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BglII
BglII
Supplier Images
BglII
BglII from New England Biolabs
(1)
Write a review
Citations:
(12)
Supplier Page
Supplier Page from
New England Biolabs for
BglII
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Description
BglII
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
BglII
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0144M
Quantity
10000 units
Concentration
50,000 units/ml
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
5'-A/GATCT-3'
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (12)
(1)
Topoisomerase II deficiency leads to a postreplicative structural shift in all Saccharomyces cerevisiae chromosomes
Scientific Reports
December 1, 2021
Jessel Ayra-Plasencia, Cristina Ramos-Pérez, Silvia Santana-Sosa, Oliver Quevedo, Sara Medina-Suárez, Emiliano Matos-Perdomo, Marcos Zamora-Dorta, Grant W. Brown, Michael Lisby, Félix Machín
(see below). In case of subsequent overnight treatment of the agarose plugs with 250 units of
BglII
(
R0144M
,
New England Biolabs
),
Proteinase K
was first inactivated with 1 mg/ml Pefabloc (
11429868001
,
Roche
). PFGEs
(2)
An optimized protocol for the generation of HBV viral antigen-specific T lymphocytes from pluripotent stem cells
STAR protocols
March 19, 2021
Haque M, Xiong X, Lei F, Das JK, Song J.
Pittsburgh),N/A BamH1/EcoR1,
New England Biolabs
,CAT#
R0136T
/
R0101T
EcoRI
/
BglII
,
New England Biolabs
,CAT#
R0101T
/
R0144M
,, "Buffer, media, and reagents","Buffer, media, and reagents","Buffer, media, and reagents" ,, α-
MEM
,
Invitrogen
,CAT#
(3)
Lineage dynamics of the endosymbiotic cell type in the soft coral Xenia
Nature
June 25, 2020
Minjie Hu, Xiaobin Zheng, Chen-Ming Fan, Yixian Zheng
rotation (950 rpm) in a Thermomixer. To digest the crosslinked genomic DNA, 30 μl of 50 U/μl
BglII
(
NEB
R0144M
) was added to the mixture and incubated overnight at 37 °C with rotation at 950 rpm in a Thermomixer. Fill
(4)
Three New Integration Vectors and Fluorescent Proteins for Use in the Opportunistic Human Pathogen Streptococcus pneumoniae
Genes (Basel)
May 22, 2019
Lance E. Keller, Anne-Stéphanie Rueff, Jun Kurushima, Jan-Willem Veening
(5)
Functional characteristics of novel pancreatic Pax6 regulatory elements
Human Molecular Genetics
October 1, 2018
Adam Buckle, Ryu-suke Nozawa, Dirk A Kleinjan, Nick Gilbert
before Triton X-100 was added to 2% for 1 h. 1000 U of concentrated BglII restriction enzyme (
NEB
Cat#
R0144M
) was added for overnight digestion at 37°C and 1200 rpm. Digestion efficiency was analysed on 5 μl pre-
(6)
Lamins Organize the Global Three-Dimensional Genome from the Nuclear Periphery
Molecular Cell
September 1, 2018
Xiaobin Zheng, Jiabiao Hu, Sibiao Yue, Lidya Kristiani, Miri Kim, Michael Sauria, James Taylor, Youngjo Kim, Yixian Zheng
incubated at 37°C for 10 min. To digest the crosslinked
chromatin
, 30 μl 50 units/μl
BglII
enzyme (
NEB
,
R0144M
) were added to the suspension. The enzyme reactant was incubated overnight at 37°C. Biotin filling
(7)
Single-cell Hi-C for genome-wide detection of chromatin interactions that occur simultaneously in a single cell
Nature Protocols
December 1, 2015
Takashi Nagano, Yaniv Lubling, Eitan Yaffe, Steven W Wingett, Wendy Dean, Amos Tanay, Peter Fraser
Nature America, Inc. All rights reserved. protocol •
BglII
(50,000 U/ml;
New England Biolabs
, cat. no.
R0144M
);
NEBuffer 3
(10×) supplied with the enzyme. Alternative enzymes can be used instead, as discussed in
(8)
Dynamic enhancer–gene body contacts during transcription elongation
Genes & Development
October 1, 2015
Kiwon Lee, Chris C.-S. Hsiung, Peng Huang, Arjun Raj, Gerd A. Blobel
and quenched with 0.25 M glycine for 5 min. We used highly concentrated
BglII
(
New England Biolabs
,
R0144M
) and T4 ligase (
New England Biolabs
,
M0202L
). Proximity
ligation
products were quantified in triplicate
(9)
Exploring CTCF and cohesin related chromatin architecture at HOXA gene cluster in primary human fibroblasts
Science China Life Sciences
September 1, 2015
Xing Wang, Miao Xu, GuangNian Zhao, GuoYou Liu, DeLong Hao, Xiang Lv, DePei Liu
(3C) The 3C
assay
was performed as previously described [18]. Briefly, restricted enzyme
BglII
(
NEB
,
R0144M
) was used to digest the 1.7% formaldehyde-cross-linked nuclei from FsF or HLF cells. The efficiency
(10)
CTCF Controls
HOXA
Cluster Silencing and Mediates PRC2-Repressive Higher-Order Chromatin Structure in NT2/D1 Cells
Molecular and Cellular Biology
October 15, 2014
Miao Xu, Guang-Nian Zhao, Xiang Lv, Guoyou Liu, Lily Yan Wang, De-Long Hao, Junwen Wang, De-Pei Liu, Chih-Chuan Liang
modifications. Briefly, 1.7% formaldehyde-cross-linked
chromatin
was digested with
BglII
(product code
R0144M
;
NEB
) overnight. The subsequent
ligation
was performed with DNA concentrations of less than 2.5 ng/l
(11)
Targeted Mutagenesis in Atlantic Salmon (Salmo salar L.) Using the CRISPR/Cas9 System Induces Complete Knockout Individuals in the F0 Generation
PLoS ONE
September 25, 2014
Rolf B. Edvardsen, Sven Leininger, Lene Kleppe, Kai Ove Skaftnesmo, Anna Wargelius
(12)
Fusion of fluorescent protein to puromycin N-acetyltransferase is useful in Drosophila Schneider S2 cells expressing heterologous proteins
Cytotechnology
March 1, 2013
Kotomi Nagahashi, Kazuo Umemura, Naohiro Kanayama, Takayuki Iwaki
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Reviews
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(1)
10/2/2015
High Quality Restriction Enzyme for Cloning
Prabhakaran Kumar
I used this BglII restriction enzyme along with EcoRV for the cloning of my gene of interest into a plasmid vector. Subsequently, I also used this enzyme for colony screening. This enzyme completely ...
Read Review
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BglII from New England Biolabs
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