Fig 1: mRNA expression of selected genes in hpGCs. Human primary granulosa cells were isolated from follicular fluids of two groups of IVF patients: (1) young patients (“Young”; 20–35 y) and (2) RA patients (“Aged”; 36–44 y), triggered with hCG. Cells were pooled, incubated for 24 h, and then collected and subjected to qPCR analysis of PEDF, VEGF, FSHR, and AMH expression. Number of pools in each experimental group: n > 10 (two-to-three women per pool; each black circle represents one pool). Genes were calibrated with HPRT-1. Data are presented as mean ± SEM. Results were analyzed by Student’s t-test with Welch’s correction, * pv ? 0.05.
Fig 2: PEDF and VEGF protein in human GCs and in FF. phGCs extracts and FFs of IVF patients: phGCs and FFs of young (“Young”; 20–35 y; N = 10) and RA patients (“Aged”; 36–44 y; N = 11) were collected individually and subjected to specific ELISA assay kits. (A,B) The level of PEDF in GCs extracts (A) and in their corresponding FFs (B). (C) PEDF concentration ratio, calculated by dividing the level of PEDF in FFs by its level in GCs. (D) The level of VEGF in FFs. (E) PEDF/VEGF ratio in FFs. Each black circle represents one patient. Data are presented as mean ± SEM. Results were analyzed by Student’s t-test with Welch’s correction. * pv < 0.05; ** pv < 0.01; *** pv < 0.001. (F) Spearman correlation analysis of PEDF/VEGF ratio to patients’ age.
Fig 3: mRNA expression of selected genes in mouse granulosa cells. Mouse granulosa cells were isolated from individual young (“Young”; 6–8 weeks old) and RA (“Aged”; 40–46 weeks old) ICR female mice, primed for 48 h with PMSG (5IU or 7.5 IU, respectively). Cells were then subjected to qPCR analysis of PEDF, VEGF, FSHR, and AMH expression. Number of mice in each group: n > 15; each black circle represents one mouse. Data are presented as mean ± SEM. Results were analyzed by Student’s t-test with Welch’s correction, * pv ? 0.05.
Supplier Page from Abcam for Recombinant Human PEDF protein