COLEC10 (Human) Recombinant Protein (P01) H00010584-P01 from Abnova Corporation

Published Figures

Register or Sign In to View Figures.

PLoS genetics
March 01 2017

Fig 1: CL-L1 regulates cell migration and act as migratory chemoattractant.A. Experimental design for cell migration assay and invasion rate. Agarose spots containing CL-L1 and/or CL-K1 were attached to the glass and HeLa cells seeded. After 24 hours pictures were taking on different spots. The area covered inside the spot by the cells (advance front) was measured and normalised for the length of the perimeter of the spot. B. Representative pictures of agarose spots containing PBS, BSA and CL-L1 with cells invading 48 hours after seeding after. Note how HeLa cells are attracted and invade the agarose sport containing CL-L1. C. Quantification of the invasion ratio. HeLa cells when exposed to CL-L1 cells were more attracted to invade the agarose spots than PBS controls. D. ELISA results in HeLa and HEK293 cell pellets and supernatants after COLEC10 mutant construct transfections. COLEC10 Wild-type (COLEC10WT) and three COLEC10 mutations (COLEC10ArgXTer, COLEC10Gly77Glufs*66 and COLEC10Cys176Trp) cDNAs where cloned and transfected in HEK293 and HeLa cells. Untransfected cells C(-) were used as a control to prove no endogenous CL-L1 was affecting the readings. Concentration of expressed CL-L1 was tested by ELISA in cells extracts and cell supernatant. CL-L1 was found in COLEC10WT and COLEC10Cys176Trp cell extracts in both cell types, but no CL-L1 was expressed after transfecting COLEC10ArgXTer or COLEC10Gly77Glufs*66 constructs or in the untransfected C(-) cells. COLEC10WT and COLEC10Cys176Trp showed very similar levels of CL-L1 expression in HeLa and HEK293 cell types, with slightly higher expression in COLEC10Cys176Trp, (2518 ng/ml HeLa and 1302 ng/ml HEK293) versus COLEC10WT (1823 ng/ml HeLa and 632 ng/ml HEK293). However, the levels of CL-L1 were undetectable in the supernatant of COLEC10Cys176Trp transfections, in contrast with COLEC10WT transfected cells (200 ng/ml HeLa and 390 ng/ml HEK293)
Register or Sign In to View Figures.

PLoS genetics
March 01 2017

Fig 2: New 3MC mutations in COLEC11, MASP1/3 and COLEC10 A-C.Summary of 3MC mutations position in CL-K1, MASP1, MASP3 and CL-L1 proteins. Boxes indicate position of new mutations described in this work. Asterisks indicate already known mutations. D. Pedigrees of families 19 and 25 with COLEC10 mutations. COLEC10 mutations are indicated under carriers and affected patients. E. Chromatogram of new COLEC10 mutations. F. Tridimensional structure by SWISS-MODEL Workspace for Wild-type and Cys176Trp mutated CL-L1. On the left, lateral view of the second helix-loop-helix domain. This domain is part of the C-lectin type domain. On the right, forward view. Arrow indicates the position of the missense change p.Cys176Trp. The structure of CL-L1 c-lectin domain changes in p.Cys176Trp mutants (arrowheads).
Register or Sign In to View Figures.

PLoS genetics
March 01 2017

Fig 3: Cellular and embryonic localisation of CL-L1.A. Immunostaining of ATDC5 cells with the golgi marker 58K and CL-L1. CL-L1 shows localisation with golgi apparatus (white arrow). B. Laminin and CL-L1 coimmunolocalisation. Laminin shows partial cellular immunolocalisiton with CL-L1 around the golgi area (arrows). Scale bar 50 µm C. Laminin and CL-K1 coimmunolocalisation. CL-K1 staining shows a very strong golgi localisation with partial cytoplasmatic laminin colocalisation. D. CL-L1 immunohistochemistry of a 18.5 days postfertilisation mouse embryo. CL-L1 is expressed in the liver (long arrow) and submucosal patal region (short arrows). E. Immunofluorescence showing co-localisation of CL-L1 and Laminin in E13.5 mouse embryos sections. CL-L1 is expressed in the basal membrane of the ephithelium in the palate shelf of the maxilla (arrows). In contrast Laminin expression is present all around the ephitelium membrane. A faint but clear CL-L1 expression is also observed in the cytoplasm of the epithelium and in the mesenchyme of the palate. PSM; rostral extremity of right palatal shelf of maxilla. Scale bar 100 µm.