PNGase F (Glycerol-free), Recombinant P0709S from New England Biolabs

Product Specs
ItemPNGase F (Glycerol-free), Recombinant
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberP0709S
Quantity15000 units
ApplicationsProteomics & Glycomics
Brand/TrademarkNew England Biolabs Inc (NEB)
Temperature4 C
TypeGlycosidases

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Establishment of a CPER reverse genetics system for Powassan virus defines attenuating NS1 glycosylation sites and an infectious NS1-GFP11 reporter virusmBioJuly 25, 2023Jonas N. Conde, Grace E. Himmler, Megan C. Mladinich, Yin Xiang Setoh, Alberto A. Amarilla, William R. Schutt, Nicholas Saladino, Elena E. Gorbunova, Daniel J. Salamango, Jorge Benach, et al.above, and 20 µg of lysate or 50 µL of supernatants was subjected to Endo H (P0702S NEB) or PNGase F (P0709S NEB) digestion following the manufacturer’s protocols. Samples were resolved by SDS-10% polyacrylamide gel electrophoresis

(2) A role for endothelial alpha-mannosidase MAN1C1 in radiation-induced immune cell recruitmentiScienceDecember 22, 2022Ladaigue S, Lefranc AC, Balde K, Quitoco M, Bacquer E, Busso D, Piton G, Dépagne J, Déchamps N, Yamakawa N, et al.bioultra,Sigma-Aldrich,Cat#: I1149-5G Trichloroacetic acid,Sigma-Aldrich,Cat#: T9159-500G PNGase F,New England Biolabs,Cat#: P0709S Acetone,Carlo Erba,Cat#: 412501 Trypsin,Sigma-Aldrich,Cat#: T1005 Acetonitrile,Sigma-Aldrich,Cat#:

(3) SARS-CoV-2 spike protein variant binding affinity to an angiotensin-converting enzyme 2 fusion glycoproteinsPLOS ONEDecember 6, 2022Alicia M. Matthews, Thomas G. Biel, Uriel Ortega-Rodriguez, Vincent M. Falkowski, Xin Bush, Talia Faison, Hang Xie, Cyrus Agarabi, V. Ashutosh Rao, Tongzhong Jubicarbonate (pH 7.5). Finally, 50 mM ammonium bicarbonate containing 500U of glycerol-free PNGase F (Cat# P0709S, NEB) was added and the ultra-filtration cell was transferred to a fresh collection tube and incubated

(4) Deciphering O-glycoprotease substrate preferences with O-Pair Search†Molecular OmicsDecember 5, 2022Nicholas M. Riley, Carolyn R. Bertozzico-incubated with the O-glycoprotease. Following O-glycoprotease digestion, 1 μl PNGaseF (New England Biolabs, P0709S, diluted to 10 000 U ml−1 in PBS) was added to each digestion for an overnight (~12 hour) incubation

(5) Mucus sialylation determines intestinal host-commensal homeostasisCellMarch 17, 2022Yikun Yao, Girak Kim, Samantha Shafer, Zuojia Chen, Satoshi Kubo, Yanlong Ji, Jialie Luo, Weiming Yang, Sebastian P. Perner, Chrysi Kanellopoulou, et al.removing total glycosylation, NEB, cat: P6044S), PNGase F (for removing N-linked glycosylation, NEB, cat: P0709S), O-Glycosidase (NEB, P0733S) plus a2–3,6,8,9 Neuraminidase A (NEB, cat: P0722S) (for removing O-linked

(6) ALFY localizes to early endosomes and cellular protrusions to facilitate directional cell migrationJournal of cell scienceFebruary 15, 2022Søreng K, Pankiv S, Bergsmark C, Haugsten EM, Dahl AK, de la Ballina LR, Yamamoto A, Lystad AH, Simonsen A.ice, supplemented with 2 µl of 10× GlycoBuffer2 (NEB, B3704S), 2 µl of 10% NP40, 1 µl of PNGaseF (NEB, P0709S) and 5 µl of water and incubated for 1 h at 37°C. For treatment with CIP, 9 µl of cell lysate was supplemented

(7) Molecular architecture and modifications of full-length myocilinExperimental Eye ResearchOctober 1, 2021Mackenzie D. Martin, Dustin J. E. Huard, Ricardo C. Guerrero-Ferreira, Ishani M. Desai, Brett M. Barlow, Raquel L. Liebermanenzyme kit (New England Biolabs (NEB) P6044S) and individual deglycosylation enzymes from the kit (NEB P0709S, P0733S, P0722S, P0745S, and P0721S or Promega V4831) according to associated NEB and Promega protocols.

(8) Glycoproteomics Sample Processing, LC-MS, and Data Analysis Using GlycReSoftCurrent ProtocolsJuly 23, 2021Meizhe Wang, Asif Shajahan, Lauren E. Pepi, Parastoo Azadi, Joseph Zaiawater) Sequencing-grade modified trypsin (Cat. No. V5113, Promega) 1 M sodium phosphate buffer, pH 6.8 PNGase F (Cat. No. P0709S, New England Biolabs) 5 % acetic acid (acetic acid, Cat. No. 64–19-7, Sigma Aldrich, water, Cat. No.

(9) Molecular Stressors Engender Protein Connectivity Dysfunction through Aberrant N-Glycosylation of a ChaperoneCell reportsJune 30, 2020Yan P, Patel HJ, Sharma S, Corben A, Wang T, Panchal P, Yang C, Sun W, Araujo TL, Rodina A, et al.Clinical Pharmacy. Lambda protein Phosphatase (Lambda PPase, P0753S), Endo H (P0703S) and PNGase F (P0709S) were purchased from NEB Inc. High capacity Streptavidin Agarose (20361) was purchased from ThermoFisher Scientific

(10) Type 2 diabetes-associated single nucleotide polymorphism in Sorcs1 gene results in alternative processing of the Sorcs1 protein in INS1 β-cellsScientific ReportsDecember 19, 2019Belinda Yau, Zachary Blood, Yousun An, Zhiduan Su, Melkam A. Kebedeby western blot, WCL were treated with either 1250 U or 2500 U Endo H (NEB, P0702S) or 500 U PNGase F (NEB, P0709S) under denaturing conditions as per manufacturer’s protocols, after overnight induction of Sorcs1 expression

(11) Endothelial-to-Mesenchymal Transition in Human Adipose Tissue Vasculature Alters the Particulate Secretome and Induces Endothelial DysfunctionArteriosclerosis, thrombosis, and vascular biologyOctober 1, 2019Haynes BA, Yang LF, Huyck RW, Lehrer EJ, Turner JM, Barabutis N, Correll VL, Mathiesen A, McPheat W, Semmes OJ, et al.digestion, peptides were subjected to deglycosylation with 1000 Unit PNGase F (New England Biolabs, cat#: P0709S) for 6 hrs at 37°C. Digested peptides were desalted and purified using C18 pipette tips (Thermo Fisher Scientific

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PNGase F (Glycerol-free), Recombinant

PNGase F (Glycerol-free), Recombinant from New England Biolabs

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New England Biolabs

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PNGase F (Glycerol-free), Recombinant from New England Biolabs

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