Hi-T4 DNA Ligase M2622L from New England Biolabs

Product Specs
ItemHi-T4 DNA Ligase
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM2622L
Quantity100000 units
ApplicationsDNA Manipulation
TypeEnzymes
Temperature-20 C
Brand/TrademarkNew England Biolabs Inc (NEB)

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) The CD2v protein of African swine fever virus inhibits macrophage migration and inflammatory cytokines expression by downregulating EGR1 expression through dampening ERK1/2 activityVeterinary ResearchNovember 15, 2023Min Zhang, Lilei Lv, Huaye Luo, Hongming Cai, Lingxue Yu, Yifeng Jiang, Fei Gao, Wu Tong, Liwei Li, Guoxin Li, et al.lentiviral vector pLV-EF1a-IRES-Hygro (Addgene, Cat#:85134) using a Hi-T4 DNA Ligase Kit (NEB, Cat#: M2622S). Similarly, the sequence encoding EGFP (enhanced green fluorescent protein) was amplified with primers

(2) An efficient cloning method to expand vector and restriction site compatibility of Golden Gate AssemblyCell Reports MethodsAugust 22, 2023Masato Sorida, Roberto Bonasio,Thermo Scientific,SM1331 BsaI-HFv2,NEB,R3733S BbsI-HF,NEB,R3539S AgeI-HF,NEB,R3552S BamHI-HF,NEB,R3136S EcoRI-HF,NEB,R3101L HindIII-HF,NEB,R3104S NcoI-HF,NEB,R3193S NotI-HF,NEB,R3189S SalI-HF,NEB,R3138S SpeI-HF,NEB,R3133S XbaI,NEB,R0145S XhoI,NEB,R0146S XmaI,NEB,R0180S KpnI-HF,NEB,R3142S NdeI,NEB,R0111S Smal,NEB,R0141S BbsI-HF,NEB,R3539M T4 DNA Ligase,NEB,M0202L Hi-T4 DNA Ligase,NEB,M2622S 100 mM ATP,NEB,P0756S NEBuilder HiFi DNA Assembly Master Mix,NEB,E2621 LB Broth,Fisher Scientific,BP9723 Agar,Fisher

(3) Improved USER cloning for TALE assembly and its application to base editingPLoS OneAugust 4, 2023Jizeng Zhou, Jiaowei Wang, Fangbing Chen, Zhenpeng Zhuang, Min Chen, Yang Yang, Xian Luo, Chengcheng Tang, Xiaoqing Zhou, Yue Chi, et al.assembled with T4 DNA Ligase (New England Biolabs, #M0202) or Hi-T4 DNA Ligase (New England Biolabs, #M2622). In constructing all the single-guide RNA (sgRNA), two complementary oligonucleotides were synthesized

(4) Transient regulation of RNA methylation in human hematopoietic stem cells promotes their homing and engraftmentLeukemiaFebruary 1, 2023Wang X, Cooper S, Broxmeyer HE, Kapur R.After annealing, double-strand oligos were ligated into the shRNA expression plasmid by T4 ligase (NEB, M2622L). Well-constructed vectors were sent to Genewiz (South Plainfield, NJ, USA) for sequencing. Lentivirus

(5) HRD1-mediated METTL14 degradation regulates m(6)A mRNA modification to suppress ER proteotoxic liver diseaseMolecular cellDecember 16, 2021Wei J, Harada BT, Lu D, Ma R, Gao B, Xu Y, Montauti E, Mani N, Chaudhuri SM, Gregory S, et al.M7449 Q5 HiFi DNA Polymerase,New England Biolabs,Cat# M0491 T4 DNA ligase,New England Biolabs,Cat# M2622S Complete proteinase inhibitor cocktail,Roche,Cat# 11697498001 Protein G Magnetic Beads,New England Biolabs

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Hi-T4 DNA Ligase

Hi-T4 DNA Ligase from New England Biolabs

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Hi-T4 DNA Ligase from New England Biolabs

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