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Interleukin-6 human
Interleukin-6 human from MilliporeSigma
Be the first to write a review!
Citations:
(21)
Supplier Page
Supplier Page from
MilliporeSigma for
Interleukin-6 human
Description
IL-6, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Interleukin-6 human
Company
MilliporeSigma
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
I1395
Molecular Weight
26 kDa
Species
human
Molecule Name
ACE, ECE1, Ace
NCBI Full Gene Name
angiotensin I converting enzyme, endothelin converting enzyme 1
NCBI Gene Aliases
Dcp1, StsRR92, DCP1, CD143, ACE1, ECE, DCP
Applications
mammalian cell culture
Format
Lyophilized from a 0.2 µm filtered solution of phosphate buffer saline (PBS), pH 7.4, containing 500 µg bovine serum albumin
Quantity
10µG, 10µG, 50µG, 50µG
Add to Compare List
MilliporeSigma
400 Summit Drive
Burlington
,
Massachusetts
01803
United States
Phone
: (800)-645-5476
(800)-645-5439
Company Profile
Website:
www.SigmaAldrich.com/
Citations (21)
(1)
The Impact of Hydroxychloroquine on Primary Feto-Placental Endothelial Cells from Healthy and Early-Onset Preeclamptic Placentas
International Journal of Molecular Sciences
June 30, 2023
Maja Gajić, Bianca Schröder-Heurich, Monika Horvat Mercnik, Mila Cervar-Zivkovic, Christian Wadsack, Frauke von Versen-Höynck, Karoline Mayer-Pickel
Germany) to the concentration of 10 mg/mL. Cytokines: TNF-α (
H8916
,
Merck
, Darmstadt, Germany), IL-6 (
I1395
,
Merck
, Darmstadt, Germany) and
recombinant human IL-1β
(200-Q1B,
PeproTech
, Cranbury, NJ, USA) were
(2)
Airway surface hyperviscosity and defective mucociliary transport by IL-17/TNF-α are corrected by beta-adrenergic stimulus
JCI Insight
November 22, 2022
Daniela Guidone, Martina Buccirossi, Paolo Scudieri, Michele Genovese, Sergio Sarnataro, Rossella De Cegli, Federico Cresta, Vito Terlizzi, Gabrielle Planelles, Gilles Crambert,
et al.
Sigma-Aldrich
), IFN-γ (10 ng/mL,
I3265
,
Sigma-Aldrich
), IL-1β (10 ng/mL
H6291
,
Sigma-Aldrich
), or IL-6 (10 ng/mL,
I1395
,
Sigma-Aldrich
).
Immunofluorescence
of cultured epithelia. Snapwell supports carrying differentiated
(3)
Isolation of human and mouse myeloid-derived suppressor cells for metabolic analysis
STAR protocols
June 17, 2022
Choi JE, MacDonald CR, Gandhi N, Das G, Repasky EA, Mohammadpour H.
Red,MilliporeSigma,Catalog # P3532 Mouse IL-6,
MilliporeSigma
,Catalog #
I9646
Human IL-6,
MilliporeSigma
,Catalog #
I1395
Mouse GM-CSF,
BioLegend
,Catalog #
713704
Human GM-CSF,
BioLegend
,Catalog #
713604
Detachin Cell Detachment
(4)
Akt-Dependent Glycolysis-Driven Lipogenesis Supports Proliferation and Survival of Human Pulmonary Arterial Smooth Muscle Cells in Pulmonary Hypertension
Frontiers in medicine
January 1, 2022
Jiang L, Goncharov DA, Shen Y, Lin D, Chang B, Pena A, DeLisser H, Goncharova EA, Kudryashova TV.
Cell Signaling
(Danvers, MA, United States).
Inhibitors
and Activators 2-Deoxy-D-glucose (2-DG, D6134), IL-6 (
I1395
), PDGF-BB (
GF149
), and Akt inhibitor VIII (SIAL-
124018
) were purchased from
Millipore Sigma
(St. Louis,
(5)
Dihydromyricetin ameliorates osteogenic differentiation of human aortic valve interstitial cells by targeting c-KIT/interleukin-6 signaling pathway
Frontiers in pharmacology
January 1, 2022
Zhang S, Fan L, Wang Y, Xu J, Shen Q, Xie J, Zeng Z, Zhou T.
final concentration in OM) was used to induce phosphorylation of c-KIT.
IL-6
(
Sigma–Aldrich
, Catalog#
I1395
; at 50 ng/ml final concentration in OM) was used to explore the underlying mechanism of the DHM-mediated
(6)
Analytical and clinical evaluation of a novel assay for measurement of interleukin 6 in human whole blood samples
Journal of Clinical Laboratory Analysis
November 1, 2021
Zhicheng Ye, Niuniu Dong, Keqing Liang, Haixia Hu, Jin Xu
limit of detection
. A high‐concentration pool was prepared by spiking recombinant IL‐6 (
Sigma Aldrich
, product no.
I1395
) into an aliquot of the pooled sample to a final concentration of ~45,000 pg/ml. Six different analyte
(7)
β2-adrenergic receptor signaling regulates metabolic pathways critical to myeloid-derived suppressor cell function within the TME
Cell reports
October 26, 2021
Mohammadpour H, MacDonald CR, McCarthy PL, Abrams SI, Repasky EA.
C57/BL6
mice
in 100 μLs of PBS. METHOD DETAILS Reagents used
Mouse
(Catalog
I9646
) and
human
(Catalog
I1395
) IL-6 were from
MilliporeSigma
.
Mouse
(Catalog
713704
) and
human
(Catalog
713604
)
GM-CSF
and
mouse
(Catalog
(8)
Silencing of
ISLR
inhibits tumour progression and glycolysis by inactivating the IL‑6/JAK/STAT3 pathway in non‑small cell lung cancer
International Journal of Molecular Medicine
October 26, 2021
Peng Zhang, Zhen Li, Guangming Yang
constant-temperature incubator (Thermo Fisher Scientific, Inc.) with 5% CO2 at 37°C. Human recombinant IL-6 (cat. no.
I1395
-50UG;
Sigma-Aldrich
; Merck KGaA) was dissolved in PBS to a concentration of 100 µg/ml as a stock solution
(9)
Stromal induction of BRD4 phosphorylation Results in Chromatin Remodeling and BET inhibitor Resistance in Colorectal Cancer
Nature communications
July 21, 2021
Wang W, Tang YA, Xiao Q, Lee WC, Cheng B, Niu Z, Oguz G, Feng M, Lee PL, Li B,
et al
.
following recombinant proteins were used in cell treatments: Recombinant human IL-6 (
Sigma-Aldrich
, Cat#
I1395
), Recombinant human IL-8 (
PeproTech
, Cat#
200-08
), human TNFα (PeproTech, Cat#300-01 A),
human
IL-1β
(10)
Tumor exposed-lymphatic endothelial cells promote primary tumor growth via IL6
Cancer Letters
January 28, 2021
Maureen Van de Velde, Marie Ebroin, Tania Durré, Marc Joiret, Lionel Gillot, Silvia Blacher, Liesbet Geris, Frédéric Kridelka, Agnès Noel
(
15140
-122) (all from
Thermofisher
, MA). Serum-starved
tumor
cells were treated with recombinant human IL6 (
I1395
,
Sigma
, Belgium) and a neutralizing antibody against
IL6
(2.5 μg/ml, MAB 2061, R&D Systems, MN). LEC
(11)
Iron overload inhibits BMP/SMAD and IL-6/STAT3 signaling to hepcidin in cultured hepatocytes
PloS one
January 1, 2021
Charlebois E, Pantopoulos K.
from
Sigma-Aldrich
. Following recombinant proteins were used: murine IL-6 (#5216, Cell Signaling), human IL-6 (#
I1395
,
Sigma-Aldrich
),
human
BMP6 (#507-BP, R&D Systems), and
human
holo-transferrin (#T4132, Sigma). Salicylaldehyde
(12)
YAP and TAZ protect against white adipocyte cell death during obesity
Nature communications
October 28, 2020
Wang L, Wang S, Shi Y, Li R, Günther S, Ong YT, Potente M, Yuan Z, Liu E, Offermanns S.
wortmannin (catalog number
W3144
), latrunculin B (catalog number 428020), and IL-6 (catalog number
I1395
) were from
Sigma-Aldrich
.
SC514
was from
Santa Cruz Biotechnology
(catalog number 354812-17-2).
SP600125
(13)
Delineating Astrocytic Cytokine Responses in a Human Stem Cell Model of Neural Trauma
Journal of Neurotrauma
January 1, 2020
Eric Peter Thelin et al.
control samples (technical repeats).
Human
recombinant IL-1β (#11457756001), IL-4 (#
I4269
), IL-6 (#
I1395
), IL-10 (#
IL010
), and TNF (#
T6674
) (all from
Merck
, Kenilworth, NJ) were each sourced as lyophilized
(14)
Impact of Proinflammatory Cytokines on the Virulence of Uropathogenic Escherichia coli
Frontiers in Microbiology
May 9, 2019
Ulrik Engelsöy, Ignacio Rangel, Isak Demirel
CaCl2, and 0.25% Casamino Acids) with or without the presence of TNF-α (
T6674
), IL-1β (
I9401
), IL-6 (
I1395
), IL-8 (
I1645
) or IFN-γ (
SRP3058
) (0.5, 1, 10, 20 or 40 ng/ml, all from
Sigma-Aldrich
, St. Louis, MO,
(15)
Hindlimb Ischemia Impairs Endothelial Recovery and Increases Neointimal Proliferation in the Carotid Artery
Scientific reports
January 15, 2018
Sorrentino S, Iaconetti C, De Rosa S, Polimeni A, Sabatino J, Gareri C, Passafaro F, Mancuso T, Tammè L, Mignogna C,
et al
.
investigate the effect of pro-inflammatory cytokine on miR-16 expression, HUVECs were incubated with IL-6 (
I1395
–10UG;
Sigma Aldrich
), IL-8 (
I1645
–10UG;
Sigma Aldrich
), IL-1β (
I9401
–5UG;
Sigma Aldrich
), and TNF-α
(16)
Norcantharidin inhibits IL-6-induced epithelial-mesenchymal transition via the JAK2/STAT3/TWIST signaling pathway in hepatocellular carcinoma cells
Oncology Reports
February 1, 2017
Yebo Gao, Weidong Li, Rui Liu, Qiujun Guo, Jie Li, Yanju Bao, Honggang Zheng, Shulong Jiang, Baojin Hua
diluted to a concentration of 0.5 µM in culture medium before use (DMSO <1%). Human recombinant IL-6 (#
I1395
-50UG;
Sigma-Aldrich
) was dissolved in phosphate-buffered saline (PBS) to a concentration of 100 µg/ml
(17)
Exposure to concentrated ambient fine particulate matter disrupts vascular endothelial cell barrier function via the IL-6/HIF-1α signaling pathway
FEBS open bio
July 1, 2016
Dai J, Sun C, Yao Z, Chen W, Yu L, Long M.
KeyGen Biotech (Nanjing, China). TRIzol was obtained from Invitrogen (CA, USA). Recombinant human IL‐6 (
I1395
) was obtained from
Sigma‐Aldrich
(St. Louis, MO, USA). The siRNA duplex for
HIF‐1α
was synthesized by
(18)
Insufficiency of phosphatidylethanolamine N-methyltransferase is risk for lean non-alcoholic steatohepatitis
Scientific Reports
April 1, 2016
Atsuko Nakatsuka et al.
glucose (25 mM), 100 nM insulin (
I9278
,
SIGMA
), 250 μM palmitate (P0500, SIGMA), and 20 ng/ml IL-6 (
I1395
,
SIGMA
). For the preparation of palmitate stock solution, 500 mM palmitate in DMSO was diluted to 5
(19)
Human Head and Neck Squamous Cell Carcinoma-Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor Cells
Journal of immunology (Baltimore, Md. : 1950)
February 1, 2016
Younis RH, Han KL, Webb TJ.
Recombinant human GM-CSF
(catalog no.
215-GM-010
) (
R&D Systems
Minneapolis, MN) and IL-6 recombinant human (catalog no.
I1395
) from (
Sigma-Aldrich
) were also used. Griess reagent (catalog no.
G2930
) (
Promega
, Madison, WI) was
(20)
Downregulation of duodenal SLC transporters and activation of proinflammatory signaling constitute the early response to high altitude in humans
American journal of physiology. Gastrointestinal and liver physiology
October 1, 2014
Wojtal KA, Cee A, Lang S, Götze O, Frühauf H, Geier A, Pastor-Anglada M, Torres-Torronteras J, Martí R, Fried M,
et al
.
anti-HIF-2␣ was purchased from Abcam (Cambridge, UK).
Human
recombinant IFN-␥ (IFG40012) and IL-6 (
I1395
), and CoCl2 (
15862
) were purchased from
SigmaAldrich
(Buchs, Switzerland).
FM19G11
(
sc-364490
) and FR180204 (
sc-203945
)
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Interleukin-6 human from MilliporeSigma
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
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Reviews
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