Chromium Fixed RNA Profiling (Gene Expression Flex) offers comprehensive scalable solutions to measure gene expression in single cell and nuclei suspensions that are fixed with formaldehyde. This protocol outlines fixation of blood and isolation of peripheral blood mononuclear cells (PBMCs) and leukocytes from the fixed blood for use with the Chromium Fixed RNA Profiling workflow. Storage recommendations for the PBMCs isolated from fixed blood and post-storage processing conditions are also provided. An overview of data derived from fixed PBMCs and leukocytes used with the Chromium Fixed Profiling workflow is also shown. Download this protocol to learn more.