Human M-CSF, premium grade 130-096-485 from Miltenyi Biotec

(1) Caspase-8 activation by cigarette smoke induces pro-inflammatory cell death of human macrophages exposed to lipopolysaccharideCell Death & DiseaseNovember 25, 2023Marta Cristaldi, Marco Buscetta, Maura Cimino, Agnese La Mensa, Maria Rita Giuffrè, Luigi Fiore, Claudia Carcione, Fabio Bucchieri, Francesca Rappa, Claudia Coronnello, et al.Saline w/o Calcium w/o Magnesium (ECB4004L) were purchased from Euroclone (Milan, Italy). Human M-CSF (130-096-493) was purchased from Miltenyi Biotec (Bergisch Gladbach, Germany). The following chemicals were obtained

(2) Quantifying spatial dynamics of Mycobacterium tuberculosis infection of human macrophages using microfabricated patternsCell Reports MethodsNovember 20, 2023Anca F. Savulescu, Nashied Peton, Delia Oosthuizen, Rudranil Hazra, Robert P. Rousseau, Musa M. Mhlanga, Anna K. Coussensserum,Sigma-Aldrich,Cat# H3667 Human GM-CSF,Miltenyi Biotec,Cat# 130-093-864 Human M-CSF,Miltenyi Biotec,Cat# 130-096-485 Recombinant Human Interferon gamma,R&D Systems,Cat# 285-IF-100 Accutase,Sigma-Aldrich,Cat# A6964 TRIzol reagent

(3) Multimodal cartography of human lymphopoiesis reveals B and T/NK/ILC lineages are subjected to differential regulationiScienceOctober 20, 2023Kutaiba Alhaj Hussen, Emna Chabaane, Elisabeth Nelson, Shalva Lekiashvili, Samuel Diop, Seydou Keita, Bertrand Evrard, Aurélie Lardenois, Marc Delord, Els Verhoeyen, et al."Human IL-15, premium grade",Miltenyi Biotech,Cat#130-095-765 "Human M-CSF, premium grade",Miltenyi Biotech,Cat#130-096-492 "Human SCF, premium grade",Miltenyi Biotech,Cat#130-096-695 "Human TPO, premium grade",Miltenyi Biotech,Cat#130-095-752 "Human IL-21, premium grade

(4) Label-free Chemical Characterization of Polarized Immune Cells in vitro and Host Response to Implanted Bio-instructive Polymers in vivo Using 3D OrbiSIMSBio ProtocAugust 5, 2023Waraporn Suvannapruk, Max K. Edney, Leanne E. Fisher, Jeni C. Luckett, Dong-Hyun Kim, David J. Scurr, Amir M. Ghaemmaghami, Morgan R. Alexandernumber: 130-093-919)Macrophage colony-stimulating factor (M-CSF) (Miltenyi Biotech, catalog number: 130-096-493)70% alcohol (any vendor)Optimal cutting temperature compound (OCT) (Agar Scientific, catalog number:

(5) Nanostructured Microparticles Repolarize Macrophages and Induce Cell Death in an In Vitro Model of Tumour-Associated MacrophagesPharmaceuticsJuly 5, 2023Salma Al-Fityan, Britta Diesel, Thorben Fischer, Emmanuel Ampofo, Annika Schomisch, Vida Mashayekhi, Marc Schneider, Alexandra K. Kiemermedium supplemented with 20 ng/mL human macrophage-colony stimulating factor (M-CSF, Miltenyi Biotec, 130-096-492) at 37 °C and 5% CO2 for 5 days. After differentiation, macrophages were left without polarization (M0)

(6) A druggable copper-signalling pathway that drives inflammationNatureMay 1, 2023Solier S, Müller S, Cañeque T, Versini A, Mansart A, Sindikubwabo F, Baron L, Emam L, Gestraud P, Pantoș GD, et al.macrophages, pan monocytes were treated with macrophage colony-stimulating factor (M-CSF, Miltenyi Biotec, 130-096-492, 100 ng ml−1) for 5 days and subsequently, IL-4 (Miltenyi Biotec, 130-093-921, 20 ng ml−1) was added

(7) Ebola virus–like particles reprogram cellular metabolismJournal of Molecular MedicineMay 1, 2023Huaqi Tang, Yasmine Abouleila, Anno Saris, Yoshihiro Shimizu, Tom H. M. Ottenhoff, Alireza Mashaghifactor (GM-CSF; 130–093-864, Miltenyi Biotec) or 50 ng/ml macrophage colony-stimulating factor (M-CSF; 130–096-489, Miltenyi Biotec), respectively [10]. Cells were cultured at 37 °C/5% CO2 in RPMI 1640 medium (31,870,025,

(8) DNA ultra-sensitive quantification, a technology for studying HIV unintegrated linear DNACell Reports MethodsApril 24, 2023Hélène Marie Roux, Suzanne Figueiredo, Lucas Sareoua, Maud Salmona, Juliette Hamroune, Lucie Adoux, Julie Migraine, Allan Hance, François Clavel, Rémi Cheynier, et al.Human IL-7 Premium grade,Miltenyi Biotec,Cat# 130-095-362 Human M-CSF Premium grade,Miltenyi Biotec,Cat# 130-096-489 Benzonase Nuclease,Merck Millipore,Cat# 70746 Dolutegravir,Selleckchem,Cat# S2667 Nevirapine,Selleckchem,Cat#

(9) Targeting neuronal lysosomal dysfunction caused by β-glucocerebrosidase deficiency with an enzyme-based brain shuttle constructNature communicationsApril 12, 2023Gehrlein A, Udayar V, Anastasi N, Morella ML, Ruf I, Brugger D, von der Mark S, Thoma R, Rufer A, Heer D, et al.Fisher Scientific), 100 U/mL penicillin/streptomycin, 50 µM 2-mercaptoethanol, 100 ng/mL M-CSF (Cat# 130-096-492, Miltenyi Biotec), and 25 ng/mL IL-3 (Cat# 130-095-07, Miltenyi Biotec) at 37 °C and 5% CO2. 50% media

(10) Characterization of Anti-Cancer Activities of Violacein: Actions on Tumor Cells and the Tumor MicroenvironmentFrontiers in OncologyMay 11, 2022Charlotte Dahlem, Shilpee Chanda, Jan Hemmer, Hanna S. Schymik, Michael Kohlstedt, Christoph Wittmann, Alexandra K. Kiemersupplemented with 20 ng/ml human recombinant macrophage colony-stimulating factor (M-CSF, Miltenyi #130-096-492) for 6 days. Polarization of HMDMs was performed in vitro: To obtain M1 polarization, media was supplemented

(11) Initial properdin binding contributes to alternative pathway activation at the surface of viable and necrotic cellsEuropean Journal of ImmunologyApril 1, 2022Mieke F. van Essen et al.with 10 ng/mL GM‐CSF; premium grade, 130–093‐868, Miltenyi Biotec) or 100 ng/mL M‐CSF; premium grade, 130‐096‐493, Miltenyi Biotec), respectively. Analysis of differentiation is shown in Supporting information Fig.

(12) The antimicrobial peptide S100A8/A9 produced by airway epithelium functions as a potent and direct regulator of macrophage phenotype and functionThe European respiratory journalApril 1, 2022Skronska-Wasek W, Durlanik S, Le HQ, Schroeder V, Kitt K, Garnett JP, Pflanz S.transferrin, Lonza) with the addition of 100 ng·mL−1 of macrophage colony-stimulating factor (M-CSF) (MILT-130-096-489, Miltenyi Biotec). Cell culture Small airway epithelial cells (SAECs) from non-COPD and COPD individuals

(13) Reprogramming monocyte-derived macrophages through caspase inhibitionOncoImmunologyMarch 7, 2022Paul Chaintreuil et al.a modulation of MDMs. Methods Reagents and antibodies Human CSF-1 was purchased from Miltenyi (130–096-493). Emricasan (IDN-6556) was purchased from Euromedex (S7775-5 mg). Q-VD-OPh was from Clinisciences (A1901-5

(14) Dysregulation of cholesterol homeostasis in human lung cancer tissue and tumour-associated macrophageseBioMedicineOctober 1, 2021Jessica HoppstÃ¤dter, Anna Dembek, Marcus HÃ¶ring, Hanna S. Schymik, Charlotte Dahlem, Afnan Sultan, Natalie Wirth, Salma Al-Fityan, Britta Diesel, Gilles Gasparoni, et al.glutamine) supplemented with 20 ng/ml macrophage-colony stimulating factor (M-CSF, Miltenyi Biotec, #130-096-492) for six days. After differentiation, MDMs were polarized for another 24 hours. To this end, MDM medium

(15) CD276 is an important player in macrophage recruitment into the tumor and an upstream regulator for PAI-1Scientific reportsJuly 21, 2021Durlanik S, Fundel-Clemens K, Viollet C, Huber HJ, Lenter M, Kitt K, Pflanz S.(BEBP02-055Q, Lonza) and differentiated to macrophages by incubation for 7 days with 100 ng/ml M-CSF (130-096-489, Miltenyi Biotec) or GM-CSF (130-093-866, Miltenyi Biotec). Crispr-Cas9 gene editing for HCT116 cells

(16) The HSP GRP94 interacts with macrophage intracellular complement C3 and impacts M2 profile during ER stressCell death & diseaseJanuary 22, 2021Chaumonnot K, Masson S, Sikner H, Bouchard A, Baverel V, Bellaye PS, Collin B, Garrido C, Kohli E.After adhesion, monocytes were cultivated in RPMI 10% FBS with 100 ng/ml GM-CSF or M-CSF (130-093-866, 130-096-492, Miltenyi Biotec, Bergisch Gladbach, Germany) for 6 days, then respectively activated with 20 ng/ml

(17) Infiltration by CXCL10 Secreting Macrophages Is Associated With Antitumor Immunity and Response to Therapy in Ovarian Cancer SubtypesFrontiers in immunologyJanuary 1, 2021Ardighieri L, Missale F, Bugatti M, Gatta LB, Pezzali I, Monti M, Gottardi S, Zanotti L, Bignotti E, Ravaggi A, et al.monocytes were cultured over 7 days in the presence of 100 ng/ml human M-CSF premium grade (Cat. No. 130-096-489, Miltenyi Biotec) to generate macrophages (M0). The medium was not replaced throughout the culture period.

(18) Deletion of lysophosphatidylcholine acyltransferase 3 in myeloid cells worsens hepatic steatosis after a high-fat dietJournal of Lipid ResearchJanuary 1, 2021Thibaut Bourgeois et al.bone marrow cells were implanted in 12-well plates. Cells were treated during 5–7 days with human M-CSF (130-096-492, Miltenyi) until full macrophage differentiation. Isolation of Kupffer cells and primary hepatocytes Mice

(19) Altered iron metabolism in cystic fibrosis macrophages: the impact of CFTR modulators and implications for Pseudomonas aeruginosa survivalScientific reportsJuly 2, 2020Hazlett HF, Hampton TH, Aridgides DS, Armstrong DA, Dessaint JA, Mellinger DL, Nymon AB, Ashare A.heat-inactivated FBS (HI-FBS; Genesee Scientific, cat#25-514H) with 100 ng/ml M-CSF (Miltenyi, cat#130-096-489). Experimental procedure Experiments were performed in RPMI 1640 Glutamax with 50 µg/ml gentamicin

(20) Thioholgamide A, a New Anti-Proliferative Anti-Tumor Agent, Modulates Macrophage Polarization and MetabolismCancers (Basel)May 19, 2020Charlotte Dahlem et al.supplemented with 20 ng/mL human recombinant macrophage colony-stimulating factor (M-CSF, Miltenyi #130-096-492) for 6 days. To polarize the Cancers 2020, 12, 1288 14 of 21 human monocyte-derived macrophages

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2/12/2016Macrophage Differentiation Using M-CSF Max HübnerOur aim was to differentiate macrophages from human PBMCs using M-CSF in different concentrations.

Human M-CSF, premium grade from Miltenyi Biotec

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Human M-CSF, premium grade from Miltenyi Biotec

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