Anti-double homeobox 4 antibodies are used in the immunodetection of the protein encoded by the DUX4 gene. In humans, the canonical protein has a reported length of 424 amino acid residues and a mass of 44.9 kDa. Its subcellular localization is in the nucleus. Alternative splicing is reported to yield 2 different isoforms for this protein. A member of the Paired homeobox protein family, it is reported to be a transcription factor that is selectively and transiently expressed in cleavage-stage embryos. Other names for this target antigen include double homeobox protein 10, double homeobox protein 4/10, and double homeobox protein 4.