PE Streptavidin from BioLegend

Product Specs
ItemPE Streptavidin
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number405203
Quantity100 µg
ApplicationsFlow Cytometry (FC), Intracellular Staining for Flow Cytometry (ICFC)
Concentration0.20 mg/mL
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Conjugate/Tag/LabelPE
SpeciesHuman, Mouse, Rat, All Species
TypeStreptavidin
TargetBiotin

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Two-dose “extended priming” immunization amplifies humoral immune responses by synchronizing vaccine delivery with the germinal center responsebioRxivNovember 24, 2023Sachin H. Bhagchandani, Leerang Yang, Laura Maiorino, Elana Ben-Akiva, Kristen A. Rodrigues, Anna Romanov, Heikyung Suh, Aereas Aung, Shengwei Wu, Anika Wadhera, et al.preincubated with streptavidin (30 min at molar ratio of 1:4 at 25°C) conjugated to phycoerythrin (405203, BioLegend) and/or BV421 (405226, BioLegend). Flow cytometry was carried out on a BD LSR Fortessa or

(2) The effect of an exopolysaccharide probiotic molecule from Bacillus subtilis on breast cancer cellsFrontiers in OncologyNovember 23, 2023Mai R. Nguyen, Emily Ma, Debra Wyatt, Katherine L. Knight, Clodia Osipo400211), biotin anti-mouse IgG2a antibody (Biolegend, Cat# 407103), PE Streptavidin (Biolegend, Cat# 405203). Live/Dead Fixable Aqua Stain Kit was used purchased from Invitrogen (Cat# L34957). Western antibodies

(3) Colitis-induced upregulation of tumor necrosis factor receptor-2 (TNFR2) terminates epithelial regenerative signaling to restore homeostasisiScienceOctober 20, 2023Zohreh Sharifkhodaei, Cambrian Y. Liu, Nandini Girish, Ying Huang, Shivesh Punit, M. Kay Washington, D. Brent Polkantibodies, and donkey anti-goat Alexa Fluor 647 (Invitrogen #A21447), Streptavidin-PE (Biolegend #405203) secondary antibodies were used. After washes, cells were analyzed on a BD LSR II. Ultracomp eBeads

(4) Global analysis of HLA-A2 restricted MAGE-A3 tumor antigen epitopes and corresponding TCRs in non-small cell lung cancerTheranosticsAugust 6, 2023Bei Zhang, Zhiyao Ren, Jianfu Zhao, Yue Zhu, Boya Huang, Chanchan Xiao, Yan Zhang, Jieping Deng, Lipeng Mao, Lei Tang, et al.set as negative control. FACS sorting of antigen-specific CD8+ T cells Streptavidin (BioLegend Cat#405203, US) conjugated fluorophore was added in the peptide-exchanged monomer formed in the above steps for

(5) Novel CRISPR-Associated Gene-Editing Systems Discovered in Metagenomic Samples Enable Efficient and Specific Genome EngineeringThe CRISPR JournalJune 1, 2023Rebecca C. Lamothe, Meghan D. Storlie, Diego A. Espinosa, Rachel Rudlaff, Patrick Browne, Jason Liu, Andres Rivas, Audra Devoto, Jennifer Oki, Ashcon Khoubyari, et al.resuspended in FACs buffer containing 1:400 dilution of phycoerythrin (PE)-streptavidin (Biolegend No. 405203) and other monoclonal antibodies of interest and incubated at 4°C for 1 h. Cells were washed twice with

(6) Identification of fatty acid amide hydrolase as a metastasis suppressor in breast cancerNature communicationsMay 30, 2023Tundidor I, Seijo-Vila M, Blasco-Benito S, Rubert-Hernández M, Adámez S, Andradas C, Manzano S, Álvarez-López I, Sarasqueta C, Villa-Morales M, et al.1:400) and TER119 (Thermo Fisher Scientific #13-5921-82, dilution 1:100) + PE Streptavidin (Biolegend #405203, dilution 1:300) to exclude endothelial and hematopoietic lineages (Lin+ cells) from the analysis. Data

(7) 19n01, a broadly neutralizing antibody against omicron BA.1, BA.2, BA.4/5, and other SARS-CoV-2 variants of concerniScienceApril 21, 2023Melissa García-Vega, Edgar A. Melgoza-González, Sofía Hernández-Valenzuela, Diana Hinojosa-Trujillo, Mónica Reséndiz-Sandoval, Mara Anais Llamas-Covarrubias, Martín Loza-López, Olivia Valenzuela, Alan Soto-Gaxiola, Miguel A. Hernández-Oñate, et al.and recombinant proteins" ,, APC Streptavidin,Biolegend,Cat# 405207 PE Streptavidin,Biolegend,Cat# 405203 "SARS-CoV-2 Spike protein RBD, Omicron Variant, His Tag (BA.1/Omicron)",GenScript,Cat# Z03728 "SARS-CoV-2

(8) Inactivated vaccine-elicited potent antibodies can broadly neutralize SARS-CoV-2 circulating variantsNature CommunicationsApril 17, 2023Yubin Liu, Ziyi Wang, Xinyu Zhuang, Shengnan Zhang, Zhicheng Chen, Yan Zou, Jie Sheng, Tianpeng Li, Wanbo Tai, Jinfang Yu, et al.(Biolegend, 356408, Clone M-T271), streptavidin-APC (Biolegend, 405207) and streptavidin-PE (Biolegend, 405203). The CD3−CD8−CD14−CD19+CD27+Spike-PE+Spike-APC+ B cells were quantified using a CytoFLEX LX (Beckman

(9) Bispecific GPC3/PD‑1 CAR‑T cells for the treatment of HCCInternational journal of oncologyApril 1, 2023Li D, Qin J, Zhou T, Li Y, Cheng X, Chen Z, Chen J, Zheng WV.protein L (cat. no. M00097; GenScript) and then stained with streptavidin-PE at 37°C for 10 min (cat. no. 405203; BioLegend, Inc.). Flow cytometry data were acquired on a Gallios flow cytometer (Beckman Coulter, Inc.)

(10) Insufficient epitope-specific T cell clones are responsible for impaired cellular immunity to inactivated SARS-CoV-2 vaccine in older adultsNature AgingMarch 13, 2023Chanchan Xiao, Zhiyao Ren, Bei Zhang, Lipeng Mao, Guodong Zhu, Lijuan Gao, Jun Su, Jiezhou Ye, Ze Long, Yue Zhu, et al.peptide-exchanged monomer (produced in-house)53 was mixed with 3.3 µl of PE streptavidin (BioLegend, 405203) on a new plate and incubated on ice in the dark for 30 minutes. Then, 2.4 µl of blocking solution (1.6

(11) Efficient clinical-grade γ-retroviral vector purification by high-speed centrifugation for CAR T cell manufacturingMolecular Therapy - Methods & Clinical DevelopmentMarch 9, 2023Leila Mekkaoui, Jose G. Tejerizo, Sara Abreu, Lydie Rubat, Aleksandra Nikoniuk, William Macmorland, Claire Horlock, Sofia Matsumoto, Sarah Williams, Koval Smith, et al.(Thermo Fisher Scientific, cat. no. A-21241) and phycoerythrin-conjugates streptavidin (BioLegend, cat. no. 405203), respectively, along with a viability dye for live cell gating (fixable viability stain 780; BD, cat.

(12) Screening self-peptides for recognition by mouse alloreactive CD8+ T cells using direct ex vivo multimer stainingSTAR ProtocolsMarch 1, 2023Eric T. Son, Moumita Paul-Heng, Mario Leong, Chuanmin Wang, Alexandra E. Hill, Martina Denkova, Anthony W. Purcell, Nicole A. Mifsud, Alexandra F. SharlandPercoll™ PLUS,Cytiva,Cat#17-5445-01 Synthetic peptides,GL Biochem Shanghai Ltd,Custom order PE Streptavidin,BioLegend,Cat#405203 ∗QuickSwitch™ Quant H-2 Kb Tetramer Kit-PE,MBL International,Cat#TB-7400-K1 ∗BioLegend Flex-T™ H-2Kb

(13) Activation-induced cytidine deaminase expression by thymic B cells promotes T-cell tolerance and limits autoimmunityiScienceJanuary 20, 2023Lombard-Vadnais F, Chabot-Roy G, Zahn A, Rodriguez Torres S, Di Noia JM, Melichar HJ, Lesage S.L2654 Papain,Worthington Biochemical,Cat# LS00319 RPMI 1640 Medium,Gibco,Cat# 11875-093 Streptavidin,BioLegend,Cat# 405203 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, Blood glucose

(14) Flow cytometric protocol to characterize human memory B cells directed against SARS-CoV-2 spike protein antigensSTAR protocolsDecember 16, 2022Weskamm LM, Dahlke C, Addo MM.Streptavidin,Biolegend,Cat#405237 PE-Cy7 Streptavidin,Biolegend,Cat#405206 PE Streptavidin,Biolegend,Cat#405203 BV711 Streptavidin,Biolegend,Cat#405241 BV421 Streptavidin,Biolegend,Cat#405225 BIO200 - BIO-200

(15) Lymphatic-preserving treatment sequencing with immune checkpoint inhibition unleashes cDC1-dependent antitumor immunity in HNSCCNature CommunicationsDecember 1, 2022Robert Saddawi-Konefka et al.Biotin H-2 K(b) OVA Monomer (Biolegend 280051) paired with PE-streptavidin or APC-streptavidin (Biogened 405203 or 405207, respectively) or the H-2Kb MuLV p15E Tetramer-KSPWFTTL-PE (MBL TB-M507-1) was used according

(16) T cells specific for α-myosin drive immunotherapy-related myocarditisNatureNovember 1, 2022Axelrod ML, Meijers WC, Screever EM, Qin J, Carroll MG, Sun X, Tannous E, Zhang Y, Sugiura A, Taylor BC, et al.hours at 18°C. Tetramers were produced by adding 2.1 μL of Streptavidin-PE antibody (BioLegend, cat# 405203) and incubating for at least 1 hour at 4°C. Cells were first stained with Zombie Violet (BioLegend,

(17) Mechanism of activation of porcine dendritic cells by an α-D-glucan nanoparticle adjuvant and a nanoparticle/poly(I:C) combination adjuvantFrontiers in ImmunologySeptember 5, 2022Juan F. Hernandez-Franco, Shaojun Xie, Jyothi Thimmapuram, Darryl Ragland, Harm HogenEsch(CTLA-4)-mouse IgG (Fc) fusion protein (#501-030; Ancell, Bayport, MN) followed by streptavidin-PE (#405203; BioLegend), and anti-SLA-DR-FITC (clone 2E9/13; Bio-Rad) for 45 min at 4°C. Flow cytometry was performed

(18) Potent neutralizing anti-SARS-CoV-2 human antibodies cure infection with SARS-CoV-2 variants in hamster modeliScienceAugust 19, 2022Imbrechts M, Maes W, Ampofo L, Van den Berghe N, Calcoen B, Van Looveren D, Kerstens W, Rasulova M, Vercruysse T, Noppen S, et al.(Biolegend, 363016), FITC anti-human CD3 antibody (Biolegend, 300306) and PE streptavidin (Biolegend, 405203) for 25 min on ice. Subsequently, the stained cells were washed twice with FACS buffer and single-cell

(19) Adoptive Cellular Therapy with Autologous Tumor-Infiltrating Lymphocytes and T-cell Receptor–Engineered T Cells Targeting Common p53 Neoantigens in Human Solid TumorsCancer Immunology ResearchAugust 3, 2022Sanghyun P. Kim et al.under UV-light. Following peptide exchange, 10 μL of fluorescently labeled Streptavidin-PE (catalog no. 405203) and Streptavidin-APC (catalog no. 405207, both from BioLegend) were added to saturation in 2 μL increments

(20) Multiepitope supramolecular peptide nanofibers eliciting coordinated humoral and cellular antitumor immune responsesScience advancesJuly 22, 2022Wu Y, Wen H, Bernstein ZJ, Hainline KM, Blakney TS, Congdon KL, Snyder DJ, Sampson JH, Sanchez-Perez L, Collier JH.GK1.5, BioLegend), CD44 (APC-Cy7, clone IM7, BD), PD-1 (APC, clone RMP1-30, BD), and streptavidin-PE (405203, BioLegend). TFH cells were defined as FVD−CD3e+CD4+CD44+PD-1+CXCR5+. To determine antigen-specific

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8/26/2021Bright Fluorochrome Works Great Dimi ComfuOne of the brightest flurochrome-strept conjugates. We routinely use this for our tetramer stainings for cell lines or primarty cells.

PE Streptavidin from BioLegend

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BioLegend

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PE Streptavidin from BioLegend

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