Fibronectin human plasma F1056 from MilliporeSigma

Product Specs
ItemFibronectin human plasma
CompanyMilliporeSigma
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberF1056
Specieshuman plasma
Molecule NameIl4, CDC2, GSK3A
NCBI Full Gene Namecyclin dependent kinase 1, glycogen synthase kinase 3 alpha, interleukin 4
NCBI Gene AliasesCDC28A, CDC2, P34CDC2, Il4e12
Applicationsmammalian cell culture
Quantity1MG, 2MG, 5MG

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MilliporeSigma

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Burlington, Massachusetts 01803

United States

Phone: (800)-645-5476
(800)-645-5439

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Website: www.SigmaAldrich.com/

(1) Deubiquitinase USP1 influences the dedifferentiation of mouse pancreatic β-cellsiScienceMay 19, 2023Meenal Francis, Smitha Bhaskar, Saarwani Komanduri, Preethi Sheshadri, Jyothi Prasanna, Anujith Kumarharvested for analysis. Handpicked endogenous islets were plated on 10 μg/ml fibronectin (Sigma Aldrich, F1056) coated wells and maintained in RPMI media (Gibco, 11875-093) containing 10% FBS, 2 mM Glutamax, Penicillin

(2) Anastellin impacts on the processing of extracellular matrix fibronectin and stimulates release of cytokines from coronary artery smooth muscle cellsScientific reportsDecember 21, 2022He J, Steffen JH, Thulstrup PW, Pedersen JN, Sauerland MB, Otzen DE, Hawkins CL, Gourdon P, Davies MJ, Hägglund P.importance. Experimental procedures Materials All chemicals, including lyophilized human plasma FN (F1056) and anti-FN monoclonal antibody (mAb) 3E2, were purchased from Sigma-Aldrich (St Louis, Missouri, USA),

(3) Transcriptomic Analysis of Human Fragile X Syndrome Neurons Reveals Neurite Outgrowth Modulation by the TGFβ/BMP PathwayInternational Journal of Molecular SciencesAugust 17, 2022Liron Kuznitsov-Yanovsky, Guy Shapira, Lital Gildin, Noam Shomron, Dalit Ben-YosefPBS, and plates were coated with 1 μg/mL Laminin I (L2020, Sigma-Aldrich) and 2 μg/mL Fibronectin (F1056, Sigma-Aldrich) in PBS, air dried for 45 min at room temperature, and kept at 4 °C. 4.3. Immunofluorescence Cells

(4) Intrinsic antiviral immunity of barrier cells revealed by an iPSC-derived blood-brain barrier cellular modelCell reportsMay 31, 2022Cheng Y, Medina A, Yao Z, Basu M, Natekar JP, Lang J, Sanchez E, Nkembo MB, Xu C, Qian X, et al.Penicillin",Gibco,Cat#15240062 Normal Goat Serum,ThermoFisher Scientific,Cat#10000C Fibronectin from human plasma,Sigma-Aldrich,Cat#F1056 "Collagen type I, Rat tail",Sigma-Aldrich,Cat#08-115 N2 Supplement,GIBCO,Cat#17502048 B27 Supplements,GIBCO,Cat#17504044 Recombinant

(5) NADH oxidase of Mycoplasma hyopneumoniae functions as a potential mediator of virulenceBMC veterinary researchApril 2, 2022Hao F, Xie X, Feng Z, Chen R, Wei Y, Liu J, Xiong Q, Shao G, Lin J.PBS containing 0.5% Tween 20 (PBST) for 2 h at 37 °C. Two hundred micrograms of fibronectin (Cat No. F1056, Sigma–Aldrich, St Louis, MO, USA) in 100 μL of PBST was applied to the wells, and ELISA plates were

(6) Parallelized label-free monitoring of cell adhesion on extracellular matrix proteins measured by single colour reflectometryAnalytical and Bioanalytical ChemistryJanuary 1, 2022Johanna Hutterer, GÃ¼nther Proll, Peter Fechner, GÃ¼nter Gauglitzpresenting quadruplets of 5 different ECM proteins. For the spotting of the proteins, fibronectin (F1056, Merck KGaA, Darmstadt, Germany), vitronectin (SRP3186, Merck KGaA, Darmstadt, Germany), collagen IV

(7) The molecular conformation of silk fibroin regulates osteogenic cell behavior by modulating the stability of the adsorbed protein-material interfaceBone ResearchDecember 1, 2021Yanlin Long, Xian Cheng, John A. Jansen, Sander G. C. Leeuwenburgh, Jing Mao, Fang Yang, Lili Chenat a specified time point (n = 3). Detachment resistance of FN FN (5 μg·mL−1) from human plasma (F1056, Sigma, U.S.A.) was dissolved in PBS solution according to previous references.19,22 The SF substrates

(8) High Glycolytic Activity Enhances Stem Cell Reprogramming of Fahd1-KO Mouse Embryonic FibroblastsCellsAugust 10, 2021Salti A, Etemad S, Cubero MS, Albertini E, Kovacs-Szalka B, Holzknecht M, Cappuccio E, Cavinato M, Edenhofer F, Jansen Dürr P.0.24% NaHCO3 (Gibco, 25080094), 2 mM L-Glutamine (Gibco, 25030081), 5 mg/mL fibronectin (Sigma-Aldrich, F1056), and 1x insulin/transferrin/sodium selenite supplement (Sigma-Aldrich, I9278). At this point, neural

(9) Distinct roles of nonmuscle myosin II isoforms for establishing tension and elasticity during cell morphodynamicseLifeAugust 10, 2021Weißenbruch K, Grewe J, Hippler M, Fladung M, Tremmel M, Stricker K, Schwarz US, Bastmeyer M.Micropatterned coverslips were functionalized with a solution of 10 µg ml−1 FN from human plasma (Sigma Aldrich #F1056) for 1 hr at room temperature. For direct microcontact printing, stamps were incubated for 10 min with

(10) Dynamic persistence of UPEC intracellular bacterial communities in a human bladder-chip model of urinary tract infectioneLifeJuly 5, 2021Sharma K, Dhar N, Thacker VV, Simonet TM, Signorino-Gelo F, Knott GW, McKinney JD.A10037,IF (2 μg/ml) "Peptide, recombinant protein",Fibronectin from human plasma,Sigma-Aldrich,Cat#: F1056,500 µg/ml in ddH2O "Peptide, recombinant protein","Native Collagen, Bovine dermis",AteloCell,Cat#:

(11) Cross-linking and modification of fibronectin by peroxynitrous acid: Mapping and quantification of damage provides a new model for domain interactionsJournal of Biological ChemistryFebruary 2, 2021Michele Mariotti, Adelina Rogowska-Wrzesinska, Per HÃ¤gglund, Michael J. Daviesprocedures Reagents and proteins All chemicals including lyophilized human plasma FN (catalog number: F1056) were purchased from Sigma-Aldrich unless stated otherwise. 18O water (95.7% pure) was purchased from

(12) Fibronectin adsorption on oxygen plasma-treated polyurethane surfaces modulates endothelial cell responseJournal of Materials Chemistry BJanuary 22, 2021Ruben Daum, Ivana Mrsic, Johanna Hutterer, Achim Junginger, Svenja Hinderer, Alfred J. Meixner, Günter Gauglitz, Thomas Chassé, Katja Schenke-Laylandconstant at 100 W. 2.1.2 DY-490 labeling of fibronectin. For covalent, fluorescence labeling of human FN (F1056-2MG, Sigma-Aldrich, St. Louis, USA), Dyomics DY-490 N-Hydroxysuccinimid-Ester (FluoroSpin 490 Protein Labeling

(13) Nicotinamide Adenine Dinucleotide-Dependent Flavin Oxidoreductase of Mycoplasma hyopneumoniae Functions as a Potential Novel Virulence Factor and Not Only as a Metabolic EnzymeFrontiers in microbiologyJanuary 1, 2021Xie X, Hao F, Chen R, Wang J, Wei Y, Liu J, Wang H, Zhang Z, Bai Y, Shao G, et al.incubator for 2 h with gentle shaking. Then, the membrane was incubated with 15 μg/mL fibronectin (Cat No. F1056, Sigma-Aldrich, Darmstadt, Germany) or plasminogen (Cat No. SRP6518, Sigma-Aldrich, Darmstadt, Germany)

(14) In situ molecular imaging of adsorbed protein films in water indicating hydrophobicity and hydrophilicityScientific ReportsDecember 1, 2020Jiachao Yu, Yufan Zhou, Mark Engelhard, Yuchen Zhang, Jiyoung Son, Songqin Liu, Zihua Zhu, Xiao-Ying Yuincluding BSA (product number A4161), collagen (type IV, product number C8374), fibronectin (product number F1056), laminin (product number L6274) and vitronectin (product number V8379) were studied in this work, all

(15) A lung-on-chip model of early Mycobacterium tuberculosis infection reveals an essential role for alveolar epithelial cells in controlling bacterial growtheLifeNovember 24, 2020Thacker VV, Dhar N, Sharma K, Barrile R, Karalis K, McKinney JD.,Miltenyi Biotec,Cat#: 130-095-927, "Peptide, recombinant protein",Fibronectin from human plasma,Sigma-Aldrich,Cat#: F1056, Peptide recombinant protein,"Native Collagen, Bovine dermis",AteloCell,Cat#: IAC-50,5 mg/ml "Software,

(16) Differentiation of ciliated human midbrain-derived LUHMES neuronsJournal of cell scienceNovember 9, 2020Lauter G, Coschiera A, Yoshihara M, Sugiaman-Trapman D, Ezer S, Sethurathinam S, Katayama S, Kere J, Swoboda P.flasks) pre-coated with poly-Lornithine hydrobromide (Sigma P3655; 50 µg/ml) and human fibronectin (Sigma F1056; 1 µg/ml). The growth medium was DMEM/F-12 Ham (Sigma D6421-6) supplemented with L-glutamine (Sigma

(17) Peroxynitrous acid (ONOOH) modifies the structure of anastellin and influences its capacity to polymerize fibronectinRedox BiologySeptember 1, 2020Jianfei He, Eva Ramos Becares, Peter Waaben Thulstrup, Luke F. Gamon, Jannik Nedergaard Pedersen, Daniel Otzen, Pontus Gourdon, Michael J. Davies, Per HÃ¤gglundpolymerization. 2 Materials and methods 2.1 Materials All chemicals including lyophilized human plasma FN (#F1056) and anti-3-nitroTyr polyclonal antibody (pAb; #06-284) were from Sigma-Aldrich (St Louis, Missouri,

(18) Role of the nuclear membrane protein Emerin in front-rear polarity of the nucleusNature communicationsMay 1, 2020Nastały P, Purushothaman D, Marchesi S, Poli A, Lendenmann T, Kidiyoor GR, Beznoussenko GV, Lavore S, Romano OM, Poulikakos D, et al.Then, coverslips were incubated with an extracellular matrix protein fibronectin (Sigma-Aldrich, Cat. F1056, 25 μg/ml in 100 mM NaHCO3 pH 8.4). Cells were detached using EDTA 0.02% (Versane, Gibco, Cat. E6758)

(19) Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro CultureCellsMarch 23, 2020Daum R, Visser D, Wild C, Kutuzova L, Schneider M, Lorenz G, Weiss M, Hinderer S, Stock UA, Seifert M, et al.functionalized by protein adsorption. They were incubated for 2 h at 37 °C with 20 µg/mL human plasma FN (F1056, Sigma-Aldrich, St. Louis, USA) or 20 µg/mL recombinant full-length human DCN [25], individually or

(20) Axon-seq for in Depth Analysis of the RNA Content of Neuronal ProcessesBio-protocolJuly 20, 2019Nijssen J, Aguila J, Hedlund E.(Sigma-Aldrich, catalog number: A-004-C) 5. Fibronectin, 1 mg/ml solution (Sigma-Aldrich, catalog number: F1056) 6. Laminin, 1 mg/ml solution (Sigma-Aldrich, catalog number: L2020) 7. TrypLe Express (Thermo Fisher,

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Fibronectin human plasma from MilliporeSigma

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Fibronectin human plasma from MilliporeSigma

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