Real-time PCR systems are required to quantitatively determine the levels of gene expression in the cells or tissues. We use PCR cyclers also to determine the levels of human-specific Alu repeats within the DNA purified from animals bearing human tumor xenografts. In combination with a standard curve, this approach allow us to determine the levels of tumor cell dissemination in cancer metastasis models. The CFX Connect Real-Time PCR Systems (##1855201 or 1855200) are designed to detect 1 target or 2 targets simultaneously per well of a 96-well plate. We purchased the CFX Connect Real-Time PCR Detection System with a starter package (#1855200), containing SYBR Green Supermix, 96-well PCR plates and plate seals, which is a highly recommended option.
We do a lot of NK cell detection. We wanted to test a few CD56 antibodies and find the "best" one which would give us the easiest resolution of NK cells from other lymphocytes (see my other reviews). This product was chosen because PE-Cy7 is a bright fluorochrome and is available on our cytometer.