At Atlas Antibodies, producing highly characterized antibodies and working extensively with validation and reproducibility has always been of top priority. You can trust that our antibodies are specific and perform consistently each time you use them.
We welcome and take active part in the development of comprehensive guidelines for antibody validation as requested by the research community. To our extensive product data, we are further adding a layer of formal validation to our antibodies, following the guidelines proposed by Uhlén et al in Nature Methods (September, 2016). Our strategy is to focus on four of the suggested pillars for antibody validation: Genetic Strategies, Orthogonal Strategies, Independent Antibody Strategies and Expression of Tagged Proteins.
In IHC we validate by orthogonal methods by comparing the protein expression to RNA levels and compare independent antibodies directed towards different parts the same proteins.
At Atlas Antibodies, each new antibody lot is tested against its reference lot to make sure that new lots meet the same specificity critera in each application. This is how we guarantee lot-to-lot reproducibility.