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Platelet-Derived Growth Factor-BB human
Platelet-Derived Growth Factor-BB human from MilliporeSigma
Be the first to write a review!
Citations:
(19)
Supplier Page
Supplier Page from
MilliporeSigma for
Platelet-Derived Growth Factor-BB human
Description
Platelet-Derived Growth Factor-BB human has been used :
As a growth supplement in proximal tubule and glomerular explant cell culture<REFERENCE ID="1013558" TYPE="literature"/>
In three-dimensional scaffold preparation<REFERENCE ID=
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Platelet-Derived Growth Factor-BB human
Company
MilliporeSigma
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
P3201
Molecular Weight
~25 kDa
Species
human
Molecule Name
ADRA1D, ADRB3, ADRB2
NCBI Full Gene Name
adrenoceptor beta 3, adrenoceptor beta 2, adrenoceptor alpha 1D
NCBI Gene Aliases
ADRA1A, B2AR, ALPHA1, BAR, BETA2AR, ADRA1, ADRB2R, ADRBR, ADRA1R, DAR, dJ779E11.2, BETA3AR
Applications
mammalian cell culture
Format
Lyophilized from a 0.2 µm filtered solution in 30 mM acetic acid
Quantity
10µG, 50µG
Add to Compare List
MilliporeSigma
400 Summit Drive
Burlington
,
Massachusetts
01803
United States
Phone
: (800)-645-5476
(800)-645-5439
Company Profile
Website:
www.SigmaAldrich.com/
Citations (19)
(1)
pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling in living cells
eLife
May 22, 2023
Farahani PE, Yang X, Mesev EV, Fomby KA, Brumbaugh-Reed EH, Bashor CJ, Nelson CM, Toettcher JE.
protein",
FGF4
,
R&D Systems
,Cat #
235-F4-025
, "Peptide, recombinant protein",PDGF-BB,
Millipore Sigma
,Cat #
P3201
, "Peptide, recombinant protein",
VEGF-C
,
R&D Systems
,Cat #
9199-VC-025
, "Chemical compound, drug",
Gefitinib
,
Cell Signaling Technology
(2)
Non-catalytic role of phosphoinositide 3-kinase in mesenchymal cell migration through non-canonical induction of p85β/AP-2-mediated endocytosis
Research Square
January 21, 2023
Hideaki Matsubayashi, Jack Mountain, Tony Yao, Amy Peterson, Abhijit Deb Roy, Takanari Inoue
frozen fibronectin was thawed, the remainder was kept at 4°C. PDGF-BB was purchased from
Sigma-Aldrich
(
P3201
), reconstituted with 4 mM HCl containing 0.1% BSA to obtain 50 μg/mL stock solution, and stored at −20°C.
(3)
Non-catalytic role of phosphoinositide 3-kinase in mesenchymal cell migration through non-canonical induction of p85β/AP-2-mediated endocytosis
bioRxiv
January 4, 2023
Hideaki T. Matsubayashi, Jack Mountain, Tony Yao, Amy F. Peterson, Abhijit Deb Deb Roy, Takanari Inoue
frozen fibronectin was thawed, the remainder was kept at 4°C. PDGF-BB was purchased from
Sigma-Aldrich
(
P3201
), reconstituted with 4 mM HCl containing 0.1% BSA to obtain 50 μg/mL stock solution, and stored at −20°C.
(4)
Rheb regulates nuclear mTORC1 activity independent of farnesylation
Cell Chemical Biology
June 1, 2022
Yanghao Zhong, Xin Zhou, Kun-Liang Guan, Jin Zhang
San Diego respectively to perform experiments. METHOD DETAILS Reagents and Drug Treatment PDGF (
P3201
) was purchased from
Sigma
and was used at 50 ng/mL to stimulate starved cells. Rapamycin (R-5000) was
(5)
Abi1 mediates airway smooth muscle cell proliferation and airway remodeling via Jak2/STAT3 signaling
iScience
February 18, 2022
Wang R, Wang Y, Liao G, Chen B, Panettieri RA Jr, Penn RB, Tang DD.
Ham’s F-12 nutrient mix
,
GIBCO
,11765-047/2187149
Protein A/G Plus-Agarose
,
Santa Cruz
Biotech.,SC-2003 PDGF-BB,
Sigma
,#
P3201
-10UG Lot# SLBW2507 & Lot# SLBZ6214 SDS,
Bio-Rad
,
161-0302
Human STAT3,
Abcam
,
Ab268982
/GR3352364-3 Trypan
(6)
New insights into the clinical and molecular spectrum of the novel CYFIP2-related neurodevelopmental disorder and impairment of the WRC-mediated actin dynamics
Genetics in Medicine
March 1, 2021
Anaïs Begemann et al.
and incubated in medium supplemented with 10 pg/ml of human platelet derived growth factor BB (PDGF) (
P3201
,
Sigma-Aldrich
, St. Louis, Missouri). Migration was assessed by multiframe
live cell imaging
at 37 °C
(7)
Identification of a Dexamethasone Mediated Radioprotection Mechanism Reveals New Therapeutic Vulnerabilities in Glioblastoma
Cancers
January 19, 2021
Aldaz P, Auzmendi-Iriarte J, Durántez M, Lasheras-Otero I, Carrasco-Garcia E, Zelaya MV, Bragado L, Olías-Arjona A, Egaña L, Samprón N,
et al
.
doses ranging from 6−12 Grays.
Dexamethasone
(cat#D4920), temozolomide (cat#
T2577
), human PDGF-BB (cat#
P3201
) and LPS (cat# L5293) were from
Sigma
/
Merck
(Madrid, Spain).
IFNγ
(cat#
300-02
) was from
Peprotech
(London,
(8)
Location-specific inhibition of Akt reveals regulation of mTORC1 activity in the nucleus
Nature communications
November 30, 2020
Zhou X, Zhong Y, Molinar-Inglis O, Kunkel MT, Chen M, Sun T, Zhang J, Shyy JY, Trejo J, Newton AC,
et al
.
made available by direct request from the investigators, as well as through Addgene. Reagents PDGF (
P3201
), insulin (I-5523), IBMX (
I5879
) and Gö6983 (G1918) were purchased from
Sigma
. Nuclear-ID Red (Enzo-52406)
(9)
Enhancer of Zeste Homologue 2 Inhibition Attenuates TGF-β Dependent Hepatic Stellate Cell Activation and Liver Fibrosis
Cellular and molecular gastroenterology and hepatology
January 1, 2019
Martin-Mateos R, De Assuncao TM, Arab JP, Jalan-Sakrikar N, Yaqoob U, Greuter T, Verma VK, Mathison AJ, Cao S, Lomberk G,
et al
.
Houston, TX) (10 mmol/L) and/or TGF-β (240-B; R&D Systems, Minneapolis, MN) (10 ng/mL) and/or PDGF-BB (
P3201
;
Sigma-Aldrich
) (10 ng/mL) for 48 hours. All cell lines were maintained under standard
tissue culture
(10)
Platelet-Derived Growth Factor Subunit B Signaling Promotes Pericyte Migration in Response to Loud Sound in the Cochlear Stria Vascularis
Journal of the Association for Research in Otolaryngology : JARO
August 1, 2018
Hou Z, Wang X, Cai J, Zhang J, Hassan A, Auer M, Shi X.
Cell Migration Assay
, Cell Biolabs, Inc., San Diego, CA, USA) containing PDGF-BB at 2, 4, or 8 ng/ml (
P3201
,
Sigma-Aldrich
, USA). The Boyden chambers were placed in an incubator for 6 h. Migrated
pericytes
passed
(11)
De Novo Truncating Mutations in WASF1 Cause Intellectual Disability with Seizures
The American Journal of Human Genetics
July 1, 2018
Yoko Ito, Keren J. Carss, Sofia T. Duarte, Taila Hartley, Boris Keren, Manju A. Kurian, Isabelle Marey, Perinne Charles, Carla Mendonça, Caroline Nava,
et al.
poly-l-lysine-coated coverslips, and stimulated with
platelet
-derived growth factor (PDGF;
Sigma-Aldrich
,
P3201
) for inducing the formation of
lamellipodia
, as previously described.20 Then cells were fixed, filamentous
(12)
Baicalin inhibits PDGF-BB-induced hepatic stellate cell proliferation, apoptosis, invasion, migration and activation via the miR-3595/ACSL4 axis
International journal of molecular medicine
April 1, 2018
Wu X, Zhi F, Lun W, Deng Q, Zhang W.
were serum-starved in an FBS-free medium for 24 h and then induced with PDGF-BB (10 ng/ml; cat. no.
P3201
;
Sigma-Aldrich
; Merck KGaA, Darmstadt, Germany), following the protocol in a previous study (26). The
(13)
Matrix stiffness regulates migration of human lung fibroblasts
Physiological reports
May 1, 2017
Asano S, Ito S, Takahashi K, Furuya K, Kondo M, Sokabe M, Hasegawa Y.
400 μL DMEM/F‐12 containing 0.1% FBS (2 × 105 cells/mL), and placed in the upper chamber. PDGF‐BB (
P3201
;
Sigma‐Aldrich
) prepared in 0.1% BSA in phosphate‐buffered saline (PBS) dissolved in DMEM/F‐12 containing
(14)
Heparan sulfate 6-
O
-endosulfatases, Sulf1 and Sulf2, regulate glomerular integrity by modulating growth factor signaling
American Journal of Physiology-Renal Physiology
March 1, 2016
Yasutoshi Takashima, Kazuko Keino-Masu, Hiroshi Yashiro, Satoshi Hara, Tomo Suzuki, Toin H. van Kuppevelt, Masayuki Masu, Michio Nagata
(Sigma-Aldrich), subsequently incubated with RPMI-1640 supplemented with 10% FBS that contained 10 ng/ml PDGF-B (
P3201
,
Sigma-Aldrich
), 50 ng/ml VEGF (PMG0111, Biosource), 10 ng/ml transforming GF (TGF)- (240-B/CF, R&D
(15)
PDGF beta targeting in cervical cancer cells suggest a fine-tuning of compensatory signalling pathways to sustain tumourigenic stimulation
Journal of Cellular and Molecular Medicine
February 1, 2015
Oana Mihaela Tudoran et al.
BSA solution for 1 hr and incubated with 100 μl of
cell media
or serial dilutions of PDGFBB protein (
P3201
-10UG;
Sigma-Aldrich
) for 2 hrs followed by 1 hr
incubation
with 100 μl of 0.5 μg/ml PDGFBB detection
(16)
Brivanib attenuates hepatic fibrosis in vivo and stellate cell activation in vitro by inhibition of FGF, VEGF and PDGF signaling
PloS one
January 1, 2014
Nakamura I, Zakharia K, Banini BA, Mikhail DS, Kim TH, Yang JD, Moser CD, Shaleh HM, Thornburgh SR, Walters I,
et al
.
used. To study the effect of brivanib on growth factor signaling pathways, human recombinant PDGF-BB (
P3201
,
Sigma-Aldrich
, St. Louis, MO, USA), VEGF, FGF and TGF-β1 (293-VE, 233-FB and 5036-WN, respectively,
(17)
Micropatterning Different Cell Types with Microarray Amplification of Natural Directional Persistence
Advanced Healthcare Materials
February 1, 2013
Kyu-Shik Mun, Girish Kumar, Carlos C. Co, Chia-Chi Ho
Media (EGM; Catalog No.
CC-3162
) was from
Lonza
. Platelet Derived Growth Factor (PDGF; Catalog No.
P3201
) and Bovine Serum Albumin (BSA; Catalog No. A7906) were from
Sigma-Aldrich
.
Alexa fluor 488 Phalloidin
(18)
P-Rex1 cooperates with PDGFRβ to drive cellular migration in 3D microenvironments
PloS one
January 1, 2013
Campbell AD, Lawn S, McGarry LC, Welch HC, Ozanne BW, Norman JC.
Bioscience.
Recombinant human EGF
(
AF-100-15
) was obtained from
Peprotech
EC Ltd,
recombinant human PDGF-BB
(
P3201
), and NRG1 (SRP3055) were obtained from
Sigma-Aldrich
Ltd. siRNA Treatments Transient
knockdown
of
(19)
Islet grafting and imaging in a bioengineered intramuscular space†
Transplantation
November 15, 2009
Piotr Witkowski et al.
recombinant human VEGF165 (PHG0145, Invitrogen, Carlsbad, USA) and 100 ng/ml recombinant human PDGFbb (
P3201
,
Sigma Aldrich
, St Louis, USA) to cRGDfK alginate, calcium gluconate and sterile distilled water solutions. On
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Platelet-Derived Growth Factor-BB human from MilliporeSigma
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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