Cultrex 3-D Culture Matrix RGF Basement Membrane Extract from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCultrex 3-D Culture Matrix RGF Basement Membrane Extract
CompanyR&D Systems, a Bio-Techne Brand
Price Pricing Info Supplier Page View Company Product Page
Catalog Number3445-005-01
Quantity5 mL
SpeciesMulti-Species
Brand/TrademarkR&D Systems, a Bio-Techne Brand
ApplicationsBioactivity
Format14 - 16 mg/mL
TypeBasement Membrane Extract
StorageProduct is stable for a minimum of 3 months from date of shipment when stored at -20 °C in a manual defrost freezer. For optimal stability, store at -80 °C. Avoid freeze-thaw cycles. Storage Buffer: Dulbecco’s Modified Eagle’s medium without phenol red, with 10 µg/ml gentamicin sulfate. Sterility Testing: PathClear® - Negative by PCR test for mycoplasma; 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses. No bacterial or fungal growth detected after incubation at 37 °C for 14 days following USP sterility testing guidelines

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Therapeutic effect and transcriptome-methylome characteristics of METTL3 inhibition in liver hepatocellular carcinomaCancer Cell InternationalNovember 27, 2023Qingbin Liu, Jingjing Qi, Weiyang Li, Xinchen Tian, Jiaqi Zhang, Fen Liu, Xiulian Lu, Hengchang Zang, Chenqiao Liu, Changlin Ma, et al.HepG2 cells were seeded as 1 × 104 cells/well in DMEM medium containing 1 mg/ml Cultrex matrix (R&D systems, 3445–005-01) and cultured for 5 days to allow spheroid formation. A serial dilution of STM2457 (DMSO solvent control,

(2) Estrogen receptor alpha regulates uterine epithelial lineage specification and homeostasisiScienceSeptember 15, 2023Rizo JA, Davenport KM, Winuthayanon W, Spencer TE, Kelleher AM.another round of centrifugation, the epithelium pellet was resuspended in 80% Cultrex (R&D Systems, 3445-005-01) and 20% organoid expansion media (Table S3). EEO were passaged (1:3 ratio) every 7-10 days. To passage

(3) Linear ubiquitination of PTEN impairs its function to promote prostate cancer progressionOncogeneOctober 1, 2022Guo Y, He J, Zhang H, Chen R, Li L, Liu X, Huang C, Qiang Z, Zhou Z, Wang Y, et al.

(4) Implanted synthetic cells trigger tissue angiogenesis through de novo production of recombinant growth factorsProceedings of the National Academy of Sciences of the United States of AmericaSeptember 20, 2022Chen G, Levin R, Landau S, Kaduri M, Adir O, Ianovici I, Krinsky N, Doppelt-Flikshtain O, Shklover J, Shainsky-Roitman J, et al.were seeded at 10,000 cells/well on top of a growth factor–reduced basement membrane extract (GFR BME; 3445–005-01, R&D Systems)–coated Angiogenesis μ-Slide (Ibidi). The cells were applied with the same treatments mentioned

(5) Organ-Specific, Fibroblast-Derived Matrix as a Tool for Studying Breast Cancer MetastasisCancersJuly 2, 2021Jensen ARD, Horton ER, Blicher LH, Pietras EJ, Steinhauer C, Reuten R, Schoof EM, Erler JT.in 35 mm plastic dishes. Dishes were also coated with Matrigel (Cultrex 3-D culture matrix cat. no. 3445-005-01, lot no. 1519214, R&D Systems, MN, USA), which was diluted in serum-free media (1:1000) to obtain 15

(6) Limited inhibition of multiple nodes in a driver network blocks metastasiseLifeMay 11, 2021Yesilkanal AE, Yang D, Valdespino A, Tiwari P, Sabino AU, Nguyen LC, Lee J, Xie XH, Sun S, Dann C, et al.dye other,"BME (3-D Culture Matrix Reduced Growth Factor Basement Membrane Extract, PathClear)",Trevigen,3445-005-01,"Lot No 37353J16, lot concentration: 15.51 mg/ml" "peptide, recombinant protein",EGF (human),Bio-Techne,236-EG-01M, "software,

(7) Sprouty4 negatively regulates ERK/MAPK signaling and the transition from in situ to invasive breast ductal carcinomaPloS oneJanuary 1, 2021Brock EJ, Jackson RM, Boerner JL, Li Q, Tennis MA, Sloane BF, Mattingly RR.

(8) A new level of in vivo singlet molecular oxygen luminescence measurementsPhotodiagnosis and photodynamic therapyMarch 1, 2020Pfitzner M, Preuß A, Röder B.

(9) Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid cultureNature communicationsDecember 11, 2019Giobbe GG, Crowley C, Luni C, Campinoti S, Khedr M, Kretzschmar K, De Santis MM, Zambaiti E, Michielin F, Meran L, et al.

(10) Natural Histogel-Based Bio-Scaffolds for Sustaining Angiogenesis in Beige Adipose TissueCellsNovember 18, 2019Di Somma M, Schaafsma W, Grillo E, Vliora M, Dakou E, Corsini M, Ravelli C, Ronca R, Sakellariou P, Vanparijs J, et al.

(11) Smac mimetics LCL161 and GDC-0152 inhibit osteosarcoma growth and metastasis in miceBMC cancerSeptember 14, 2019Shekhar TM, Burvenich IJG, Harris MA, Rigopoulos A, Zanker D, Spurling A, Parker BS, Walkley CR, Scott AM, Hawkins CJ.

(12) Human mesenchymal stem cells secrete hyaluronan-coated extracellular vesiclesMatrix biology : journal of the International Society for Matrix BiologyDecember 1, 2017Arasu UT, Kärnä R, Härkönen K, Oikari S, Koistinen A, Kröger H, Qu C, Lammi MJ, Rilla K.

(13) PEDF increases the tumoricidal activity of macrophages towards prostate cancer cells in vitroPloS oneJanuary 1, 2017Martinez-Marin D, Jarvis C, Nelius T, de Riese W, Volpert OV, Filleur S.

(14) Live-Cell Imaging of Protease Activity: Assays to Screen Therapeutic ApproachesMethods in molecular biology (Clifton, N.J.)January 1, 2017Chalasani A, Ji K, Sameni M, Mazumder SH, Xu Y, Moin K, Sloane BF.

(15) In vitro differentiation of human amniotic epithelial cells into insulin-producing 3D spheroidsInternational journal of immunopathology and pharmacologySeptember 1, 2015Okere B, Alviano F, Costa R, Quaglino D, Ricci F, Dominici M, Paolucci P, Bonsi L, Iughetti L.

(16) Silencing β3 Integrin by Targeted ECO/siRNA Nanoparticles Inhibits EMT and Metastasis of Triple-Negative Breast CancerCancer researchJune 1, 2015Parvani JG, Gujrati MD, Mack MA, Schiemann WP, Lu ZR.

(17) Tumor-induced pressure in the bone microenvironment causes osteocytes to promote the growth of prostate cancer bone metastasesCancer researchJune 1, 2015Sottnik JL, Dai J, Zhang H, Campbell B, Keller ET.

(18) Organotypic culture of untransformed and tumorigenic primary mammary epithelial cellsCold Spring Harbor protocolsMay 1, 2015Jechlinger M.

(19) Survival Outcome and EMT Suppression Mediated by a Lectin Domain Interaction of Endo180 and CD147Molecular cancer research : MCRMarch 1, 2015Rodriguez-Teja M, Gronau JH, Minamidate A, Darby S, Gaughan L, Robson C, Mauri F, Waxman J, Sturge J.

(20) AMPK reverses the mesenchymal phenotype of cancer cells by targeting the Akt-MDM2-Foxo3a signaling axisCancer researchSeptember 1, 2014Chou CC, Lee KH, Lai IL, Wang D, Mo X, Kulp SK, Shapiro CL, Chen CS.

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Cultrex 3-D Culture Matrix RGF Basement Membrane Extract from R&D Systems, a Bio-Techne Brand

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R&D Systems, a Bio-Techne Brand

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Cultrex 3-D Culture Matrix RGF Basement Membrane Extract from R&D Systems, a Bio-Techne Brand

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