17) Early antibody responses associated with survival in COVID19 patientsPLoS pathogensJuly 1, 2021Zhou ZH, Dharmarajan S, Lehtimaki M, Kirshner SL, Kozlowski S.from (Raybiotech, #230–30162) and (Sino Biological #40150-V08B2); S1 ECD-His (40591-V08H), S1D614G (40591-V08H3), S2 ECD-His (40590-V08B), S1+S2 ECD (40589-V08B1) were purchased from Sino Biological; Recombinant SARS-CoV-2 Nucleocapsid Protein

(18) Humoral immune response to circulating SARS-CoV-2 variants elicited by inactivated and RBD-subunit vaccinesCell researchJuly 1, 2021Cao Y, Yisimayi A, Bai Y, Huang W, Li X, Zhang Z, Yuan T, An R, Wang J, Xiao T, et al.Inc., 0591-V49H), S1-D614G (SARS-CoV-2 Spike S1(D614G)-His Recombinant Protein, Sino Biological Inc., 40591-V08H3), S1-RBD.V2 (SARS-CoV-2 Spike S1(K417N, E484K, N501Y, D614G)-His Recombinant Protein, Sino Biological Inc

(19) Cross-reactive coronavirus antibodies with diverse epitope specificities and Fc effector functionsCell reports. MedicineJune 15, 2021Shiakolas AR, Kramer KJ, Wrapp D, Richardson SI, Schäfer A, Wall S, Wang N, Janowska K, Pilewski KA, Venkat R, et al.28807998,N/A SARS-CoV-2 S1,Sino Biological,Cat#40591-V08H SARS-CoV-2 S1 D614G,Sino Biological,Cat#40591-V08H3 SARS-CoV-2 S2,Sino Biological,Cat#40590-V08B SARS-CoV-2 RBD,Sino Biological,Cat#40592-VNAH SARS-CoV

(20) A SARS-CoV-2 neutralizing antibody with extensive Spike binding coverage and modified for optimal therapeutic outcomesNature communicationsMay 11, 2021Guo Y, Huang L, Zhang G, Yao Y, Zhou H, Shen S, Shen B, Li B, Li X, Zhang Q, et al.Sino Biological, Catalog #40592-V08H82), or SARS-CoV-2 spike S1 domain (D614G, Sino Biological, Catalog #40591-V08H3) were injected through flow cells for 60 or 180 s of association followed by a 150 or 800 s dissociation

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SARS-CoV-2 (2019-nCoV) Spike S1(D614G)-His Recombinant Protein, HPLC-verified

SARS-CoV-2 (2019-nCoV) Spike S1(D614G)-His Recombinant Protein, (HPLC-verified) from Sino Biological, Inc.

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Sino Biological, Inc.

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SARS-CoV-2 (2019-nCoV) Spike S1(D614G)-His Recombinant Protein, (HPLC-verified) from Sino Biological, Inc.

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