Anti-carbonic anhydrase 4 antibodies are used for the immunodetection of the protein encoded by the CA4 gene. In humans, the canonical protein has a reported length of 312 amino acid residues and a mass of 35 kDa. Its subcellular localization is in the cell membrane. Alternative splicing is reported to yield 2 different isoforms for this protein. It is reported to be expressed in the endothelium of the choriocapillaris in eyes. A member of the Alpha-carbonic anhydrase protein family, it is known to catalyze the reversible hydration of carbon dioxide into bicarbonate and protons and thus is essential to maintaining intracellular and extracellular pH.
The carbonic anhydrase 4 marker can be used to characterize Aerocyte Capillary (aCap) Cells.* Other names for this target antigen include Car4, RP17, CA-IV, carbonate dehydratase IV, carbonic anhydrase IV, carbonic dehydratase IV, and CAIV. Gene orthologs have been identified in the mouse, rat, bovine, frog, chimpanzee and chicken species. A number of carbonic anhydrase 4 antibodies have been mentioned in research publications and have associated citations. Western Blot is a widely used application for these antibodies. ELISA and Immunohistochemistry are also common applications.
*HuBMAP Human Reference Atlas v1.4