Thermolabile Exonuclease I M0568S from New England Biolabs

Product Specs
ItemThermolabile Exonuclease I
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0568S
Quantity3000 units
ApplicationsDNA Manipulation
TypeEnzymes
Brand/TrademarkNew England Biolabs Inc (NEB)
Temperature-20 C

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) High throughput PRIME editing screens identify functional DNA variants in the human genomebioRxivJuly 18, 2023Xingjie Ren, Han Yang, Jovia L. Nierenberg, Yifan Sun, Jiawen Chen, Cooper Beaman, Thu Pham, Mai Nobuhara, Maya Asami Takagi, Vivek Narayan, et al.previously established guidelines55. Purified PCR products were then treated with Exonuclease I (NEB, M0568L) and purified using 1× AMPure XP beads (Beckman Coulter, A63881). The isolated ePCR products were then

(2) Dissecting the immune suppressive human prostate tumor microenvironment via integrated single-cell and spatial transcriptomic analysesNature communicationsFebruary 7, 2023Hirz T, Mei S, Sarkar H, Kfoury Y, Wu S, Verhoeven BM, Subtelny AO, Zlatev DV, Wszolek MW, Salari K, et al.times then resuspended in 200 μl of ExoI mix (170 μl water, 20 μl ExoI buffer, and 10 μl ExoI (NEB, M0568)) and incubated at 37 °C for 50 min. After ExoI treatment, the beads were centrifuged for 3 min at 3000

(3) A cis-regulatory lexicon of DNA motif combinations mediating cell-type-specific gene regulationCell GenomicsNovember 9, 2022Laura K.H. Donohue, Margaret G. Guo, Yang Zhao, Namyoung Jung, Rose T. Bussat, Daniel S. Kim, Poornima H. Neela, Laura N. Kellman, Omar S. Garcia, Robin M. Meyers, et al.T4 DNA ligase,NEB,Cat#M0202L SuperScript™ IV Reverse Transcriptase,Thermo Fisher,Cat#18090200 Thermolabile Exonuclease I,NEB,Cat#M0568L Lenti-X Concentrator,Takara,Cat#631231 Turbo DNase,Thermo Fisher,Cat#AM2239 Optimem,Thermo Fisher,Cat#31985062 Lipofectamine 3000

(4) Polony gels enable amplifiable DNA stamping and spatial transcriptomics of chronic painCellNovember 8, 2022Xiaonan Fu, Li Sun, Runze Dong, Jane Y. Chen, Runglawan Silakit, Logan F. Condon, Yiing Lin, Shin Lin, Richard D. Palmiter, Liangcai GuII Q5 master mix,New England Biolabs,Cat#M0544L Thermolabile exonuclease I,New England Biolabs,Cat#M0568L Q5 HotStart polymerase,New England Biolabs,Cat#M0493L ,, Critical commercial assays,Critical commercial

(5) Comprehensive analysis and accurate quantification of unintended large gene modifications induced by CRISPR-Cas9 gene editingScience AdvancesOctober 21, 2022So Hyun Park, Mingming Cao, Yidan Pan, Timothy H. Davis, Lavanya Saxena, Harshavardhan Deshmukh, Yilei Fu, Todd Treangen, Vivien A. Sheehan, Gang Bao60°C), and extension (6 min at 65°C). After completion of PCR1, 5 μl of thermolabile exonuclease I (NEB, M0568) was added to the PCR1 reaction and incubated at 37°C for 4 min followed by heat inactivation at 80°C

(6) Regulation of Nodal signaling propagation by receptor interactions and positive feedbackeLifeSeptember 23, 2022Hannes PreiÃŸ, Anna C KÃ¶gler, David MÃ¶rsdorf, Daniel ÄŒapek, Gary H Soh, Katherine W Rogers, HernÃ¡n Morales-Navarrete, MarÃa Almuedo-Castillo, Patrick MÃ¼llerprimers: PCR amplicons were prepared for direct use in sequencing reactions by treatment with ExoI (NEB M0568) and rSAP (NEB M0371L), and the respective amplification primers were used in separate sequencing reactions.

(7) Low protein expression enhances phenotypic evolvability by intensifying selection on folding stabilityNature Ecology & EvolutionJuly 7, 2022Shraddha Karve, Pouria Dasmeh, Jia Zheng, Andreas Wagnerand 72°C/30s; 72°C/5 min. We treated the PCR product with a mix of 0.25 μl of DpnI, 0.25 μl ExoI (NEB#M0568), and 3 μl of cutsmart buffer at 37°C for 1 hour, and then deactivated the enzymes at 85°C for 20 min.

(8) Comparative molecular genomic analyses of a spontaneous rhesus macaque model of mismatch repair-deficient colorectal cancerPLOS GeneticsApril 21, 2022Nejla Ozirmak Lermi et al.products was performed with an in-house ExoSAP solution [50 μL of Exonuclease I (20,000 units/mL, NEB M0568, Ipswich, MA); 40 μL of Antarctic Phosphatase (5,000 units/ml); 16 μL of Antarctic Phosphatase buffer

(9) Locus-specific expression of transposable elements in single cells with CELLO-seqNature BiotechnologyApril 1, 2022Rebecca V. Berrens, Andrian Yang, Christopher E. Laumer, Aaron T. L. Lun, Florian Bieberich, Cheuk-Ting Law, Guocheng Lan, Maria Imaz, Joseph S. Bowness, Neil Brockdorff, et al.(DNase/RNase-Free Distilled H2O, Thermo Fisher Scientific #10977-049), 0.5 μl of Exonuclease I (NEB #M0568) in 0.5 μl of 10x Cutsmart buffer (NEB # B7204S). We incubated the reaction at 37 °C for 1h, followed

(10) Mapping and CRISPR homology-directed repair of a recessive white eye mutation in Plodia mothsiScienceMarch 18, 2022Christa Heryanto, Joseph J. Hanly, Anyi Mazo-Vargas, Amruta Tendolkar, Arnaud MartinFisher Scientific,F-170S NlaIV Restriction Enzyme,New England Biolabs,R0126S ExoI,New England Biolabs,M0568 rSAP,New England Biolabs,M0371S Cas9-NLS protein,"QB3 Macrolab, UC Berkeley", RNAse Cocktail Enzyme

(11) Assembly of Longâ€Adapter Singleâ€Strand Oligonucleotide (LASSO) Probes for Massively Parallel Capture of Kilobase Size DNA TargetsCurrent ProtocolsDecember 2, 2021Lamia Chkaiban, Lorenzo Tosi, Biju ParekkadanLambda Exonuclease (New England BioLabs, cat. no. M0262S) Exonuclease I (New England BioLabs, cat. no. M0568S) Exonuclease III (New England BioLabs, cat. no. M0206S) NEBNext Ultra DNA Library Prep Kit (New

(12) De novo deletions and duplications at recombination hotspots in mouse germlinesCellNovember 12, 2021Agnieszka Lukaszewicz, Julian Lange, Scott Keeney, Maria JasinBiolabs,N/A Exo V (RecBCD),New England Biolabs,Cat# M0345S Thermolabile ExoI,New England Biolabs,Cat# M0568S "Proteinase K, PCR-grade",Roche,Cat# 03115828001 ,, Critical commercial assays,, ,, Invitrogen

(13) Optical Biosensor Platforms Display Varying Sensitivity for the Direct Detection of Influenza RNABiosensors (Basel)September 30, 2021Samantha J. Courtney et al.at least 3 donors (Lee Biosolutions, cat. no. 991-05-P). Then, 20 U Thermolabile Exonuclease I (NEB M0568L) was added and volume brought to 20 µL with 1X NEB 3.1 buffer and incubated at 37 °C for 30 min. After

(14) The RNA-binding protein Puf5 contributes to buffering of mRNA upon chromatin-mediated changes in nascent transcriptionJournal of cell scienceAugust 1, 2021Kochan DZ, Mawer JSP, Massen J, Tishinov K, Parekh S, Graef M, Spang A, Tessarz P.1:1 (v/v). Purified DNA was eluted in 17 μl H2O and treated with exonuclease (New England Biolabs, #M0568). Subsequently, cDNA was amplified and the final PCR product was purified with Ampure XP beads at a

(15) Highly sensitive spatial transcriptomics at near-cellular resolution with Slide-seqV2Nature BiotechnologyMarch 1, 2021Robert R. Stickels, Evan Murray, Pawan Kumar, Jilong Li, Jamie L. Marshall, Daniela J. Di Bella, Paola Arlotta, Evan Z. Macosko, Fei ChenTris pH 8.0 1 mM EDTA 0.01% Tween-20 ExoI mix: 170μl H20 20μl ExoI buffer 10μl ExoI (NEB M0568) After ExoI treatment the beads were centrifuged for 3 minutes at 3000 RCF. The supernatant was then

Be the First to Write a Review!

Thermolabile Exonuclease I

Thermolabile Exonuclease I from New England Biolabs

Description

New England Biolabs

Citations (15)

Reviews

Thermolabile Exonuclease I from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map