The generation of specialized cell types through in vitro stem cell differentiation has become valuable in areas such as development and regenerative medicine. Differentiation provides a source of viable, specialized cells for use in various research applications. A common example is the differentiation of mesenchymal stem cells (MSC) into new cell types like chondrocytes, adipocytes, and osteocytes. Depending on the type of stem cell culture, the differentiation process may involve components such as specific growth factors, extracellular matrix substrates, three dimensional environments, and certain inhibitors. For convenience, several commercial manufacturers have developed complete media reagents and kits for differentiation of specific cell types.