Optimization of iPSC Culture Protocol Using High Quality Growth Factors and Cytokines
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Optimization of iPSC Culture Protocol Using High Quality Growth Factors and Cytokines

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Cytokines and growth factors constitute a diverse group of proteins essential for intercellular communication. Two cytokines that are a key focus for in vitro cell culture are fibroblast growth factor 2 (FGF-2) and transforming growth factor β1 (TGF-β1). These cytokines are used in precise concentrations for maintaining induced pluripotent stem cells (iPSCs) in their pluripotent state. However, achieving the desired efficacy of these cytokines and growth factors in an in vitro setting has been challenging due to issues like thermostability, leading to a short half-life and necessitating frequent media changes, which are resource intensive.

In this application note, we present data on the utilization and optimization of Sartorius Research Use Only (RUO) Cytokines and Growth Factors for iPSC culture. Our goal is to sustain pluripotency and proliferative potential while reducing the need for daily media replenishment. We assess this by analyzing crucial cell surface markers, morphological indicators, and growth patterns using advanced tools like the iQue® Advanced Flow Cytometer and the Incucyte® Live-Cell Analysis System, showcasing the practicality of Sartorius RUO Growth Factors and Cytokines in iPSC culture maintenance. Download now to learn more.

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