Anti-HCRT antibodies are used in the immunodetection of the protein hypocretin neuropeptide precursor. In humans, the canonical protein has a reported length of 131 amino acid residues and a mass of 13.4 kDa. Its subcellular localization is in the cytoplasmic vesicles and ER. It is notably expressed in the hypothalamus. A member of the Orexin protein family, HCRT is known to be involved with chemical synaptic transmission and DNA replication. Post-translational modifications have been described, including protein cleavage. The HCRT gene has been associated with the disease, Narcolepsy. Synonyms for this target antigen include OX, PPOX, hypocretin (orexin) neuropeptide, orexin, prepro-orexin, preprohypocretin, and NRCLP1. HCRT gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. A number of HCRT antibodies have been mentioned in research publications and have associated citations. Immunohistochemistry is a widely used application for these antibodies. ELISA and Western Blot are also common applications.