Fig 1: Dot blot confirmation of serum IgG auto-reactivity.Two identified antigens of serum autoantibodies, PTCD2 and ICAM4, were spotted onto nitrocellulose and probed with serum identical to that used on the protein microarrays. Serial dilution with diminishing immunoreactivity confirmed the presence and activity of IgG autoantibodies in human sera.
Fig 2: Differential Expression of PTCD2 and FRMD8 autoantibodies in AD and NDC sera.Microarray fluorescence values reflecting individual serum autoantibody titers demonstrate a difference in the expression of anti-PTCD2 and anti-FRMD8 in AD (n = 50) and NDC (n = 40) sera (a,c). This difference was confirmed in independent dot blots that assessed AD and NDC sera reactivity to purified PTCD2 and FRMD8 protein antigens (b,d).
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