In our lab we study the proliferation of transferred T cells in the mouse model. To do so, we use CD45.2 recipient mice and CD45.1 donor mice. We need an antibody that works well to stain only the recipient mouse cells to differentiate them from the donor mouse cells. This antibody works well to do this.
Real-time PCR systems are required to quantitatively determine the levels of gene expression in the cells or tissues. We use PCR cyclers also to determine the levels of human-specific Alu repeats within the DNA purified from animals bearing human tumor xenografts. In combination with a standard curve, this approach allow us to determine the levels of tumor cell dissemination in cancer metastasis models. The CFX Connect Real-Time PCR Systems (##1855201 or 1855200) are designed to detect 1 target or 2 targets simultaneously per well of a 96-well plate. We purchased the CFX Connect Real-Time PCR Detection System with a starter package (#1855200), containing SYBR Green Supermix, 96-well PCR plates and plate seals, which is a highly recommended option.