AvrII from New England Biolabs

Product Specs
ItemAvrII
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR0174L
Concentration4 u/µL
Quantity500 units
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-20 C

Sequence

C/CTAGG

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Changes in searching behaviour of CSL transcription complexes in Notch active conditionsLife Sci AllianceDecember 14, 2023Sarah Baloul, Charalambos Roussos, Maria Gomez-Lamarca, Leila Muresan, Sarah BrayTTTCTAGAGACAGCCGGAAATCTCGAGCGTGG for Hairless::Halo. Plasmids and HaloTag PCR products were digested using AvrII (R0174S; New England Biolabs) and MluI (R0198S; NEB) for Su(H)::Halo, or BglII (R0144L; NEB) and XbaI (R0145S;

(2) S RNA Intergenic Deletions Drive Viral Interference during Arenavirus InfectionsbioRxivNovember 7, 2023Matthew Hackbart, Carolina B. Lópezinclude the full-length GPC and an mCherry gene replacing the NP gene by digestion with AvrII (NEB, R0174S), then insertion gene fragments with NEBuilder HIFI DNA Assembly Mix (NEB, E5520S). For transfection

(3) The Effect of Select SARS-CoV-2 N-Linked Glycan and Variant of Concern Spike Protein Mutations on C-Type Lectin-Receptor-Mediated InfectionVirusesSeptember 9, 2023Arjan Bains, Wenyan Guan, Patricia J. LiWangHindIII-HF (New England Biolabs Cat # R3104S, Ipswich, MA, USA) and AvrII (New England Biolabs Cat # R0174S, Ipswich, MA, USA). To verify the presence of asparagine-to-glutamine spike mutations, the vectors were

(4) AP profiling resolves co-translational folding pathway and chaperone interactions in vivobioRxivSeptember 8, 2023Xiuqi Chen, Christian M. KaiserSacI (NEB, R3156S) to prevent exoIII digestion on the arrest peptide side and then with AvrII (NEB, R0174S) to allow for exoIII digestion towards the candidate protein. Linearized dsDNA was purified and quantified

(5) How chromosomal translocations arise to cause cancer: Gene proximity, trans-splicing, and DNA end joiningiScienceJune 16, 2023Patrick Streb, Eric Kowarz, Tamara Benz, Jennifer Reis, Rolf Marschalekhour at 37°C while shaking at 900 rpm. A total of 400 U restriction enzyme (for AFF1 and MLL: AvrII (R0174L, NEB); for KMT2B and CCND3: HindIII (R3104, NEB) was added and incubated overnight at 37°C while shaking

(6) Structure, sequon recognition and mechanism of tryptophan C-mannosyltransferaseNature Chemical BiologyMay 1, 2023Joël S. Bloch, Alan John, Runyu Mao, Somnath Mukherjee, Jérémy Boilevin, Rossitza N. Irobalieva, Tamis Darbre, Nichollas E. Scott, Jean-Louis Reymond, Anthony A. Kossiakoff, et al.into Pichia pastoris For each mutant, 15 µg of plasmid DNA was linearized overnight using AvrII (NEB, R0174S) then purified by ethanol precipitation. This DNA was transformed into electrocompetent GS115 P. pastoris

(7) Systematically attenuating DNA targeting enables CRISPR-driven editing in bacteriaNature CommunicationsFebruary 8, 2023Daphne Collias, Elena Vialetto, Jiaqi Yu, Khoa Co, Éva d. H. Almási, Ann-Sophie Rüttiger, Tatjana Achmedov, Till Strowig, Chase L. Beiselthe 1% agarose gel following the same conditions as described above for Cas9. AvrII (NEB, Cat. no. R0174L) was used to evaluate precise editing for the RT used in Fig. 1d. BsgI (NEB, Cat. no. R0559L) was used

(8) Prediction of designer-recombinases for DNA editing with generative deep learningNature CommunicationsDecember 27, 2022Lukas Theo Schmitt, Maciej Paszkowski-Rogacz, Florian Jug, Frank Buchholzlibraries were digested with the restriction enzymes NdeI and AvrII (NEB catalog number R0111S and R0174S), which will only cut in non-recombined plasmids. The digested DNA was then desalted with a MF-Membrane

(9) Protocol for production and expression of chimeric bovine-human monoclonal antibodiesSTAR ProtocolsDecember 16, 2022Behnaz Heydarchi, Damian F.J. PurcellRestriction enzyme,New England Biolabs,Cat# R3131LL AvrII Restriction enzyme,New England Biolabs,Cat# R0174L T4 DNA ligase,Thermo Fisher Scientific,Cat# EL0012 ExpiFectamine™ 293 Transfection Kit,Thermo Fisher Scientific

(10) Broad and ultra-potent cross-clade neutralization of HIV-1 by a vaccine-induced CD4 binding site bovine antibodyCell Reports MedicineMay 17, 2022Behnaz Heydarchi et al.Restriction enzyme,New England Biolabs,Cat# R3131LL AvrII Restriction enzyme,New England Biolabs,Cat# R0174L ExpiFectamine™ 293 Transfection Kit,Thermofisher,Cat# A14524 Protein G Agarose Fast Flow,Millipore,Cat#

(11) A Zika virus mutation enhances transmission potential and confers escape from protective dengue virus immunityCell ReportsApril 12, 2022Jose Angel Regla-Nava et al.Triton X-100,Sigma,Cat. #X100-100ML RNeasy Mini Kit,Qiagen,Cat. #73404 AvrII,(New England BioLabs),Cat. #R0174S SphI,(New England BioLabs),Cat. #R0182S NaeI,(New England BioLabs),Cat. #R0190S ClaI,(New England BioLabs

(12) miR‐200a/b/‐429 downregulation is a candidate biomarker of tumor radioresistance and independent of hypoxia in locally advanced cervical cancerMolecular OncologyMarch 1, 2022Anja Nilsen et al.sequence with the restriction enzymes Nhe1 (R3131S; New England Biolabs, Ipswich, MA, USA) and AvrII (R0174S; New England Biolabs). Lentivirus was generated by co‐transfection of packaging plasmids and the pLenti

(13) A bipartite element with allele-specific functions safeguards DNA methylation imprints at the Dlk1-Dio3 locusDevelopmental cellNovember 22, 2021Aronson BE, Scourzic L, Shah V, Swanzey E, Kloetgen A, Polyzos A, Sinha A, Azziz A, Caspi I, Li J, et al.(NEB, R0101) and Sall (NEB, R0138) and amplicon −3 was digested with XhoI (NEB, R0146) and AvrII (NEB, R0174). To insert four guides, the vector was digested with XbaI, amplicon −1 was digested with XbaI and MfeI,

(14) Methylation of the tyrosine hydroxylase gene is dysregulated by cocaine dependence in the human striatumiScienceOctober 22, 2021Kathryn Vaillancourt, Gang G. Chen, Laura Fiori, Gilles Maussion, Volodymyr Yerko, Jean-FranÃ§ois ThÃ©roux, Carl Ernst, Benoit LabontÃ©, Erin Calipari, Eric J. Nestler, et al.identified in the topo TA cloning vector were released from the vector with a double digestion using AvrII (R0174S) and SpeI (R0133S-New England Biolabs) enzymes and gel purification (QIAquick Gel Extraction Kit, Qiagen,

(15) Unraveling the hidden role of a uORF-encoded peptide as a kinase inhibitor of PKCsProceedings of the National Academy of SciencesOctober 5, 2021Divya Ram Jayaram et al.(SI Appendix, Table S2). The resulted amplicon was then digested by SacI and AvrII (no. R0156S, no. R0174S, New England Biolabs, Inc. [NEB]) and cloned into the corresponding sites of the WT plasmid to generate

(16) Development of a pan-neuronal genetic driver in Aedes aegypti mosquitoesCell Reports MethodsJuly 26, 2021Zhilei Zhao, David Tian, Carolyn S. McBridelinearized with restriction enzymes NsiI-HF (New England Biolabs #R3127S) and AvrII (New England Biolabs #R0174S).[2]Syt1 left homology arm from ORL genomic DNA (NCBI: LOC5565901) with final seven Syt1 codons included

(17) Precise genome engineering in Drosophila using prime editingProceedings of the National Academy of SciencesJanuary 14, 2021Justin A. Bosch, Gabriel Birchak, Norbert Perrimon132775) and gel purified. pNos-Cas9attB (56) was digested with XbaI/AvrII (New England Biolabs; R0145, R0174) to remove Cas9 sequences and the backbone fragment was gel purified. PE2 coding sequence was inserted

(18) Comparison of Two Strategies to Generate Antigen-Specific Human Monoclonal Antibodies: Which Method to Choose for Which Purpose?Frontiers in immunologyJanuary 1, 2021Ehlers AM, den Hartog Jager CF, Kardol-Hoefnagel T, Katsburg MMD, Knulst AC, Otten HG.restriction enzyme, R0553L, New England BioLabs, Ipswich, Massachusetts, USAvrII restriction enzyme, R0174L, New England BioLabs, Ipswich, Massachusetts, UST4 Polynucleotide kinase, M0201L, New England BioLabs,

(19) Integrative vectors for regulated expression of SARS-CoV-2 proteins implicated in RNA metabolismWellcome Open ResearchNovember 3, 2020Stefan Bresson, Nic Robertson, Emanuela Sani, Tomasz W Turowski, Vadim Shchepachev, Michaela Kompauerova, Christos Spanos, Aleksandra Helwak, David Tollerveygene inserts, 1 µg of pUC containing the relevant gene was initially digested with AvrII (NEB, Cat No. R0174S) in the same reaction conditions followed by purification. The 5’ overhang, encoding the stop codon,

(20) Generation of a Tumor-Specific Chemokine Gradient Using Oncolytic Vesicular Stomatitis Virus Encoding CXCL9Molecular therapy oncolyticsMarch 27, 2020Eckert EC, Nace RA, Tonne JM, Evgin L, Vile RG, Russell SJ.Thermo Fisher Scientific). PCR products were digested at 37°C for 16 h. Digestions consisted of PCR, AvrII (catalog number R0174S; NEB), SbfI (catalog number R0642S; NEB), and buffer B (BB5; Thermo Fischer Scientific). Primer sequences

Be the First to Write a Review!

AvrII

AvrII from New England Biolabs

Sequence

New England Biolabs

Citations (25)

Reviews

AvrII from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map