Fig 1: Genetic instability in CD34+ cells grown for 3 days in medium supplemented with recombinant GRP78, CALR, PDIA3 and GPI. (A) ?H2AX foci levels in CD34+ cells (n = 4 patients); Poisson regression: p = 0.0344; Dunnett’s test each p < 0.0001). (B) Exemplary immunofluorescence images of ?H2AX foci (green, Alexa 488) in nuclei (blue, DAPI) of CD34+ cells. Scale bar, 7.5 µm. (C) Numbers of aberrant centrosomes per CD34+ cell (n = 3 patients); Kruskal–Wallis test: p = 0.0249; Wilcoxon two-sample test: each p = 0.1000). (D) Exemplary immunofluorescence images of regular, structural aberrant and numerical aberrant centrosomes (orange, Alexa 555) and microtubules (green, Alexa 488) in nuclei (blue, DAPI) of CD34+ cells. Scale bar, 5 µm. (E) Numbers of aberrant metaphases per CD34+ cell (n = 6 patients); Kruskal–Wallis test: p = 0.0028; Wilcoxon two-sample test: each p = 0.0022. (F) Exemplary aberrant karyotype of a CD34+ cell grown in medium supplemented with recombinant CALR. Arrows point at chromatid breaks chtb(2q) and chtb(4q), respectively. Data in (A + C + E) are presented as means ± SEM.
Supplier Page from Novus Biologicals, a Bio-Techne Brand for Recombinant Human Calreticulin His Protein