Anti-RAD53 antibodies are used in antigen-specific immunodetection in biological samples. The target RAD53 is a known alias name of the protein checkpoint kinase 2, encoded by the CHEK2 gene in humans. This 543-amino acid residue protein is reported to be a serine/threonine-protein kinase, which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. It is localized to the nucleus of the cell and features ubiquitinated and phosphorylated post-translational modifications. It is reported to be widely expressed in many tissue types. Other synonyms for RAD53 include CDS1, CHK2, and HuCds1. Over 60 citations in the literature describe the use of RAD53 antibodies in research. Western Blot is a widely used application for these antibodies. ELISA and Immunohistochemistry are also common applications.