Fig 1: March3-deficiency promotes Il-3-triggered expansion of myeloid cells. a March3+/+ or March3−/− Lin− bone marrow cells were treated with Il-3 (5 ng/mL) for 4 days before flow cytometry analysis (left panel) and counting of cell numbers (right histograph). Data shown in the right histographs are mean ± SEM, n = 7. ***P < 0.001; ns not significant. b March3+/+ or March3−/− Lin− bone marrow cells were treated with Il-3 (5 ng/mL) for 4 days. The culture media of the cells were collected for measurement of Tnf-α and Il-6 levels by ELISA. Data shown are mean ± SEM, n = 3. **P < 0.01, ***P < 0.001; ns not significant. c The indicated Lin- bone marrow cells were treated with Il-34 (100 ng/mL) for 4 days before flow cytometry analysis (left panel) and counting of cell numbers (right histograph). Data shown in the right histograph are mean ± SEM, n = 3. ns not significant