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BglII
BglII
Supplier Images
BglII
BglII from New England Biolabs
(2)
Write a review
Citations:
(48)
Supplier Page
Supplier Page from
New England Biolabs for
BglII
Get Pricing
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
BglII
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0144S
Quantity
2000 units
Concentration
10 u/µL
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
A/GATCT
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (48)
(21)
Pitfalls in AR42J-model of cerulein-induced acute pancreatitis
PLOS ONE
January 25, 2021
Marcus Hollenbach, Sebastian Sonnenberg, Ines Sommerer, Jana Lorenz, Albrecht Hoffmeister
purified. The inserts and a pEGFP-N1 vector (6085–1, Clontech, California, USA) were digested by Bgl-II (
R0144S
,
NEB
, Massachusetts, USA) and
EcoRI
(
R0101S
,
NEB
) and the vector was dephosphorylated (Shrimp alkaline
(22)
Multiplex T-cell Stimulation Assay Utilizing a T-cell Activation Reporter-based Detection System
BIO-PROTOCOL
January 20, 2021
Laurie G Landry, Sarah E Mann, Amanda M Anderson, Maki Nakayama
(
NEB
, catalog number:
R0685S
) i.
SalI
(
NEB
, catalog number:
R0138S
) j.
BglII
(
NEB
, catalog number:
R0144S
) k.
MluI-HF
(
NEB
, catalog number:
R3198S
) l.
SbfI
(
NEB
, catalog number:
R0642S
) 34. Primers (Integrated
(23)
PKCγ-Mediated Phosphorylation of CRMP2 Regulates Dendritic Outgrowth in Cerebellar Purkinje Cells
Molecular neurobiology
December 1, 2020
Winkler SC, Shimobayashi E, Kapfhammer JP.
linearized for 1 h at 37 °C in the appropriate buffer using
BglII
(
New England BioLabs
, Ipswich, USA;
R0144S
) and
SalI-HF
(
New England BioLabs
, Ipswich, USA;
R3138S
) restriction enzymes. The linearized vector
(24)
Constitutively bound CTCF sites maintain 3D chromatin architecture and long-range epigenetically regulated domains
Nature Communications
December 1, 2020
Amanda Khoury et al.
further 60 min
incubation
at 37 °C with shaking.
Chromatin
was digested overnight with 750U
BglII
(#
R0144S
,
New England Biolabs
) at 37 °C with shaking. Ends were repaired and marked with biotin-14-dATP using
(25)
TREM2 Modulation Remodels the Tumor Myeloid Landscape Enhancing Anti-PD-1 Immunotherapy
Cell
August 20, 2020
Molgora M, Esaulova E, Vermi W, Hou J, Chen Y, Luo J, Brioschi S, Bugatti M, Omodei AS, Ricci B,
et al
.
Performance,
GE Healthcare
,Cat #
17–0403-01
XhoI
,
New England Biolabs
,Cat #
R0146S
BglII
,
New England Biolabs
,Cat #
R0144S
NheI-HF
,
New England Biolabs
,Cat #
R3131S
Expi293TM Expression Medium,
Thermo Scientific
,Cat #
A1435101
"
Lipoprotein, high density from human plasma
(26)
Extracellular vesicles as a platform to study cell-surface membrane proteins
Methods
August 1, 2020
Vincent Delauzun, Beatrice Amigues, Anais Gaubert, Philippe Leone, Magali Grange, Laurent Gauthier, Alain Roussel
[23] was used to produce EVs. This vector was digested with
EcoRI-HF
(
NEB
#
R3101S
) and
BglII
(
NEB
#
R0144S
) restriction enzymes in order to remove the mcherry gene out of the vector. Linearized vector was then separated
(27)
Rapid and Efficient Synthetic Assembly of Multiplex Luciferase Reporter Plasmids for the Simultaneous Monitoring of Up to Six Cellular Signaling Pathways
Current Protocols in Molecular Biology
June 26, 2020
Alejandro Sarrion‐Perdigones, Yezabel Gonzalez, Koen J.T. Venken
ampicillin or 100 μg mL−1 spectinomycin. Restriction enzymes:
BglII
(
New England Biolabs
, cat. no.
R0144S
),
BsaI
(
New England Biolabs
, cat. no.
R0535S
),
BsmBI
(
New England Biolabs
, cat. no.
R0580S
),
EcoRI-HF
(28)
Human Papillomavirus Quasivirus Production and Infection of Primary Human Keratinocytes
Current Protocols in Microbiology
June 1, 2020
Samuel S. Porter, Alison A. McBride
NucleoBond Xtra Maxi Plus EF
Maxi kit (
Machery Nagel
#740426.50) BglII restriction enzyme (
NEB
#
R0144S
) NEB
CutSmart Buffer
(
NEB
#
B7204S
)
Gel Loading Dye, Purple (6X
) (
NEB
#
B7024S
) SeaKem GTG Agarose
(29)
Proteome Instability Is a Therapeutic Vulnerability in Mismatch Repair-Deficient Cancer
Cancer cell
March 16, 2020
McGrail DJ, Garnett J, Yin J, Dai H, Shih DJH, Lam TNA, Li Y, Sun C, Li Y, Schmandt R,
et al
.
CT-M7243
Bortezomib
,
Selleck Chemicals
,Cat# S10135MG TrypLE Express,
Invitrogen
,Cat#
12605010
Bgll,
New England Biolabs
,Cat#
R0144S
Nsil,
New England Biolabs
,Cat#
R0127S
BseR1,
New England Biolabs
,Cat#
R0581S
Critical Commercial
Assays
,Critical
(30)
Identification of serine residues in the connexin43 carboxyl tail important for BCR-mediated spreading of B-lymphocytes
Journal of cell science
March 10, 2020
Pournia F, Dang-Lawson M, Choi K, Mo V, Lampe PD, Matsuuchi L.
258-
BglII
/
BglII
-EGFP and 307-
BglII
/
BglII
-EGFP (confirmed by
sequencing
) were digested by
BglII
RE (
R0144S
,
New England BioLabs
, lpswich, MA, USA) and re-ligated using
T4 DNA Ligase
(
15224041
,
Invitrogen
). Thus,
(31)
Combinatorial action of NF–Y and TALE at embryonic enhancers defines distinct gene expression programs during zygotic genome activation in zebrafish
Developmental Biology
March 1, 2020
William Stanney, Franck Ladam, Ian J. Donaldson, Teagan J. Parsons, René Maehr, Nicoletta Bobola, Charles G. Sagerström
,
ThermoFisher Scientific
,
10001D
NotI
,
New England Biolabs
,
R0189S
XhoI
,
New England Biolabs
,
R0146S
BglII
,
New England Biolabs
,
R0144S
Critical Commercial
Assays
,Critical Commercial
Assays
,Critical Commercial
Assays
mMESSAGE mMACHINE SP6 Transcription Kit
(32)
A Well-Mixed E. coli Genome: Widespread Contacts Revealed by Tracking Mu Transposition
Cell
February 13, 2020
David M. Walker, Peter L. Freddolino, Rasika M. Harshey
,
New England Biolabs
,
M0315S
HinP1,HinP1,
New England Biolabs
,
R0124S
AlwNI
,
AlwNI
,
New England Biolabs
,
R0514S
BglII
,
BglII
,
New England Biolabs
,
R0144S
T4 DNA ligase,T4 DNA ligase,
New England Biolabs
,
M0202S
Phusion Polymerase,Phusion Polymerase,
New England Biolabs
(33)
Targeted De Novo Centromere Formation in Drosophila Reveals Plasticity and Maintenance Potential of CENP-A Chromatin
Developmental cell
February 10, 2020
Palladino J, Chavan A, Sposato A, Mason TD, Mellone BG.
Recombinant Proteins","Chemicals, Peptides, and Recombinant Proteins"
KpnI
,
NEB
,Cat#
R0142S
BglII
,
NEB
,Cat#
R0144S
XhoI
,
NEB
,Cat#
R0146S
HhaI
,
NEB
,Cat#
R0139S
HpaII
,
NEB
,Cat#
R0171S
Taq DNA polymerase,
NEB
,Cat#
M0273L
T4
(34)
Buffalo early pregnancy biomarker coding sequence cloning and partial length expression in E. coli after codon optimization
Heliyon
November 27, 2019
Shree Vidhya Srinivasan, Jyotirmoy Ghosh, Sayed Basha Nazar, Kajal Sankar Roy
restriction enzymes (
New England Biolabs
, UK) in 20 μL reaction volume viz., BamH I (
R0136S
), Bgl II (
R0144S
), Eag I (
R0505S
),
PmeI
(
R0560S
), Sal I (
R0138S
) and Xho I (
R0146S
). Based on
electrophoresis
, sizes
(35)
A PhoA-STII Based Method for Efficient Extracellular Secretion and Purification of Fab from Escherichia coli
BIO-PROTOCOL
September 20, 2019
Ziyan Wang, Yang Gao, Manyu Luo, Cedric Cagliero, Hua Jiang, Yueqing Xie, Jianwei Zhu, Huili Lu
Biotechnology, catalog number: EC1002) 11. Bgl II (concentration: 10 units/μl) (
NEB
, catalog number:
R0144S
), store at -20 °C 12. Nde I (concentration: 20 units/μl ) (
NEB
, catalog number:
R0111S
), store at -20
(36)
Three New Integration Vectors and Fluorescent Proteins for Use in the Opportunistic Human Pathogen Streptococcus pneumoniae
Genes (Basel)
May 22, 2019
Lance E. Keller, Anne-Stéphanie Rueff, Jun Kurushima, Jan-Willem Veening
(37)
Inhibiting the Evolution of Antibiotic Resistance
Molecular Cell
January 3, 2019
Mark N. Ragheb, Maureen K. Thomason, Chris Hsu, Patrick Nugent, John Gage, Ariana N. Samadpour, Ankunda Kariisa, Christopher N. Merrikh, Samuel I. Miller, David R. Sherman,
et al.
Recombinant Proteins" ,,
BamHI-HF
,
NEB
,
R3136S
HindIII-HF
,
NEB
,
R3104S
NotI-HF
,
NEB
,
R3189S
BglII
,
NEB
,
R0144S
NheI
,
NEB
,
R0131S
XhoI
,
NEB
,
R0146S
4-Nitroquinoline N-oxide,Sigma,N8141
Gibson Assembly Master Mix
,
NEB
,
E2611S
Phusion
(38)
A B-Cell-Specific Enhancer Orchestrates Nuclear Architecture to Generate a Diverse Antigen Receptor Repertoire
Molecular cell
January 3, 2019
Barajas-Mora EM, Kleiman E, Xu J, Carrico NC, Lu H, Oltz EM, Murre C, Feeney AJ.
37%,Fisher Scientific,BP531–25 RPMI Medium 1640,Gibco,11875–093
BglII
(Restriction Endonuclease),
NEB
,
R0144S
Blasticidin,Lifetech
Invitrogen
,
R21001
NlaIII
(Restriction Endonuclease),
NEB
,
R0125S
Critical Commercial
(39)
Epstein-Barr virus BORF2 inhibits cellular APOBEC3B to preserve viral genome integrity
Nature microbiology
January 1, 2019
Cheng AZ, Yockteng-Melgar J, Jarvis MC, Malik-Soni N, Borozan I, Carpenter MA, McCann JL, Ebrahimi D, Shaban NM, Marcon E,
et al
.
GGC TGA CGC GGA-3’ and 5’-GAC TTC TAG AAG AGG CAC AGT ACT TAT ATA CTC-3’, digested with
BglII
(
NEB
R0144S
) and
XbaI
, and ligated into similarly cut pCMV-3F. All new plasmid constructions were confirmed by restriction
(40)
Therapeutic effect of targeted Fas-expressing adenoviruses method combining γδ T cells in a mouse model of human ovarian carcinoma
Oncology Letters
December 13, 2017
Dingyuan Zeng, Jiajing Lin, Hongying He, Guangping Tan, Ying Lan, Fuyan Jiang, Shuting Sheng
enzyme (cat. no. R0195L), EcoRI restriction enzyme (cat. no. R0101S), BglII restriction enzyme (cat. no.
R0144S
), SalI restriction enzyme (cat. no.
R3138S
), SacI restriction enzyme (cat. no.
R3156S
),
NotI
restriction
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Reviews
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(1)
4/24/2018
Diagnostic Restriction Mapping Of Plasmid Construct With BglII Restriction Enzme
Triveni Mahendrappa
I have constructed a plasmid called pCVD442_operon B of a size 18992 bp size. This plasmid was prepped from carrier E. coli strains and digested with BglII restriction enzyme from NEB. Restriction ...
Read Review
(1)
10/2/2015
High Quality Restriction Enzyme for Cloning
Prabhakaran Kumar
I used this BglII restriction enzyme along with EcoRV for the cloning of my gene of interest into a plasmid vector. Subsequently, I also used this enzyme for colony screening. This enzyme completely ...
Read Review
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BglII from New England Biolabs
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