Fig 1: Effect of WG on PTH1R. (A) Western blot analysis of PTH1R. (B) Quantitative analysis of PTH1R relative to ß-actin. SW1353 cells were pre-treated with indicated concentration of WG or DXM for 4 h in 0.2% BSA media followed by IL-1ß for 24 h. (C) Western blot analysis of cytosolic and nuclear fractions using HDAC4 antibody, (D) quantitative analysis of cytosolic HDAC4 relative ß-actin, (E) quantitative analysis of nuclear HDAC4 relative to Lamin A/C. SW1353 cells were pre-treated with WG or DXM for 4 h in 0.2% BSA media in the presence or absence of PTH (7–34) followed by IL-1ß for 24 h. (# p < 0.05 and ## p < 0.01 vs. control; ** p < 0.01 vs. IL-1ß; † p < 0.05 vs. WG).