ELISpot conjugate: Streptavidin-ALP 3310-10-1000 from Mabtech AB

Product Specs
ItemELISpot conjugate: Streptavidin-ALP
CompanyMabtech AB
Price Pricing Info Supplier Page View Company Product Page
Catalog Number3310-10-1000
Quantity1 ml
ApplicationsELISpot
Conjugate/TagALP
ComponentsStreptavidin-ALP

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Mabtech AB

Box 1233

Nacka Strand, SE-131 28

Sweden

Phone: +46 8 716 27 00
+46 8 716 27 01

Company Profile

Website: http://www.mabtech.com

(1) A Pvs25 mRNA vaccine induces complete and durable transmission-blocking immunity to Plasmodium vivaxNPJ VaccinesDecember 14, 2023Nawapol Kunkeaw, Wang Nguitragool, Eizo Takashima, Niwat Kangwanrangsan, Hiromi Muramatsu, Mayumi Tachibana, Tomoko Ishino, Paulo J. C. Lin, Ying K. Tam, Sathit Pichyangkul, et al.for 2 h at 37 °C. The plate was washed and incubated with streptavidin–alkaline phosphatase (Mabtech 3310-10-1000) for 1 h and developed by 5-bromo-4-chloro-3-indolyl phosphate-NBT-blue system (Mabtech 3650-10). The

(2) A H3K27M-targeted vaccine in adults with diffuse midline gliomaNature MedicineOctober 1, 2023Niklas Grassl, Isabel Poschke, Katharina Lindner, Lukas Bunse, Iris Mildenberger, Tamara Boschert, Kristine Jähne, Edward W. Green, Ingrid Hülsmeyer, Simone Jünger, et al.biotinylated anti-human IFN-γ antibodies (7-B6-1, Mabtech, 3420-6-250), streptavidin-ALP (Mabtech, 3310-10-1000) and ALP color development buffer (Bio-Rad, 170-6432) were used for detection of IFN-γ-producing cells.

(3) B-cell ELISpot assay to analyze human memory B cell and plasmablast responses specific to SARS-CoV-2 receptor-binding domainSTAR ProtocolsMarch 1, 2023Angeline Rouers, Matthew Zirui Tay, Lisa F.P. Ng, Laurent Renia1 in 25),Mabtech AB,Cat# 3654-TP-10 Streptavidin-ALP (working dilution: 1 in 1000),Mabtech AB,Cat#3310-10-1000 Rely+On™ Virkon™ Powder,Lanxess (VWR),Cat#148-0201 ,, Critical commercial assays,Critical commercial

(4) Protocol to detect antigen-specific nasal-resident T cells in humansSTAR ProtocolsMarch 1, 2023Joey Ming Er Lim, Anthony T. Tan, Antonio Bertoletti5420-0038 "Phosphate buffered saline, pH 7.4 (PBS)",Gibco,Cat# 10010023 Streptavidin-ALP (for ELISpot),Mabtech,Cat# 3310-10-1000 Zombie NIR Fixable Viability Kit,Biolegend,Cat# 423105 ,, Critical commercial assays,Critical commercial

(5) Long non-coding RNA-derived peptides are immunogenic and drive a potent anti-tumour responseNature CommunicationsFebruary 25, 2023Wojciech Barczak, Simon M. Carr, Geng Liu, Shonagh Munro, Annalisa Nicastri, Lian Ni Lee, Claire Hutchings, Nicola Ternette, Paul Klenerman, Alexander Kanapin, et al.plates were then washed four times with PBS then 50 µl of streptavidin-alkaline phosphatase (Mabtech, 3310-10-1000) diluted 1:750 in AD was added and incubated for 2 h at room temperature. Plates were washed four times

(6) A comparative characterization of SARS-CoV-2-specific T cells induced by mRNA or inactive virus COVID-19 vaccinesCell Reports MedicineNovember 15, 2022Joey Ming Er Lim, Shou Kit Hang, Smrithi Hariharaputran, Adeline Chia, Nicole Tan, Eng Sing Lee, Edwin Chng, Poh Lian Lim, Barnaby E. Young, David Chien Lye, et al.plus,Cytivia,Cat# 17144003 Zombie NIR Fixable Viability Kit,Biolegend,Cat# 423105 Streptavidin-ALP,Mabtech,Cat# 3310-10-1000 KPL BCIP/NBT Phosphatase substrate,SeraCare,Cat# 5420–0038 "15-mer SARS-CoV-2 overlapping Spike, Nucleoprotein

(7) Multiepitope supramolecular peptide nanofibers eliciting coordinated humoral and cellular antitumor immune responsesScience advancesJuly 22, 2022Wu Y, Wen H, Bernstein ZJ, Hainline KM, Blakney TS, Congdon KL, Snyder DJ, Sampson JH, Sanchez-Perez L, Collier JH.in a humidified CO2 incubator. IFNγ ELISPOT pairs (551881, BD), streptavidin–alkaline phosphatase (3310-10-1000, Mabtech), and Sigmafast BCIP/NBT substrate (B5655, Sigma) were used to detect and develop antigen-specific

(8) Immune boosting by B.1.1.529 ( Omicron) depends on previous SARS-CoV-2 exposureScienceJuly 15, 2022Catherine J. Reynolds et al.Plates were washed 5 times in PBST and incubated with 100 μl/well 1:1000 Streptavidin-ALP (Mabtech 3310-10-1000), in PBS/0.5% FCS for 1h, room temperature. Plates were then washed 5 times with PBST and spots developed

(9) A phenotypic signature that identifies neoantigen-reactive T cells in fresh human lung cancersCancer CellMay 1, 2022Ken-ichi Hanada, Chihao Zhao, Raul Gil-Hoyos, Jared J. Gartner, Christopher Chow-Parmer, Frank J. Lowery, Sri Krishna, Todd D. Prickett, Scott Kivitz, Maria R. Parkhurst, et al.,Sigma Aldrich,Cat# P4170 CryoStor CS10,BioLife Solutions,Cat# 210102 Streptavidin-ALP for ELISPOT,Mabtech,Cat# 3310-10-1000 Critical commercial assays,, human IFNγ DuoSet ELISA,R&D Systems,Cat#DY285B Chromium Next GEM Single

(10) Differential immunogenicity of homologous versus heterologous boost in Ad26.COV2.S vaccine recipientsMed (New York, N.Y.)February 11, 2022Khoo NKH, Lim JME, Gill US, de Alwis R, Tan N, Toh JZN, Abbott JE, Usai C, Ooi EE, Low JGH, et al.BT10549 LIVE/DEAD Fixable Blue Dead Cell Stain Kit,ThermoFisher,Cat# L23105 Streptavidin-ALP,Mabtech,Cat# 3310-10-1000 KPL BCIP/NBT Phosphatase substrate,SeraCare,Cat# 5420-0038 "15-mer SARS-COV2 overlapping Spike, Nucleoprotein

(11) Early T cell and binding antibody responses are associated with COVID-19 RNA vaccine efficacy onsetMed (New York, N.Y.)June 11, 2021Kalimuddin S, Tham CYL, Qui M, de Alwis R, Sim JXY, Lim JME, Tan HC, Syenina A, Zhang SL, Le Bert N, et al.recombinant proteins","Chemicals, peptides, and recombinant proteins" ,, Streptavidin-ALP,Mabtech,Cat# 3310-10-1000 KPL BCIP/NBT Phosphotase substrate,SeraCare,Cat# 5420-0038 Recombinant human IL-2,R7D systems,Cat#

(12) SARS-CoV-2 protein subunit vaccination of mice and rhesus macaques elicits potent and durable neutralizing antibody responsesCell reports. MedicineApril 20, 2021Mandolesi M, Sheward DJ, Hanke L, Ma J, Pushparaj P, Perez Vidakovics L, Kim C, Àdori M, Lenart K, Loré K, et al.310-02 Recombinant human IL-21,PeproTech,Cat# 200-21 Streptavidin-alkaline phosphatase,Mabtech AB,Cat# 3310-10-1000 Staphylococcal enterotoxin B,SigmaAldrich,Cat# S4881 BCIP/NBT-plus substrate,Mabtech AB,Cat# 3650-10 Brefeldin

(13) Early induction of functional SARS-CoV-2-specific T cells associates with rapid viral clearance and mild disease in COVID-19 patientsCell reportsFebruary 9, 2021Tan AT, Linster M, Tan CW, Le Bert N, Chia WN, Kunasegaran K, Zhuang Y, Tham CYL, Chia A, Smith GJD, et al.recombinant proteins","Chemicals, peptides, and recombinant proteins" ,, Streptavidin-ALP,Mabtech,Cat# 3310-10-1000 KPL BCIP/NBT phosphotase substrate,SeraCare,Cat# 5420-0038 Recombinant human IL-2,R&D Systems,Cat#

(14) Route of Vaccine Administration Alters Antigen Trafficking but Not Innate or Adaptive ImmunityCell reportsMarch 24, 2020Ols S, Yang L, Thompson EA, Pushparaj P, Tran K, Liang F, Lin A, Eriksson B, Karlsson Hedestam GB, Wyatt RT, et al.594,Invitrogen,Cat#S11227 Streptavidin-conjugated BV421,BioLegend,Cat#405225 Streptavidin-ALP,Mabtech,Cat#3310-10-1000 "DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate)",Invitrogen,"Cat# D3571, RRID:AB_2307445" ProLong Diamond Antifade Mountant

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ELISpot conjugate: Streptavidin-ALP from Mabtech AB

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ELISpot conjugate: Streptavidin-ALP from Mabtech AB

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