Anti-CHGA antibodies are used in the immunodetection of the protein chromogranin A. In humans, the canonical protein has a reported length of 457 amino acid residues and a mass of 50.7 kDa. Its subcellular localization is in the cytoplasmic vesicles and is secreted. It is notably expressed in cerebrospinal fluid. A member of the Chromogranin/secretogranin protein family, CHGA is known to be involved with innate immune responses. Post-translational modifications have been described, including O-glycosylation, protein cleavage and sulfation.
The CHGA marker can be used to identify Neuroendocrine Cells, Merkel Cells, Epithelium Lobar Bronchus Neuroendocrine Cells, Lung Neuroendocrine Cells, and Pancreatic Ductal Cells.* Synonyms for this target antigen include PHE5, PHES, SP-I, betagranin (N-terminal fragment of chromogranin A), catestatin, and CGA. CHGA gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. Over 440 citations in the literature describe the use of CHGA antibodies in research. Immunohistochemistry is a widely used application for these antibodies. Western Blot and Immunofluorescence are also common applications.
*HuBMAP Human Reference Atlas v1.4