Recombinant Human IL-12 (p70) (carrier-free) 573004 from BioLegend

Product Specs
ItemRecombinant Human IL-12 (p70) (carrier-free)
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number573004
ApplicationsBioassay
Quantity25 µg
Gene Nametumor necrosis factor
NCBI Gene AliasesCLMF, IL-12A, NFSK, NKSF, NKSF2, NKSF1, CLMF2, P35, IMD29, IMD28, IL-12B
NCBI Full Gene Nameinterleukin 12B, interleukin 12A
FormatThe protein was 0.22µm filtered in PBS, pH 7.2
Molecule NameIL-12 p70
Concentration0.20 mg/mL
SpeciesHuman

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BioLegend

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Phone: 1-877-246-5343 Toll-Free, USA & Canada
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Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Influenza vaccination reveals sex dimorphic imprints of prior mild COVID-19NatureFebruary 23, 2023Rachel Sparks, William W. Lau, Can Liu, Kyu Lee Han, Kiera L. Vrindten, Guangping Sun, Milann Cox, Sarah F. Andrews, Neha Bansal, Laura E. Failla, et al.added 4 hours before harvest. Cytokines were purchased from BioLegend: IL-15 (cat# 570304), IL-12 (cat# 573004), IL-18 (cat# 592104). Flow cytometry a) B cell phenotyping panel including influenza HA probes Thawed

(2) Immunoengineered MXene nanosystem for mitigation of alloantigen presentation and prevention of transplant vasculopathyNano TodayFebruary 1, 2023Weiang Yan, Alireza Rafieerad, Keshav Narayan Alagarsamy, Leena Regi Saleth, Rakesh C. Arora, Sanjiv Dhingrarecombinant human IL-2 (589102, BioLegend). To induce TH1 polarization, 10 ng/mL of recombinant human IL-12 (573002, BioLegend) was added to the culture medium. The co-cultures of HUVECs and human PBMNCs were performed

(3) MHC class II molecules on pancreatic cancer cells indicate a potential for neo-antigen-based immunotherapyOncoImmunologyDecember 31, 2022Renato B. Baleeiro et al.(Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation; #11132D; Invitrogen) and 5 ng/mL human recombinant IL-12 (#573002; BioLegend). Human recombinant IL-2 (#589106; BioLegend) at 30 U/mL and IL-15 (#570304; BioLegend) at

(4) The antimicrobial peptide cathelicidin drives development of experimental autoimmune encephalomyelitis in mice by affecting Th17 differentiationPLoS biologyAugust 1, 2022Smith KJ, Minns D, McHugh BJ, Holloway RK, O'Connor R, Williams A, Melrose L, McPherson R, Miron VE, Davidson DJ, et al.cathelicidin (LL-37). Five days later, cells were washed twice and replated with 50 ng/ml rIL-12 (Biolegend #573002) again with or without LL-37. Three days later, IFN-γ production was assessed by flow cytometry. In vitro

(5) Novel Small Molecule Tyrosine Kinase 2 Pseudokinase Ligands Block Cytokine-Induced TYK2-Mediated Signaling PathwaysFrontiers in ImmunologyMay 20, 2022Yu Zhou et al.by serially-diluted test compounds, and incubated with 40 ng/mL recombinant human IL12 (Biolegend, #573002) for overnight in a humidified, 5% CO2 cell culture incubator at 37°C. The supernatant was then collected

(6) CDK8 Fine-Tunes IL-6 Transcriptional Activities by Limiting STAT3 Resident Time at the Gene LociSSRN Electronic JournalMarch 31, 2022Jonathan Martinez-Fabregas, Luopin Wang, Elizabeth Pohler, adeline cozzani, Stephan Wilmes, Majid Kazemian, Ignacio MoragaStemCell Technologies Cat#70025 Recombinant human Interleukin-2 Novartis Cat#709421 Recombinant human Interleukin-12 Biolegend Cat#573002 Recombinant human Interleukin-1b R&D Systems Cat#201-LB/CF Biological Samples Chemicals, Peptides,

(7) Alcohol Impairs Immunometabolism and Promotes Naïve T Cell Differentiation to Pro-Inflammatory Th1 CD4(+) T CellsFrontiers in immunologyJanuary 1, 2022McTernan PM, Levitt DE, Welsh DA, Simon L, Siggins RW, Molina PE.of soluble anti-CD28 (2.5 μg/ml; catalog # 16-0289-81; ThermoFisher) and IL-12 (25 ng/ml; catalog #573002; Biolegend, San Diego, CA) for 3 days in 37°C incubators with 5% CO2 with and without 50 mM ethanol.

(8) Fabrication of Smart Tantalum Carbide MXene Quantum Dots with Intrinsic Immunomodulatory Properties for Treatment of Allograft VasculopathyAdvanced Functional MaterialsNovember 10, 2021Alireza Rafieerad, Weiang Yan, Keshav Narayan Alagarsamy, Abhay Srivastava, Niketa Sareen, Rakesh C. Arora, Sanjiv DhingraBioLegend). For TH1 polarization, the medium was also supplemented with 10 ng mL−1 recombinant human IL‐12 (573002, BioLegend). The cells were cultured for one week and analyzed using flow cytometry. Flow Cytometry The

(9) Identifying cytokine signaling signatures in primary human Th-1 cells by phospho-proteomics analysisSTAR protocolsJune 18, 2021Martinez-Fabregas J, Pohler E, Moraga I.proteins" ,, Recombinant human interleukin-2,Novartis,Cat#709421 Recombinant human interleukin-12,BioLegend,Cat#573002 MSC2530818,Stratech,Cat#S8387-SEL Recombinant human hyper-interleukin-6,Expressed and purified in

(10) Competitive binding of STATs to receptor phospho-Tyr motifs accounts for altered cytokine responseseLifeApril 19, 2021Stephan Wilmes et al.#709421, 20 ng/ml), anti-IL-4 antibody (10 ng/ml, BD Biosciences, #554481), IL-12 (20 ng/ml, BioLegend, #573002). After 3 days of priming, cells were expanded for another 5 days in the presence of IL-2 (20 ng/ml). Human

(11) CDK8 Fine-Tunes IL-6 Transcriptional Activities by Limiting STAT3 Resident Time at the Gene LociCell reportsDecember 22, 2020Martinez-Fabregas J, Wang L, Pohler E, Cozzani A, Wilmes S, Kazemian M, Mitra S, Moraga I.manufacturer instructions in the presence of 20 ng/mL IL2 (Novartis #709421), 20 ng/mL IL12 (BioLegend, #573002) and 10 ng/mL anti-IL4 (BD Biosciences, #554481). Then, cells were split into three different conditions

(12) Paradoxical Pro-inflammatory Responses by Human Macrophages to an Amoebae Host-Adapted Legionella EffectorCell host & microbeApril 8, 2020Price C, Jones S, Mihelcic M, Santic M, Abu Kwaik Y.570202 Human IL-4,Biolegend,Cat # 574002 Human TNF-α,Biolegend,Cat # 570102 Human IL-12p70,Biolegend,Cat # 573002 M-Per Mammalian Protein Extraction Reagent,Thermo Scientific,Cat # 78505 Koningic acid,Cayman Chemical,Cat

(13) Kinetics of cytokine receptor trafficking determine signaling and functional selectivityeLifeNovember 27, 2019Martinez-Fabregas J, Wilmes S, Wang L, Hafer M, Pohler E, Lokau J, Garbers C, Cozzani A, Fyfe PK, Piehler J, et al.instructions in the presence of IL2 (20 ng/ml, Novartis Cat#709421), IL12 (20 ng/ml, BioLegend, Cat#573002) and anti-IL4 (10 ng/ml, BD Biosciences, Cat#554481). Then, cells were expanded in the presence of IL2

(14) Peptidylarginine deiminases 2 and 4 modulate innate and adaptive immune responses in TLR-7-dependent lupusJCI insightDecember 6, 2018Liu Y, Lightfoot YL, Seto N, Carmona-Rivera C, Moore E, Goel R, O'Neil L, Mistry P, Hoffmann V, Mondal S, et al.

(15) TGFβ Imprinting During Activation Promotes Natural Killer Cell Cytokine HypersecretionCancersNovember 5, 2018Foltz JA, Moseman JE, Thakkar A, Chakravarti N, Lee DA.IL-12, IL-15, and IL-18, primary NK cells were stimulated overnight with 10 ng/mL IL-12 (Biolegend, 573002), 50 ng/mL IL-15 (Biolegend, 570302) and 50 ng/mL IL-18 (Biolegend, 592102) as described and rested

(16) CD4 T-cells regulate angiogenesis and myogenesisBiomaterialsSeptember 1, 2018Brian J. Kwee, Erica Budina, Alexander J. Najibi, David J. Mooneythe respective phenotypes: 5 μg/mL anti-IL-4 (BioLegend #500815), 10 ng/mL human IL-12 (BioLegend #573002), 50 ng/mL human IL-2 for Th1; 5 μg/mL anti-IFN-γ (BioLegend #506513), 25 ng/mL human IL-4 (R&D #204-IL-010),

(17) L-Arginine Modulates T Cell Metabolism and Enhances Survival and Anti-tumor ActivityCellOctober 20, 2016Geiger R, Rieckmann JC, Wolf T, Basso C, Feng Y, Fuhrer T, Kogadeeva M, Picotti P, Meissner F, Mann M, et al.(FCCP),Sigma,Cat#C2920 Antimycin,Sigma,Cat#A8674 Recombinant human interleukin-2,BD Biosciences,Cat#554603 Recombinant human interleukin-12,Biolegend,Cat#573002 Human recombinant interleukin-2 (transfected J588L cell supernatant),In house,N/A FlowCytomix basic

(18) Broad-spectrum antibodies against self-antigens and cytokines in RAG deficiencyThe Journal of clinical investigationNovember 2, 2015Walter JE, Rosen LB, Csomos K, Rosenberg JM, Mathew D, Keszei M, Ujhazi B, Chen K, Lee YN, Tirosh I, et al.catalog BTCYT-204); IFN-ω (Thermo Fisher Scientific; catalog BMS304); or IL-12p70 (BioLegend; catalog 573004) by incubation overnight at 4°C. Plates were then washed in PBS-Tween 0.05%, blocked by incubation with

(19) A technique for the measurement of breakage and repair of DNA alkylated in vivoChemico-biological interactionsDecember 1, 1978Eastman A, Bresnick E.

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Recombinant Human IL-12 (p70) (carrier-free) from BioLegend

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Recombinant Human IL-12 (p70) (carrier-free) from BioLegend

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