Anti-KNL1 antibodies are used in the immunodetection of the protein kinetochore scaffold 1. In humans, the canonical protein has a reported length of 2342 amino acid residues and a mass of 265.4 kDa. Its subcellular localization is in the nucleus. Up to 4 different isoforms have been reported for this protein. It is reported to be highly expressed in the testis, where it is localized in germ cells, in particular in spermatocytes and in the pre-acrosome of round spermatids. KNL1 is known to be involved with cell division. The KNL1 gene has been associated with the disease, Microcephaly. Synonyms for this target antigen include blinkin, bub-linking kinetochore protein, cancer susceptibility candidate 5, cancer susceptibility candidate gene 5 protein, cancer/testis antigen 29, and ALL1-fused gene from chromosome 15q14 protein. KNL1 gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species.