Tumor progression and response to treatment are influenced by the behavior of tumor cells and their interactions with the surrounding microenvironment. Traditionally, the diagnosis and treatment of tumors have been based on a limited number of biomarkers. However, by examining the entire transcriptome of a tumor within its tissue context, valuable insights that can aid in the development of new diagnostics and treatments can be obtained. Most biopsies are preserved using FFPE, which damages RNA and makes transcriptome profiling more challenging. A recent study used Visium Spatial Gene Expression for FFPE, which reveals tumorigenic profiles in triple-positive ductal carcinoma in situ (TP DCIS) of the breast FFPE sections. Download this Data Spotlight now to learn more about the study as well as the integration of spatial data with snRNA-seq to accurately map specific cell types within the tissue microenvironment.