Immunophenotyping by multicolor flow cytometry is a powerful diagnostic tool to assess the status of a patient’s immune system, e.g. for immune monitoring and complementary research in clinical trials or the detection of minimal residual disease after treatment for leukemia. Other possible applications include the quality control of cellular therapy products or preclinical research, e.g. mouse xenograft models.
Having reliable, consistently performing antibodies and dyes is crucial to all the aforementioned applications. This poster presents an improved ready-to-use seven color immunophenotyping cocktail, made up of Miltenyi Biotec REAfinity™ antibodies, for the differentiation of nine leukocyte subsets. REAfinity™ are recombinant antibodies of human IgG1 isotype with a mutated region within the Fc portion, which prevents Fcγ receptor binding. They are characterized by high antigen specificity and consistent quality compared to conventional hybridoma-derived monoclonal antibodies.
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