Fig 1: GDNF stimulated axon outgrowth from DRGs into Matrigel in the 3D explant model. NFG, GDNF, or BDNF was added to the culture media. (A) Immunofluorescent staining of mouse DRG in a Matrigel drop. Axons were visualized with antibody staining against NF200 (red fluorescence), nuclei counterstained with DAPI, and Nestin-positive cells (green fluorescence). Scale bar: 250 µm. (B) A graph presenting the quantification of axon growth. Data are presented as mean ± standard deviation (SD). * p < 0.05, n = 4.
Fig 2: GDNF and NGF stimulated migration of Nestin-green fluorescent protein (GFP) cells from DRGs into the Matrigel in a 3D explant model. (A) A microphotograph presents the DRG from Nestin-GFP mouse in a drop of Matrigel. Nuclei are counterstained with DAPI, and green fluorescence corresponds with Nestin-GFP-expressing cells. Scale bar: 250 µm. (B) A graph presenting the average number of double-positive (GFP and DAPI) cells that migrated into the Matrigel. Data are presented mean ± standard deviation (SD), * p < 0.05, n = 4.
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