The effective use of antibodies in research assays has always depended on multiple factors that determine the accuracy and precision of the component reagents. While researchers began to limit their testing novel antibodies as commercialized antibodies became increasingly available, a re-evaluation of this policy has recently occurred.
As a producer and supplier of many cited antibodies and immunotechnologies, scientists at EMD Millipore have worked extensively with researchers who are concerned about their data interpretation and publication. In this article important considerations in choosing antibodies is discussed and some of the key reviewer comment trends are addressed.
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