Alkaline Phosphatase, Calf Intestinal M1821 from Promega

Product Specs
ItemAlkaline Phosphatase, Calf Intestinal
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberM1821
Conjugate/TagUnconjugated
SpeciesCalf
Concentration1u/µl
Quantity1,000u
Molecule NameAlkaline Phosphatase, Calf Intestinal
Molecular Weight~180kDa

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to catalyze the hydrolysis of 1µmol of 4-nitrophenyl phosphate per minute at 37°C in 1M diethanolamine, 10.9mM 4-nitrophenyl phosphate, 0.5mM MgCl2 (pH 9.8 at 25°C).

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Molecular Phenotyping and Mechanisms of Myocardial Fibrosis in Advanced Chronic Kidney DiseaseKidney360November 1, 2023Gayatri Narayanan, Arvin Halim, Alvin Hu, Keith G. Avin, Tzongshi Lu, Daniel Zehnder, Takashi Hato, Neal X. Chen, Sharon M. Moe, Kenneth Limtreatments containing β-GP also contained 1 unit/ml alkaline phosphatase (ALP, Calf Intestinal; Cat. No. M1821; Promega Corporation, Madison, WI) in the media to hydrolyze β-GP and release phosphate. Starvation

(2) Implications of differential transcription start site selection on chronic myeloid leukemia and prostate cancer cell protein expressioniScienceDecember 22, 2022Arif A. Surani, Keith A. Spriggs, Christoph Ufer, Christos Polytarchou, Cristina Montiel-Duarteendonuclease,Promega,Cat#R6161 Taq DNA polymerase,Promega,Cat#M7841 Alkaline Calf Intestinal Phosphatase,Promega,Cat# M1821 T4 DNA Ligase,Promega,Cat#M1801 TRIzol Reagent,ThermoFisher,Cat#15596026 LiCl Precipitation Solution,ThermoFisher,Cat#AM9480 Chloroform,Sigma-Aldrich,Cat#C2432 30%

(3) CROWN ROOTLESS1 binds DNA with a relaxed specificity and activates OsROP and OsbHLH044 genes involved in crown root formation in riceThe Plant JournalJuly 9, 2022Mathieu Gonin, Kwanho Jeong, Yoan Coudert, Jeremy Lavarenne, Giang Thi Hoang, Martine Bes, Huong Thi Mai To, Marieâ€Rose Ndella Thiaw, Toan Van Do, Daniel Moukouanga, et al.open pB2KSP vector was dephosphorylated using calf intestinal alkaline phosphatase (CIAP) (Promega M1821, Promega, Madison, WI, USA). The pro35S‐CRL1‐Tnos and the pro35S‐AS2‐Tnos cassettes were ligated with

(4) Generation of a CHIP isogenic human iPSC-derived cortical neuron model for functional proteomicsSTAR ProtocolsJune 17, 2022Catarina Dias, Erisa Nita, Jakub Faktor, Lenka Hernychova, Tilo Kunath, Kathryn L. BallNEB). Carry out the reaction at 55°C for 1 h.b.Following plasmid digestion add 1 μL of phosphatase (M1821, Promega) and incubate at 37°C for 1c.Mix the forward and reverse gRNA strands in equal amounts and

(5) Native RNA G quadruplex immunoprecipitation (rG4IP) from mammalian cellsSTAR ProtocolsJune 1, 2022Arif A. Surani, Cristina Montiel-Duarte∗TRIzol Reagent,ThermoFisher,Cat#15596026 ∗Chloroform,Sigma-Aldrich,Cat#C2432 Alkaline Calf Intestinal Phosphatase,Promega,Cat# M1821 Ampicillin sodium salt,Sigma-Aldrich,Cat#A9518 T4 DNA Ligase,Promega,Cat#M1801 RNasin Ribonuclease Inhibitor

(6) Short-term heritable variation overwhelms two hundred generations of mutational variance for metabolic traits in Caenorhabditis elegansEvolutionNovember 1, 2020Lindsay M. Johnson, Olivia J. Smith, Daniel A. Hahn, Charles F. Baerguanosine triphosphates were dephosphorylated with alkaline phosphatase (Promega, Madison, WI; Cat #M1821) according to the manufacturer’s directions to produce the corresponding labelled nucleosides. Uric

(7) GPSeq reveals the radial organization of chromatin in the cell nucleusNature BiotechnologyOctober 1, 2020Gabriele Girelli et al.alkaline phosphatase buffer containing 6 μl of calf intestinal alkaline phosphatase (Promega, cat. no. M1821) for 2 h at 37 °C. Next, we ligated YFISH adapters at a final concentration of 0.2 μM in 300 μl of 1X

(8) High-Resolution In Vivo Identification of miRNA Targets by Halo-Enhanced Ago2 Pull-DownMolecular CellJuly 1, 2020Xiaoyi Li et al.RQ1 RNase-Free DNase,Promega,Cat#M6101 RNase A,Affymetrix,Cat#70194Z "Alkaline Phosphatase, Calf Intestinal (CIAP)",Promega,Cat#M1821 T4 RNA Ligase 1,New England Biolabs,Cat#M0204 T4 Polynucleotide Kinase,New England Biolabs,Cat#M0201 SuperScript III Reverse Transcriptase

(9) G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress GranulesCellApril 16, 2020Yang P, Mathieu C, Kolaitis RM, Zhang P, Messing J, Yurtsever U, Yang Z, Wu J, Li Y, Pan Q, et al.rotating wheel. For RNA detection, RNA-G3BP1 complexes were treated with alkaline phosphatase (Promega, M1821) and RNA was biotinylated using an RNA 3’ End Biotinylation Kit (Pierce) as described below. RNA Binding

(10) A Spontaneous Missense Mutation in Branched Chain Keto Acid Dehydrogenase Kinase in the Rat Affects Both the Central and Peripheral Nervous SystemsPloS oneJanuary 1, 2016Zigler JS Jr, Hodgkinson CA, Wright M, Klise A, Sundin O, Broman KW, Hejtmancik F, Huang H, Patek B, Sergeev Y, et al.analysis. To the aliquots lacking phosphatase inhibitors, Calf Intestinal Alkaline Phosphatase (Promega, M1821) was added according to the manufacturer’s instructions and incubated for 30 minutes at 37°C. Aliquots

(11) RNA regulatory networks diversified through curvature of the PUF protein scaffoldNature communicationsSeptember 14, 2015Wilinski D, Qiu C, Lapointe CP, Nevil M, Campbell ZT, Tanaka Hall TM, Wickens M.unit T4 PNK (NEB M0201s), 100 units RNasin (Promega N2511) and 5 units alkaline phosphatase (Promgea M1821 ) at 37 °C for 20 min shaking every 3 min for 15 s followed by washing 2 × with 1 × PNK+EGTA buffer

(12) An improved malachite green assay of phosphate: Mechanism and applicationAnalytical BiochemistryOctober 18, 2010Juan Feng, Yuan Chen, Jun Pu, Xiaolan Yang, Chun Zhang, Sha Zhu, Yunsheng Zhao, Yonghua Yuan, Huidong Yuan, Fei Liao

(13) The measurement of cyclic nucleotide phosphodiesterase 4 activities via the quantification of inorganic phosphate with malachite greenAnalytica Chimica ActaJanuary 15, 2009Sha Zhu, Zhiyong Gan, Zhirong Li, Yin Liu, Xiaolan Yang, Ping Deng, Yanlin Xie, Mingan Yu, Hong Liao, Yunsheng Zhao, Lina Zhao, Fei Liao

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Alkaline Phosphatase, Calf Intestinal

Alkaline Phosphatase, Calf Intestinal from Promega

Description

Specifications/Features

Promega

Citations (13)

Reviews

Alkaline Phosphatase, Calf Intestinal from Promega

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