Endo Hf P0703S from New England Biolabs

Product Specs
ItemEndo Hf
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberP0703S
FormatConcentration:1,000,000 units/ml
Quantity100000 units
Molecule NameEndo Hf
ActivityUnit Definition: One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 10 µg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 10 µl (10 NEB units = 1 IUB milliunit).
ApplicationsProteomics & Glycomics
Brand/TrademarkNew England Biolabs Inc (NEB)
Temperature-20 C
TypeGlycosidases

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) TMEM106B is a receptor mediating ACE2-independent SARS-CoV-2 cell entryCellAugust 3, 2023Jim Baggen, Maarten Jacquemyn, Leentje Persoons, Els Vanstreels, Valerie E. Pye, Antoni G. Wrobel, Valeria Calvaresi, Stephen R. Martin, Chloë Roustan, Nora B. Cronin, et al.2-1042-025 HisTrap Excel,Sigma-Aldrich,Cat# GE29-0485-86 Endoglycosidase H (Endo Hf),New England Biolabs,Cat# P0703S Tris(2-carboxyethyl)phosphine hydrochloride (TCEP),Fisher Scientific,Cat# 15780329; CAS 5961-85-3 Formic

(2) A structural blueprint for interleukin-21 signal modulationCell ReportsJune 19, 2023Gita C. Abhiraman, Theodora U.J. Bruun, Nathanael A. Caveney, Leon L. Su, Robert A. Saxton, Qian Yin, Shaogeng Tang, Mark M. Davis, Kevin M. Jude, K. Christopher Garciapeptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins" ,, Endo Hf,NEB,Cat#P0703S Diatomaceous earth,Sigma,Cat#D3877 Penicillin-Streptomycin,Gibco,Cat#15-140-163 NheI-HF,NEB,Cat#R3131L 1-Step™ Ultra TMB-ELISA Substrate Solution

(3) In vivo deglycosylation of recombinant glycoproteins in tobacco BY‐2 cellsPlant Biotechnology JournalJune 2, 2023Xavier Herman, Johann Far, Marie Peeters, Loïc Quinton, François Chaumont, Catherine Navarreensure optimal N‐site accessibility. Samples were then incubated for 1 h at 37 °C with EndoH 50 U (P0703; New England Biolabs, Ipswich, MA, USA) or PNGase F 25 U (P0704; New England Biolabs) using the manufacturer's

(4) Decoding murine cytomegalovirusPLOS PathogensMay 12, 2023Manivel Lodha, Ihsan Muchsin, Christopher Jürges, Vanda Juranic Lisnic, Anne L’Hernault, Andrzej J. Rutkowski, Bhupesh K. Prusty, Arnhild Grothey, Andrea Milic, Thomas Hennig, et al.manufacturer’s instructions for one hour at 37°C. A similar protocol was performed for EndoHf (NEB P0703S). ß-actin was used as a housekeeping control and immunoblotting was performed using mouse anti- ß-actin

(5) Accumulated precursors of specific GPI-anchored proteins upregulate GPI biosynthesis with ARV1The Journal of cell biologyMay 1, 2023Liu YS, Wang Y, Zhou X, Zhang L, Yang G, Gao XD, Murakami Y, Fujita M, Kinoshita T.1:500 for IF) were used as secondary antibodies. PNGase F (P0704; New England Biolabs) and Endo Hf (P0703; New England Biolabs) were used for cleavage of N-glycans. Biotin (V900418; Sigma-Aldrich) was used

(6) Pharmacological chaperones restore proteostasis of epilepsy-associated GABA(A) receptor variantsbioRxiv : the preprint server for biologyApril 19, 2023Wang YJ, Seibert H, Ahn LY, Schaffer AE, Mu TW.cell lysates were digested with Endoglycosidase H (endo H) enzyme (New England Biolabs, catalog #: P0703L) with G5 reaction buffer at 37°C according to manufacturer’s instruction and the published procedure

(7) Single-component multilayered self-assembling protein nanoparticles presenting glycan-trimmed uncleaved prefusion optimized envelope trimmers as HIV-1 vaccine candidatesNature CommunicationsApril 8, 2023Yi-Nan Zhang, Jennifer Paynter, Aleksandar Antanasijevic, Joel D. Allen, Mor Eldad, Yi-Zong Lee, Jeffrey Copps, Maddy L. Newby, Linling He, Deborah Chavez, et al.trimming of Env immunogens was performed using Endo-Hf, a fusion of endo H and MBP (NEB, catalog no. P0703L), by mixing 20 μg of Env protein, 2 μl of 10× GlycoBuffer3, 5 μl of endo H, and H2O (if necessary) to

(8) Process development for an effective COVID-19 vaccine candidate harboring recombinant SARS-CoV-2 delta plus receptor binding domain produced by Pichia pastorisScientific ReportsMarch 30, 2023Sibel Kalyoncu, Semiramis Yilmaz, Ayca Zeybek Kuyucu, Dogu Sayili, Olcay Mert, Hakan Soyturk, Seyda Gullu, Huseyin Akinturk, Erhan Citak, Merve Arslan, et al.2 ml of 10× GlycoBuffer3 (1× concentration is 50 mM sodium acetate, pH 6) and 0.5 ml of EndoHf (NEB P0703) were added and completed with dH2O. The reaction mix was incubated for 1 h at 37 °C and analyzed by

(9) Mapping SP-C co-chaperone binding sites reveals molecular consequences of disease-causing mutations on protein maturationNature CommunicationsDecember 1, 2022Kristine F. R. Pobre-Piza, Melissa J. Mann, Ashley R. Flory, Linda M. Hendershotby immunoprecipitation. Samples were split and treated with endoglycosidase H (Endo H) or Endo Hf (NEB, P0702, P0703) to remove glycans or incubated without Endo H as a control, according to the manufacturer’s instructions.

(10) Homodimerized cytoplasmic domain of PD-L1 regulates its complex glycosylation in living cellsCommunications biologyAugust 30, 2022Zhou L, Chai F, He Y, Zhou Z, Guo S, Li P, Sun Q, Zu X, Liu X, Huang Q, et al.Selleck), Tunicamycin (HY-A0098, MCE), PD-1-His protein (ab174035, Abcam), PNGase F (P0704, NEB), Endo H (P0703, NEB), 4-Azido-l-phenylalanine (33173-53-4, SustGreen Tech), BetY (481052-60-2, SustGreen Tech), BrC7K

(11) The endoplasmic reticulum membrane complex promotes proteostasis of GABA(A) receptorsiScienceAugust 19, 2022Whittsette AL, Wang YJ, Mu TW.inhibitor cocktail,Roche,4693159001 n-Dodecyl-B-D-maltoside (DDM),GoldBio,DDM5 Endo Hf enzyme,NEB,P0703L Peptide-N-Glycosidase F (PNGase F) enzyme,NEB,P0704L 40% acrylamide/Bis Solution 29:1,Biorad,1610146 2x

(12) Functional properties of the spike glycoprotein of the emerging SARS-CoV-2 variant B.1.1.529Cell reportsJune 14, 2022Wang Q, Anang S, Iketani S, Guo Y, Liu L, Katsamba PS, Shapiro L, Ho DD, Sodroski JG.Galacto-Star™ Reaction Buffer Diluent with Galacton-Star™ Substrate,ThermoFisher,Cat# T1056 PNGase F,NEB,Cat# P0704L Endo Hf,NEB,Cat# P0703L QuikChange Lightning Site-Directed Mutagenesis Kit,Agilent,"Cat# 210,518" "3, 3′,5,5′-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA

(13) Co-translational biogenesis of lipid droplet integral membrane proteinsJournal of cell scienceMarch 1, 2022Leznicki P, Schneider HO, Harvey JV, Shi WQ, High S.Mix) from PerkinElmer, LipidToxRed stain (H34476) from Thermo Fisher Scientific and EndoH (P0702 and P0703) from New England Biolabs. The hybridoma line producing the monoclonal anti-rhodopsin antibody (1:1000)

(14) Adapted Protocol for Saccharibacteria Cocultivation: Two New Members Join the Club of Candidate Phyla RadiationMicrobiology SpectrumJanuary 10, 2022Ahmad Ibrahim, Mohamad Maatouk, Andriamiharimamy Rajaonison, Rita Zgheib, Gabriel Haddad, Jacques Bou Khalil, Didier Raoult, Fadi Bittardeglycosylation, each 180-μl sample/Saccharibacteria coculture was treated with the Endo Hf kit (catalog number P0703L; New England Biolabs, Evry, France), as follows. Three microliters of each reagent was added to the

(15) Functional differences among the spike glycoproteins of multiple emerging severe acute respiratory syndrome coronavirus 2 variants of concerniScienceNovember 19, 2021Wang Q, Nair MS, Anang S, Zhang S, Nguyen H, Huang Y, Liu L, Ho DD, Sodroski JG.Galacto-Star™ Reaction Buffer Diluent with Galacton-Star™ Substrate,Thermo Fisher,Cat# T1056 PNGase F,NEB,Cat# P0704L Endo Hf,NEB,Cat# P0703L "3, 3’,5 ,5’-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA",Sigma-Aldrich,Cat# T4444 Q5 Site-Directed Mutagenesis Kit

(16) RAB39B-mediated trafficking of the GluA2-AMPAR subunit controls dendritic spine maturation and intellectual disability-related behaviourMolecular PsychiatryNovember 1, 2021Maria Lidia Mignogna et al.syringed eight times. Protein lysates were denatured for 15 min at 55 °C and digested with EndoHf (NEB, #P0703S) or PNGasef (NEB, #P0704S) for 2 h at 37 °C. SDS loading buffer (230 mM Tris-HCl pH 6.8, 6.85% SDS,

(17) An alternative pathway for membrane protein biogenesis at the endoplasmic reticulumCommunications biologyJuly 1, 2021O'Keefe S, Zong G, Duah KB, Andrews LE, Shi WQ, High S.Endo H (respectively, for molecular weight proteins ~10-50 kDa or ~50–100 kDa; New England Biolabs, P0703S or P0702S) where indicated, and denatured for 1 h at 37 °C prior to resolution by SDS-PAGE (16% PAGE,

(18) Small RNAs are modified with N-glycans and displayed on the surface of living cellsCellJune 10, 2021Ryan A. Flynn et al.AM2286 "a2–3,6,8,9 Neuraminidase A",New England Biolabs,Cat# P0722 Endo Hf,New England Biolabs,Cat# P0703 PNGaseF,New England Biolabs,Cat# P0704 Endo F2,New England Biolabs,Cat# P0772 Endo F3,New England Biolabs

(19) Potent SARS-CoV-2 neutralizing antibodies directed against spike N-terminal domain target a single supersiteCell Host & MicrobeMay 1, 2021Gabriele Cerutti et al.Scientific,Cat# PI88946 Pierce™ Fab Preparation Kit,Thermo Scientific,Cat# 44985 EndoHf,NEB,Cat# P0703S Tris Base,Thermo Scientific,Cat# BP152-10 Calcium acetate,Sigma,Cat# 114460-21-8 Sodium Cacodylate,Hampton

(20) POST1/C12ORF49 regulates the SREBP pathway by promoting site-1 protease maturationProtein & cellApril 1, 2021Xiao J, Xiong Y, Yang LT, Wang JQ, Zhou ZM, Dong LW, Shi XJ, Zhao X, Luo J, Song BL.(No. D3571) were from Invitrogen. Blasticidin (No. 60218ES10) was from Yeasen. Endoglycosidase H (No. P0703S) and peptide N-glycosidase F (No. P0704S) were from New England Biolabs. Lipoprotein-deficient serum

Be the First to Write a Review!

Endo Hf

Endo Hf from New England Biolabs

Description

New England Biolabs

Citations (47)

Reviews

Endo Hf from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map